Ruku-ruku leaves are one of the natural resources in West Sumatra. However, the use of ruku-ruku leaves nowadays has only been used to enrich the taste of cuisine such as fish curry dishes. The distinctive odor of the ruku-ruku leaves can eliminate the fishy odor in the fish. This distinctive odor comes from secondary metabolite compounds on the leaves of the ruku-ruku. Based on previous research, the content of secondary metabolites can be differentiated from one region to another. In fact, to maximise its function, secondary metabolites are first identified and tested for biological activity. Therefore, this study identified secondary metabolites in the ruku-ruku leaves. The results showed that there were about 17 secondary metabolite compounds present in the ruku-ruku leaves. Limonnen, geranyl acetate, and citronellal are the three compounds that have the highest percentage in ruku-ruku leaves, respectively, 59.04%, 14.80%, and 7.81%. In addition, secondary metabolites present in the ruku-ruku leaves have biological activities such as antibacterial properties.