Chemical Composition and Bioactivity of Salvadora persica Extracts against Some Potato Bacterial Pathogens

EL-Hefny, Mervat; Ali, Hayssam M.; Ashmawy, Nader A.; Salem, Mohamed Z. M.

doi:10.15376/biores.12.1.1835-1849

Cited by 18 publications

(10 citation statements)

References 34 publications

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“…The pathological behavior of the isolated bacterial cultures, as well as their cultural, morphological, and physiological characters conform to those known for all soft rot bacteria. On the basis of the obtained data, we could identify these isolates as PCC1 and Ds1, in the same way as many other researchers have in previous works [4,11,12,40,[53][54][55].…”

Section: Discussionmentioning

confidence: 79%

Bioactivity of Selected Phenolic Acids and Hexane Extracts from Bougainvilla spectabilis and Citharexylum spinosum on the Growth of Pectobacterium carotovorum and Dickeya solani Bacteria: An Opportunity to Save the Environment

et al. 2020

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Phenolic acids and natural extracts, as ecofriendly environmental agents, can be used as bio bactericides against the growth of plant pathogenic bacteria. In this study, isolation trails from infected potato tubers and stems that showed soft rot symptoms in fields revealed two soft rot bacterial isolates and were initially identified through morphological, physiological, and pathogenicity tests. The molecular characterization of these isolates via PCR, based on the 16S rRNA region, was carried out by an analysis of the DNA sequence via BLAST and Genbank, and showed that the soft rot bacterial isolates belong to Pectobacterium carotovorum subsp. carotovorum (PCC1) and Dickeya solani (Ds1). The in vitro results of the tested phenolic acids against the cultured bacterial isolates proved that concentrations of 800, 1600, and 3200 μg/mL were the most effective. Ferulic acid was the potent suppressive phenolic acid tested against the Ds1 isolate, with an inhibition zone ranging from 6.00 to 25.75 mm at different concentrations (25–3200 μg/mL), but had no effect until reaching a concentration of 100 μg/mL in the PCC1 isolate, followed by tannic acid, which ranged from 7.00 to 25.50 mm. On the other hand, tannic acid resulted in a significant decrease in the growth rate of the PCC1 isolate with a mean of 9.11 mm. Chlorogenic acid was not as effective as the rest of the phenolic acids compared with the control. The n-hexane oily extract (HeOE) from Bougainvillea spectabilis bark showed the highest activity against PCC1 and Ds1, with inhibition zone values of 12 and 12.33 mm, respectively, at a concentration of 4000 μg/mL; while the HeOE from Citharexylum spinosum wood showed less activity. In the GC/MS analysis, nonanal, an oily liquid compound, was found ata percentage of 38.28%, followed by cis-2-nonenal (9.75%), which are the main compounds in B. spectabilis bark HeOE, and 2-undecenal (22.39%), trans-2-decenal (18.74%), and oleic acid (10.85%) were found, which are the main compounds in C. spinosum wood HeOE. In conclusion, the phenolic acids and plant HeOEs seem to raise the resistance of potato plants, improving their defense mechanisms against soft rot bacterial pathogens.

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Section: Discussionmentioning

confidence: 79%

Bioactivity of Selected Phenolic Acids and Hexane Extracts from Bougainvilla spectabilis and Citharexylum spinosum on the Growth of Pectobacterium carotovorum and Dickeya solani Bacteria: An Opportunity to Save the Environment

et al. 2020

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“…e previous phytochemical screening studies showed that the stem of S. persica yielded octacosanol, 1-triacantanol, β-sitosterol, 3-O-β-D-glucopyranoside salvadourea, m-anisic acid [22,23], pyrrolidine, pyrrole, and piperidine derivatives [24], salvadoside and salvadoraside glycosides [25], and flavonoids, which included quercetin, rutin, kaempferol, and quercetin glucoside [26], while the roots of S. persica yielded β-sitosteryl fatty esters and β-sitosteryl-3-vanilloyl-4′-fatty esters [27]. e roots, leaves, and stem contained ascorbic acid, butanediamide, salvadorine, salvadourea, lignans, fatty acids, trimethyl amine, cyanogenic glycosides, steroidal esters, tannins, aniline derivative, spiculesporic acid, methyl hexadecanoate, and homo-c-linolenic acid [28]. Additionally, the stem essential oil is reported to consist mainly of 1,8-cineole (eucalyptol) (46%), β-pinene (6.3%), and 9-epi-(E)-caryophyllene (13.4%) [29].…”

Section: Resultsmentioning

confidence: 99%

Phytochemical, Cytotoxic, and Antimicrobial Evaluation of the Fruits of Miswak Plant, Salvadora persica L.

Bratty

Makeen

Alhazmi

et al. 2020

Journal of Chemistry

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Salvadora persica L. (Salvadoraceae) is an evergreen shrub growing in the Middle East, Africa, and Southern Asia. It is traditionally known as “miswak” and used as toothbrushes and for the treatment of toothache, gum diseases, boils, chest infection, gonorrhea, headache, spleen troubles, stomachache, and ulcers. To the best of our knowledge, this is the first study aimed at conducting phytochemical, cytotoxic, and antimicrobial investigations of the fruits (berries) of S. persica collected from the Jazan region of Saudi Arabia. Analysis of the ethanol extract of S. persica fruits using GC-MS showed the presence of six esters (20.71%), seven alkanes (15.47%), tetracosamethyl-cyclododecasiloxane (9.91%), eicosamethyl-cyclodecasiloxane (7.27%), and 1-monolinoleoylglycerol (5.17%). The predominant constituents were acetyl dasycarpidan-1-methanol (10.47%), tetracosamethyl-cyclododecasiloxane (9.91%), eicosamethyl-cyclodecasiloxane (7.27%), and 1-monolinoleoylglycerol (5.17%). The petroleum ether extract of the fruits contained mainly eicosamethyl-cyclodecasiloxane (23.81%), 1-monolinoleoylglycerol (11.78%), (Z,Z,Z)-9,12,15-octadecatrienoic acid ethyl ester derivative (10.56%), and tetracosamethyl-cyclododecasiloxane (9.91%). The cytotoxic properties of the ethanol extract were investigated by MTT assay against the breast MCF7, ovary A2780, and colon HT29 cells. The fruit extract of S. persica was selective against the ovarian and colon cancer cells compared to normal fibroblast cells (MRC5) as it showed IC50 values 17.50, 8.35, and 5.12, against MCF7, A2780, and HT29 cells, respectively. Interestingly, the fruit extract was also found to possess selective antimicrobial activity for Streptococcus mutans isolates with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 3.12 and 6.25 mg/mL, respectively. Interestingly, it was found to be ineffective against other Gram-positive as well as Gram-negative microorganisms. This study provides insight into the bioactive components present in the fruits of the plant that can be utilized for its cytotoxic and antimicrobial properties.

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“…This inding was in accordance to the previous study done by Alamri et al (2018) where Miswak hexane extract exhibit potent antibacterial properties towards Gram-positive bacteria like S. aureus (Alamri et al, 2018). The extracts were reportedly contained, as primary parts, benzeneacetonitrile (71.47%), 4-aminocarbonyl-5-luoro-1-α-D-ribofuranosyl-imidazole (10.99%), and benzyl isothiocyanate (5.05%) (EL-Hefny et al, 2017).…”

Section: Antibacterial Activity Of Miswak Extractmentioning

confidence: 99%

Miswak starch-based gel as topical preparation for potential management of infective ulceration

Halib¹,

Hon²,

Hanafiah³

et al. 2021

ijrps

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Salvadora persica or Miswak is known for its antibacterial activities towards various bacteria species, including several oral pathogens. Although Miswak has many therapeutic potentials, the delivery medium is vital to ensure that the desired effects can be delivered to the target site. Ideally, starch is a biopolymer that exhibits muco-adhesive property, suitable as topical delivery material. Hence, this study was done to develop starch-based gel for Miswak antibacterial compound delivery system. In this study, Miswak hexane extract was incorporated into starch-based gels and stored at 4⁰C and room temperature. Miswak gel was tested for its antibacterial activity, and its stability was monitored by visual observation. As a result, the mean inhibitory zones of Miswak gels were found to be 22.67 ± 3.79 mm (4°C) and 20.67 ± 2.08 mm (room temperature) at day-1. The inhibitory zone decreased after 30 days with 13.00 ± 2.52 mm (4°C), and 12.00 ± 1.00 mm (room temperature). It was observed that the gels were stable where there were no colour changes, no phase separation and degradation, visualised up to day-60. In conclusion, the starch-based gel is a suitable delivery system for Miswak extract, thus as potential management for infective ulceration.

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Chemical Composition and Bioactivity of Salvadora persica Extracts against Some Potato Bacterial Pathogens

Cited by 18 publications

References 34 publications

Bioactivity of Selected Phenolic Acids and Hexane Extracts from Bougainvilla spectabilis and Citharexylum spinosum on the Growth of Pectobacterium carotovorum and Dickeya solani Bacteria: An Opportunity to Save the Environment

Bioactivity of Selected Phenolic Acids and Hexane Extracts from Bougainvilla spectabilis and Citharexylum spinosum on the Growth of Pectobacterium carotovorum and Dickeya solani Bacteria: An Opportunity to Save the Environment

Phytochemical, Cytotoxic, and Antimicrobial Evaluation of the Fruits of Miswak Plant, Salvadora persica L.

Miswak starch-based gel as topical preparation for potential management of infective ulceration

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