Chemical composition and evaluation of possible alpha glucosidase inhibitory activity of eight Aloe species

Abstract

Many of the health benefits associated withPolysaccharides from the plants were isolated using hot extraction method and then hydrolyzed. The polysaccharide hydrolysates were identified using high performance liquid chromatographic technique. Maximum yield of total polysaccharides identified were obtained from A 7 (12.04%), A 1 (8.51%), A 8 (8.03%), A 2 (5.32%) and A 6 (2.18%) respectively. The isolated polysaccharides were tested for antihyperglycemic activity in alloxan-induced diabetic rats and alpha glucosidase inhibitory activity. Chromatographic investigation of the polysaccharides recorded the presence of 18 saccharides, glucuronic acid, stachyose, galacturonic acid, sucrose, glucose, xylose, galactose, rhamnose, mannose, arabinose, fructose polyol, mannitol and sorbitol in the eight Aloe species, but their quantitative composition differed among the species. Glucuronic acid, stachyose and galacturonic acid were the major detected saccharides. The results of the biological activities revealed significant antihyperglycemic activities with variable degrees. After four weeks of daily administration, polysaccharides isolated from A. vera (A 1 ) and A. arborescens (A 2 ) were the most active with 40 and 44% reduction in blood glucose level, respectively. All the tested polysaccharides showed significant alpha glucosidase inhibitory activity with IC50 (µg/ml) 11.70, 14.60 and 15.80 for A 7 , A 6 and A 1 respectively. In conclusion, the tested polysaccharides contribute to the antidiabetic action of these Aloe species.

Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew’s Mallow Stems as a Promising Anticancer Agent

“…The antioxidant activity achieved a substantial proportion of 67% due to the abundant presence of phenolic compounds and other dietary reducing agents. The findings of our study are consistent with those of Karimi et al [35], indicating a direct relationship between the number of phenolic compounds and their antioxidant capacity. For example, Lan [49] found that the level of chlorogenic acid in Flos Lonicerae extracts correlates with their antioxidant properties.…”

“…Sucrose and stachyose were the most abundant sugars (9.227 and 6.254 g 100 g − 1 of dry weight). Elsayed et al [ 35 ] reported that the types of sugars are analyzed through chromatography; the polysaccharides in the eight Aloe species contained 18 saccharides, glucuronic acid, stachyose, galacturonic acid, sucrose, glucose, xylose, galactose, rhamnose, mannose, arabinose, fructose, polyol, mannitol, and sorbitol. The quantitative distribution of these components varied across the different species.…”