Chemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)

Pereira, Kassia Martins Fernandes; Grecco, Simone dos Santos; Figueiredo, Carlos R.; Hosomi, Jorge Kioshi; Nakamura, Mari U.; Lago, João Henrique G.

doi:10.1177/1934578x1801300213

Cited by 8 publications

(13 citation statements)

References 28 publications

(39 reference statements)

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“…In this study, the behavioral effects of water-extracted LPB on ZF larvae were examined, whereas most of the previous in vitro and in vivo studies have examined the effects of BP on ethanol or methanol extracts in mice or rats [ 15 – 18 ]. The crude extract of LBP is mainly composed of cinnamic acids and flavonoids [ 4 , 5 ]; therefore, the characterization of the chemical profile of LBP is an important step in determining the quality of the extract. Here, the chemical composition of the LABP sample was verified by UPLC/DAD-ESI/HRMS/MS, and flavonoids and traces of bufadienolides were identified.…”

Section: Discussionmentioning

confidence: 99%

“…[ 4 ] identified the following LBP components through HPLC/MS/MS: cinnamic acids–caffeic and coumaroylglutaric acids; organic acids–malic, citric, and isoctric acids; and flavonoids–cyanidin-3-glucoside, dihydroquercetin, glucosyl arabinosyl rhamnosyl quercetin, isorhamnetin hexose pentose, miricetin-3-O-rhamnoside (miricitrin), quercetin 3- O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopiranoside, quercetin-3- O-α-L-rhamnopiranosíde (quercitrin), kaempferol 3-O-α-L-arabinopyranosyl -(1→2)-α-L-rhamnopyranoside (kapinnatoside), and isoscoparin-2”-O- arabinoside. In addition, LBP have demonstrated in vitro anticancer activity [ 4 ]. The leaves are used in infusions, juices, and pastes in traditional medicine to treat inflammations and infections [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

et al. 2022

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Bryophyllum pinnatum (Lam.) Oken (BP) is a plant that is used worldwide to treat inflammation, infections, anxiety, restlessness, and sleep disorders. While it is known that BP leaves are rich in flavonoids, the extent of the beneficial and toxic effects of its crude extracts remains unclear. Although some neurobehavioral studies using leaf extracts have been conducted, none has examined the effects of water-extracted leaf samples. The zebrafish is a powerful animal model used to gain insights into the efficacy and toxicity profiles of this plant due to its high fecundity, external development, and ease of performing behavioral assays. In this study, we performed behavioral testing after acute exposure to different concentrations of aqueous extract from leaves of B. pinnatum (LABP) on larval zebrafish, investigating light/dark preference, thigmotaxis, and locomotor activity parameters under both normal and stressed conditions. LABP demonstrated dose-and time-dependent biphasic effects on larval behavior. Acute exposure (25 min) to 500 mg/L LABP resulted in decreased locomotor activity. Exposure to 300 mg/L LABP during the sleep cycle decreased dark avoidance and thigmotaxis while increasing swimming velocity. After sleep deprivation, the group treated with 100 mg/L LABP showed decreased dark avoidance and increased velocity. After a heating stressor, the 30 mg/L and 300 mg/L LABP-treated groups showed decreased dark avoidance. These results suggest both anxiolytic and psychoactive effects of LABP in a dose-dependent manner in a larval zebrafish model. These findings provide a better understanding of the mechanisms underlying relevant behavioral effects, consequently supporting the safe and effective use of LABP for the treatment of mood disorders.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

et al. 2022

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“…Considering pregnancy as an experience of crisis, women are expected to show emotional instability. A multimodal approach could be of use in those cases and, in some cases, it is possible to use a medical prescription of Bryophyllum for anxiety and vitality restoration [18, 19]; psychotherapy (including families and partners, focusing on Antonovsky’s [20] sense of coherence; and reframing biographical facts for resilience improvement towards stress); relaxing procedures applied by a physiotherapist/nurse (massage, hot hydrotherapy without or with essential oil aromatherapy, breathing), and nutrition (eating style and habits orientation to promote better sleep).…”

Section: Discussionmentioning

confidence: 99%

Usual-Risk Pregnant Women’s Health-Related Quality of Life through Pregnancy and Puerperium with Anthroposophical Prenatal Care: A Retrospective Study

Götz¹,

Hosomi²,

Morais³

et al. 2021

Complement Med Res

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Introduction: Pregnancy is a physiological event that can lead to a decrease in quality of life. Symptoms within this period lack riskless treatment options. However, the anthroposophical integrative view points out the stimulation of vital energy recuperation as a possible inducer of changes in health perception for pregnant women. Objective: The aim was to observe the health-related quality of life variation in women during pregnancy and puerperium. The evaluation was based on the Medical Outcomes Study 36-Item Short-Form (SF-36). Method: This is a retrospective longitudinal study of SF-36 questionnaires administered at 3 different time points: at the first appointment, at an appointment between the 36th ± 1 week of pregnancy and at another appointment within the puerperium period. Results: Sixty-five patients completed the 3 questionnaires within the period of the study. The 8 domains of SF-36 and women’s behaviors throughout the prenatal and puerperal care period were analyzed. Quality of life decreased by the third trimester, with recuperation in the puerperium. Physical components were reduced significantly, while emotional and social components did not change significantly. Scores in mental health and vitality, as well as general health status, were higher in puerperium. Conclusion: Multiprofessional intervention broadened by the anthroposophical perspective fosters the health-related quality of life of women through gestation and puerperium.

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“…had 2.50 and 3.42% of total flavonoid. Details of B. pinnatum leaf compounds can be assessed in a research conducted by our research group [7].…”

Section: Methodsmentioning

confidence: 99%

“…Due to this widespread popular use of B. pinnatum for medicinal purposes, its chemical composition and pharmacological actions have been the subject of research. In short, the main chemical components identified in the leaves of B. pinnatum are steroids/bufadienolides, triterpenes, organic acids, hydrocarbons, and flavonoids [5][6][7]. In experimental in vitro and in vivo studies, B. pinnatum has been found to act on uterine contractility, exert sedative, muscle-relaxant, hepatoprotective, and immunomodulatory activities, and to produce analgesic, anti-inflammatory, and antidiabetic effects, among others [3,4,[8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Effects of Bryophyllum pinnatum Administration on Wistar Rat Pregnancy: Biochemical and Histological Aspects

et al. 2021

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Introduction: Bryophyllum pinnatum is widely used in folk medicine. It has neuropharmacological, anti-inflammatory, immunomodulatory, antidiabetic, hepatoprotective, and nephroprotective effects, among others. It also acts on uterine contractility. It is prescribed by practitioners of anthroposophic medicine for preterm labor, insomnia, and emotional disorders, and has other potential uses in obstetrics. As all drugs currently used in preterm labor have side effects, new tocolytic agents remain an area of active research. Objective: To evaluate the effect of B. pinnatum mother tincture (MT) on albino rats and their offspring throughout pregnancy from a biochemical and histological standpoint. Methods: Longitudinal, prospective, randomized controlled bioassay. This is the second stage of a trial that investigated 60 animals distributed across six equal groups: controls C1 and C2, which received 1 and 25 times the vehicle dose (30% ethanol), B1 and B2 (1- and 25-fold doses of B. pinnatum MT), and B3 and B4 (which received 50- and 100-fold doses of B. pinnatum concentrate). At this stage, blood chemistry parameters (glucose, alanine aminotransferase [ALT], aspartate aminotransferase [AST], creatinine, and blood urea nitrogen) were measured in dams, as well as histological aspects of dam liver, kidney, placenta, and uterine tissue and fetal liver, kidney, heart, and brain. Results: No differences were found between group B1 (therapeutic dose) and its control C1 in relation to glucose, AST, ALT, and creatinine. Group B2 exhibited lower glucose levels than groups C1, B3, and B4. There was no difference in AST across groups. Groups B3 and B4 exhibited higher ALT levels than groups C1 and B1. Groups B1–B4 exhibited higher urea nitrogen levels than group C1. Creatinine levels were higher in groups B2 and B3 than group C1. On morphological evaluation, fatty infiltration of the liver was observed in the alcoholic vehicle control groups (C1 and C2). Conclusions: Daily administration of B. pinnatum at therapeutic doses (group B1) to pregnant albino rats appears to be safe, with reduced glucose at dose B2, elevated ALT at doses B3 and B4, and increased urea at doses B1 to B4 and creatinine at B2 and B3, but never exceeding the normal reference range. It was not associated with histological changes in specimens of the maternal or fetal structures of interest.

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Chemical Composition and Cytotoxicity of Kalanchoe pinnata Leaves Extracts prepared using Accelerated System Extraction (ASE)

Cited by 8 publications

References 28 publications

The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

The psychoactive effects of Bryophyllum pinnatum (Lam.) Oken leaves in young zebrafish

Usual-Risk Pregnant Women’s Health-Related Quality of Life through Pregnancy and Puerperium with Anthroposophical Prenatal Care: A Retrospective Study

Effects of <b><i>Bryophyllum pinnatum</i></b> Administration on Wistar Rat Pregnancy: Biochemical and Histological Aspects

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