Chemical composition and antioxidant activity of tronchuda cabbage internal leaves

Ferreres, Federico; Sousa, Carla; Vrchovská, Vendula; Valentão, Patrı́cia; Pereira, José Alberto; Seabra, Rosa M.; Andrade, Paula B.

doi:10.1007/s00217-005-0104-0

Cited by 95 publications

(112 citation statements)

References 44 publications

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“…Fragments are at m/z 265.0716 (loss of a part of hexose ring) and at m/z 223.0598 (sinapic acid). The MS fragmentation observed for these compounds was similar to that described in a previous work by Ferreres et al [24].…”

Section: Resultssupporting

confidence: 86%

Application of ultra performance liquid chromatography-photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS) method for the characterization of phenolic compounds of Lepidium sativum L. sprouts

Oszmiański

Kolniak‐Ostek

2013

Eur Food Res Technol

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Some of health-protecting phytochemicals can be found in the sprouts in a much higher concentration than in the developed plants. However, the polyphenol composition of Lepidium sativum L. sprouts is still unresolved, especially with regard to flavonoids and phenolic acids. To develop a rapid and reliable UPLC-PDA-Q/TOF-MS method for the identification and quantification of main flavonoids and phenolic acids from L. sativum sprouts, the samples of L. sativum sprouts were grown with and without the exposition to light. Ten phenolic compounds were identified on the basis of their mass spectra in MS mode, MS/MS spectra, PDA spectrum, and retention times as compared with available reference substances. The major compounds were found to be sinapic acid derivatives. Acylated flavonoids in L. sativum sprouts were identified as kaempferol and quercetin with the same glycosidic substitution pattern as the major compounds, and varying degrees of acylation with sinapic and ferulic acids. Total content of phenolics in both samples was similar, but the content of flavonoids was higher and sinapine was lower in the sample of sprouts germinated in light. The determination and the evaluation of L. sativum extracts is very effective while using the UPLC-PDA-Q/TOF-MS. Even though the substances only occur in low concentrations, the combination of the retention time and the accurate molecular mass allows for their fast qualification.

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Section: Resultssupporting

confidence: 86%

Application of ultra performance liquid chromatography-photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS) method for the characterization of phenolic compounds of Lepidium sativum L. sprouts

Oszmiański

Kolniak‐Ostek

2013

Eur Food Res Technol

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“…As observed with B. oleracea leaves and seeds [20,[30][31] shikimic (6) and fumaric (7) acids were present in small amounts, representing less than 0.5 and 2% of total acids, respectively ( Table 3). While fumaric acid showed behaviour similar to that exhibited by malic acid, shikimic acid displayed an opposite tendency, significantly decreasing until 6 days of germination.…”

supporting

confidence: 63%

“…While organic acids greatly increased, phenolic compounds had an opposite behaviour. Besides the depletion of the seeds phenolic compounds, the synthesis of the complex kaempferol derivatives, the predominant phenolic compounds in the leaves of mature plants [30,32], was not accomplished during this germination period. The lack of …”

Section: Discussionmentioning

confidence: 99%

“…The separation of the organic acids was carried out as previously reported [30], in a system consisted of an analytical HPLC unit (Gilson) with an ion exclusion column, Nucleogel Ion 300 OA (300 7.7 mm) (Macherey-Nagel, Düren, Germany) in conjunction with a column heating device set at 30°C. Briefly, elution was carried out isocratically at a solvent flow rate of 0.2 mL/min, with 0.01 M sulfuric acid.…”

Section: Hplc-uv Analysis Of Organic Acidsmentioning

confidence: 99%

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Screening of Antioxidant Compounds During Sprouting of Brassica oleracea L. var. costata DC

Sousa¹,

Lopes²,

Pereira³

et al. 2007

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Abstract:The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds, kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes in gentiobiose esters of sinapic acid were smaller. The total organic acids content increased rapidly during the first four days, with less significant variations thereafter. Malic acid, the major organic acid found in sprouts, greatly contributed to this result though oxalic, pyruvic, and fumaric acids also increased in the same manner. In contrast, aconitic, citric and shikimic acids showed decreases between days two and twelve of germination.

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“…Free radical scavenging is one of the known mechanisms by which antioxidants inhibit lipid oxidation. This test is a commonly employed assay in antioxidant studies of specific compounds or extracts over a short time scale (Ferreira et al, 2006;Ferreres et al, 2006). The RSA values of methanolic extracts from leaves sprayed with different copper compounds were examined (Fig.…”

Section: Radical-scavenging Activity (Rsa) Assaymentioning

confidence: 99%

Antioxidant activity and phenolic contents of Olea europaea L. leaves sprayed with different copper formulations

et al. 2007

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Olive trees (Olea europaea L. Cv. Cobrançosa) from the northeast of Portugal were sprayed with three different copper formulations [bordeaux mixture (copper sulphate + calcium hydroxide À 20% Cu), copper hydroxide (40% Cu) and copper oxychloride (50% Cu)] to control olive fungal diseases. The residues of copper in olive leaves, harvested at different times, were evaluated by atomic absorption spectrometry. At all the collection times, treated olive leaves had significantly higher copper contents, compared to the control. The different copper amounts in pesticide formulations lowered the leaves contents in total phenols and hence their antioxidant properties. Olive leaves sprayed with copper oxychloride possessed the highest copper levels and the lowest content in phenols, which influenced its antioxidant activity (higher EC 50 values for reducing power, scavenging effect on DPPH radicals and inhibition of erythrocyte hemolysis). Leaves without copper residues proved to be a good natural source of antioxidants, giving values comparable to the reference compounds.

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Chemical composition and antioxidant activity of tronchuda cabbage internal leaves

Cited by 95 publications

References 44 publications

Application of ultra performance liquid chromatography-photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS) method for the characterization of phenolic compounds of Lepidium sativum L. sprouts

Application of ultra performance liquid chromatography-photodiode detector-quadrupole/time of flight-mass spectrometry (UPLC-PDA-Q/TOF-MS) method for the characterization of phenolic compounds of Lepidium sativum L. sprouts

Screening of Antioxidant Compounds During Sprouting of Brassica oleracea L. var. costata DC

Antioxidant activity and phenolic contents of Olea europaea L. leaves sprayed with different copper formulations

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