Chemical composition and cytotoxic activity of the polysaccharide fractions in Sarcodon imbricatus (Basidiomycota)

Sułkowska-Ziaja, Katarzyna; Muszyńska, Bożena; Ekiert, Halina

doi:10.5586/am.2012.006

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…Sarcodon imbricatus (SI), belonging to the family of Basidiomycotina and Aphyllophorales , is an edible and medicinal fungus, widely distributed in Central Europe and in North America [ 18 ] and also produced in Tibet, Gansu, Anhui, northwestern Yunnan, and western Sichuan of China [ 19 ]. Although SI is described to show various pharmacological activities including anti-inflammation and anticancer in the folk, previous studies mainly focused on its chemical component analysis and polysaccharide isolation [ 20 , 21 ]. Our group has already confirmed the immunomodulatory property of S. imbricatus water extracts in the cyclophosphamide- (CTX-) induced immunosupressive mouse model, which is related to its modulation on oxidative stress [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise‐Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2‐Mediated Oxidative Stress

Wang

Zhang

et al. 2018

Oxidative Medicine and Cellular Longevity

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Sarcodon imbricatus (SI), a precious edible fungus, contains 35.22% of total sugar, 18.33% of total protein, 24 types of fatty acid, 16 types of amino acid, and 8 types of minerals. Encouragingly, it is rich in potential antioxidants such as total polyphenols (0.41%), total sterols (3.16%), and vitamins (0.44%). In the present study, the antifatigue properties of SI and its potential mechanisms of action were explored by the experiments on acute excise-treated mice and chronic fatigue syndrome (CFS) mice. SI (0.25, 0.5, and 1 g/kg) significantly enhanced exercise tolerance in the weight-loaded forced swimming test (FST) and rota-rod test (RRT) and reduced the immobility in the tail suspension test on CFS mice. SI markedly increased the levels of glycogen in the liver and adenosine triphosphate (ATP) in the liver and muscle and decreased the lactic acid (LD) and blood urea nitrogen (BUN) content in both acute swimming-treated mice and CFS mice. SI improved the endogenous cellular antioxidant enzyme contents in the two mouse models by improving the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels in serum, liver, and muscle, respectively. In CFS mice, the enhanced expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2), SOD1, SOD2, heme oxygenase-1 (HO-1), and catalase (CAT) in the liver were observed after a 32-day SI administration. Our data indicated that SI possessed antifatigue activity, which may be related to its ability to normalize energy metabolism and Nrf2-mediated oxidative stress. Consequently, SI can be expected to serve as a novel natural antifatigue supplement in health foods.

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Section: Introductionmentioning

confidence: 99%

Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise‐Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2‐Mediated Oxidative Stress

Wang

Zhang

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Samples were then injected into the HPLC system, equipped with a refraction-index (RI) detector. LC-NH 2 column (Sigma) was used for the chromatographic separation of reducing sugars [80]. Glucose, galactose, fucose, mannose, xylose, and arabinose (Sigma) were used as standards.…”

Section: Monosaccharide Compositionmentioning

confidence: 99%

Fucoidan and Alginate from the Brown Algae Colpomenia sinuosa and Their Combination with Vitamin C Trigger Apoptosis in Colon Cancer

et al. 2022

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Brown seaweeds are producers of bioactive molecules which are known to inhibit oncogenic growth. Here, we investigated the antioxidant, cytotoxic, and apoptotic effects of two polysaccharides from the brown algae Colpomenia sinuosa, namely fucoidan and alginate, in a panel of cancer cell lines and evaluated their effects when combined with vitamin C. Fucoidan and alginate were isolated from brown algae and characterized by HPLC, FTIR, and NMR spectroscopy. The results indicated that highly sulfated fucoidans had higher antioxidant and cytotoxic effects than alginate. Human colon cancer cells were the most sensitive to the algal treatments, with fucoidan having an IC50 value (618.9 µg/mL−1) lower than that of alginate (690 µg/mL−1). The production of reactive oxygen species was increased upon treatment of HCT-116 cells with fucoidan and alginate, which suggest that these compounds may trigger cell death via oxidative damage. The combination of fucoidan with vitamin C showed enhanced effects compared to treatment with fucoidan alone, as evidenced by the significant inhibitory effects on HCT-116 colon cancer cell viability. The combination of the algal polysaccharides with vitamin C caused enhanced degeneration in the nuclei of cells, as evidenced by DAPI staining and increased the subG1 population, suggesting the induction of cell death. Together, these results suggest that fucoidan and alginate from the brown algae C. sinuosa are promising anticancer compounds, particularly when used in combination with vitamin C.

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“…Recent studies have demonstrated that Sarcodon imbricatus is also important as an agent that stimulates neuronal growth and promotes brain health [8]. Our previous studies of S. imbricatus indicated antimicrobial activity against five strains of Gram-positive and Gram-negative bacteria, antiviral activity against HPV-1 virus, and cytotoxic activity toward B16 murine melanoma tumor cell lines, sarcoma XC, and human breast cancer cell line in vitro of selected polysaccharide fractions isolated from mycelial cultures of this fungal species [9,10].…”

Section: Introductionmentioning

confidence: 99%

Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions

et al. 2016

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The effect of carbon and nitrogen sources and initial pH and temperature of the medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic liquid culture was investigated. The optimal composition of the medium was found to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extract, and 0.3% KH 2 PO 4 at pH = 6 and incubation temperature of 20°C. In this condition the maximum biomass growth was observed, yielding 10.2 g L −1 of dry weight after 3-week of growth. The medium regarded as optimal for growth of S. imbricatus mycelium was used for the production of the biomass and further chemical analysis. The quantitative and qualitative composition of phenolic acids, fatty acids, and sterols were determined using chromatographic methods. The total content of phenolic acids was 1.86 mg × 100 g −1 DW, with the largest amount of protocatechuic acid (1.27 mg × 100 g −1 DW). Nineteen fatty acids were estimated, including five unsaturated fatty acids, e.g., oleic and α-linolenic acid. The analysis of sterols composition revealed the presence of ergosterol and ergosterol peroxide (197.7 and 200.47 mg × 100 g −1 DW, respectively). These compounds were isolated and confirmed by 1 H-NMR. Presented study constitutes the first report on the accumulation of substances (phenolic acids, fatty acids, and sterols) with multidirectional biological activity in the mycelial axenic culture of Sarcodon imbricatus.

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Chemical composition and cytotoxic activity of the polysaccharide fractions in Sarcodon imbricatus (Basidiomycota)

Cited by 8 publications

References 18 publications

Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise‐Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2‐Mediated Oxidative Stress

Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise‐Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2‐Mediated Oxidative Stress

Fucoidan and Alginate from the Brown Algae Colpomenia sinuosa and Their Combination with Vitamin C Trigger Apoptosis in Colon Cancer

Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions

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