Chemical Composition and Antioxidant Effect of Mentha rotundifolia Extracts

Abstract

Aim: This report was aimed to investigate both the chemical composition and the antioxidant activity of two extracts from Mentha rotundifolia aerial parts. Methods: Aqueous and acetonic extracts were obtained by decoction and Soxhlet apparatus, respectively. Total polyphenols contents were determined using Folin-Ciocalteu reagent whereas flavonoids were evaluated by AlCl 3 method. LC-MS/MS method was carried out to reveal the phytochemical composition of extracts. Some tests were used to evaluate the in vitro antioxidant activity of extracts such as DPPH free radical scavenging assay, ABTS + radical cation decolorization assay, OH • radical scavenging assay, ferrous ion chelating, reducing power and β-caroten/linoleic acid bleaching assay. Results: The results showed that the aqueous extract was richer on polyphenols and flavonoids comparing to the acetonic extract. The analysis by LC-MS/MS allowed to identify many phenolic compounds in extracts, predominated by rosmarinic acid. In addition, the extracts were found to possess a significant antioxidant activity. Conclusion: Accordingly, it can be concluded that M. rotundifolia is quite rich in phenolic compounds and has a good antioxidant activity.

Recent advances in genus Mentha: Phytochemistry, antimicrobial effects, and food applications

“…EOs: β-caryophyllene (26.67%), germacrene D (12.31%), carveol (7.38%), pulegone (32.09%), piperitenone oxide (17.28%), and 5-acetyl thiazole (11.26%) (Riahia et al, 2013); 2,4(8),6-p-menthatrien-2,3-diol (14.5%) and germacrene D (12.4%) (Pino, Rosado, & Fuentes, 1999); piperitenone oxide (58.7%), piperitone oxide (15.9%), terpinen-4-ol (6.3%), and pulegone (3.9%) (Fatiha et al, 2016a); piperitenone oxide (75.6-68.7%), trans-caryophyllene (5.2%), and germacrene D (7.1%) (Retta et al, 2017); phenolic compounds and flavonoids: quercitrin, caftaric, gentisic, caffeic, p-coumaric, chlorogenic acids, rutin, isoquercitrin, luteolin, and apigenin (Moldovan et al, 2014); apigenin, isoquercetin, epicatechin, quercetin 3-O-galactoside, luteolin, chlorogenic acid, cyaninchlorid, myrtillin, quercetin, rutin, caffeic acid, ferulic acid, hydroxybenzoic acid, p-coumaric acid, rosmarinic acid, syringic acid, transcinamaldehyde acid, and vanilic acid (Siham, Rachid, & Al-Zoubi, 2019).…”

“…Antioxidant, antimicrobial, tonic, stimulative, stomachic, carminative, analgesic, choleritic, antispasmodic, antiemetic, anti-diarrhea, antihemorrhoidal, anti-inflammatory, sedative, hypotensive and insecticidal (Fatiha et al, 2016(Fatiha et al, , 2016aMoldovan et al, 2014;Pino et al, 1999;Riahia et al, 2013;Siham et al, 2019) ( while herbalist use whole plant as carminative (Chauhan et al, 2009). In Southeast Asian countries the plant is commonly used for culinary purposes and as a digestive.…”

Mentha rotundifolia (L.) Huds. aqueous extract attenuates H2O2 induced oxidative stress and neurotoxicity

“…Kaempferol and its derivatives are flavonols with promising bioactivities including anti-cancer, antiviral, antimicrobial, and neuroprotective properties (Bangar et al, 2022). Additionally, all these compounds have been shown to possess an important antioxidant activity (Sato et al, 2011;Uzun et al, 2017;Riahi et al, 2019), which may explain the strong antioxidant potential of M. rotundifolia aqueous extract in DPPH and ABTS assays, as it was previously confirmed (Fatiha et al, 2015;Siham et al, 2019).…”

“…In the current work, kaempferol glucuronide (accounted for 85%) dominated the extract along with other minor compounds, among them quinic acid, chlorogenic acid, caffeoyl shikimic acid, and kaempferol rutinoside. Noteworthy, rosmarinic acid was detected at the extract as a minor compound; however, it was among the major compounds from the Algerian and Tunisian species ( Fatiha et al, 2015 ; Siham et al, 2019 ). While eriocitrin was predominant in Saudi Arabian flora ( Alharbi et al, 2021 ).…”

“…Furthermore, it owns antioxidant, anti-tyrosinase, anti-acetylcholinesterase and insecticidal activities ( Fatiha et al, 2015 ; Ben Haj Yahia et al, 2019 ). These properties are mainly attributed to the presence of several phenolic compounds with rosmarinic acid as a dominant metabolite ( Siham et al, 2019 ).…”