Chemical Composition andIn VitroEvaluation of Antioxidant Effect of Free Volatile Compounds FromSatureja montanaL.

Abstract

As a part of an investigation of natural antioxidants from Dalmatian aromatic plants, in this paper we report a study of the antioxidant activity related to the chemical composition of savory free volatile compounds. Twenty-one compounds were identified in the essential oil without fractionation, representing 97.4% of the total oil. The major compound was phenolic monoterpene thymol (45.2%). Other important compounds were monoterpenic hydrocarbons p-cymene (6.4%) a nd gamma-terpinene (5.9%)and oxygen-containing compounds carvacrol methyl ether (5.8%), thymol methyl ether (5.1%), carvacrol (5.3%), geraniol (5.0%) and borneol (3.9%). The evaluation of antioxidant power was performed in vitro by the beta-carotene bleaching and thiobarbituric acid (TBA) methods. As determined with both methods, the total savory essential oil as well as different fractions or pure constituents containing hydroxyl group exhibited relatively strong antioxidant effect. The hydrocarbons, when isolated as CH fraction, showed the poorest effectiveness in spite the fact that this fraction contained gamma-terpinene, alpha-terpinene, p-cymene and terpinolene which previously were identified as potential antioxidants.

A pharmacological and phytochemical overview onSatureja

“…Additionally, camphor was determined as the major compound of S. visianii Silic (Bezic et al, 2009;Dunkic & Bezic, 2008;Vidic et al, 2009). It is possible to increase the number of these kinds of examples as the following: isomenthone for S. boliviana (Dambolena et al, 2009;Hatami et al, 2011;Senatore et al, 1998), thymoquinone (Grosso et al, 2009b;Mastelic & Jerkovic, 2003;Palavra et al, 2011;Taborsky et al, 2012), geraniol (Cavar et al, 2008;Mastelic & Jerkovic, 2003;Radonic & Milos, 2003b), and carvacrol methyl ether (Serrano et al, 2011;Skocibusic & Bezic, 2004) for S. montana.…”

Chemical composition, antimicrobial, antioxidative and anticholinesterase activity of Satureja Montana L. ssp montana essential oil

“…The final density of bacterial and yeast's inoculum was 5 x 10 5 . Suspensions of the molds were made in sterile saline and their turbidity was confirmed by viable counting in a Thoma chamber.…”

“…Also, it is used as choleretic, stimulant, digestive, antiseptic for gastrointestinal tract [2] and for the treatment of premature ejaculation [3]. A number of investigations justified traditional utilization and have confirmed various biological effects of this plant species namely, antioxidant [4,5], antibacterial [4,[6][7][8][9][10] and antifungal [11]. Satureja montana essential oil showed antiproliferative activity on human erythroleukemic K562 cells [12].…”

Cytotoxic, anti-carcinogenic and antioxidant properties of the most frequent plant volatiles

“…carvacrol or thymol. Various techniques were used: 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging assay [63,69,70,71,72], β-carotene bleaching test [68,70,72], thiobarbituric acid reactive species (TBARS) assay [67,69,73], ferric ion reducing antioxidant power (FRAP) assay [72], oxygen radical absorbance capacity assay [74] and method measuring the formation of hydroperoxydienes from linoleic acid [67]. With help of electron spin resonance (ESR) spectroscopy Fujisawa et al [75] found that eugenol and eugenol-related synthetic compounds possessed radical scavenging activity comparable with the activity of conventional antioxidants.…”