Chemical Composition and Antioxidant Activity of Essential Oil Obtained from Bitter Orange Peel (Citrus aurantium L.) Using Two Methods

Radan, Mila; Parčina, Antonija; Burčul, Franko

doi:10.5562/cca3294

Cited by 17 publications

(16 citation statements)

References 14 publications

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“…The DPPH and ABTS methods are based on the stabilization of radicals, and the absence of activity is justified by the difficulty of the terpene compounds present in the essential oil to donate a hydrogen to neutralize the radical. In agreement with the data obtained in this work, Radan et al did not find antioxidant activity using these methods when they evaluated essential oils from peels from two varieties of bitter oranges ( Aurantii amari flavedo and Citrus aurantium L.). These oils also contained a predominance of limonene (91.1% and 51.3%, respectively).…”

Section: Resultssupporting

confidence: 89%

Biological activities of the essential oil from the Moro orange peel (Citrus sinensis (L.) Osbeck)

Magalhães

Ionta

Ferreira

et al. 2019

Flavour & Fragrance J

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Citrus essential oils have become the focus of several researches, because they have broad biological activity, due to their chemical composition. However, there are few studies covering Moro orange essential oil. Thus, the aim of this study was to evaluate the effects of fresh Moro orange peel essential oil on antifungal, antioxidant, cytotoxic, and genotoxic activities in normal and tumor cells. The essential oil was extracted by hydrodistillation and characterized by GC/MS and GC/FID. Cytotoxicity was evaluated by MTS assay in normal cells (CCD‐1059Sk) and tumor cells (CCD‐1059Sk), and the genotoxicity was determined in normal cells by the comet assay. Antifungal activity was evaluated by the disk diffusion test against Aspergillus carbonarius and Aspergillus flavus filamentous fungi. The antioxidant activity was determined in DPPH, ABTS, and β‐carotene assay. The essential oil was mainly composed of monoterpenes such as limonene (95.12%), α‐pinene (0.35%), sabinene (0.54%), and myrcene (1.07%). The essential oil did not cause cell death after 48 hours of treatment in normal cells, but the cell viability of tumor cells decreased by 50% in the presence of the essential oil at a concentration of 272.6 μg/mL. Although no antioxidant effect was observed, the growth of all evaluated fungi was inhibited with a minimum inhibitory concentration (MIC) of 125 μL/mL. The essential oil from the Moro orange is promising for cancer treatment, and it can be used as a natural preservative in food systems.

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Section: Resultssupporting

confidence: 89%

Biological activities of the essential oil from the Moro orange peel (Citrus sinensis (L.) Osbeck)

Magalhães

Ionta

Ferreira

et al. 2019

Flavour & Fragrance J

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“…Previous studies were focused on antimicrobial and health-promoting activities of different parts of C. aurantium , such as fruits and flowers, as well as essential oils obtained thereof [ 13 , 14 , 15 ]. Furthermore, substantial research has been focused on the chemical composition of essential oils prepared from various parts of Citrus aurantium growing in Pakistan [ 16 ], Brazil [ 17 , 18 ], Morocco [ 19 ], Iran [ 20 , 21 ], Croatia [ 22 ], northern Tunisia [ 23 ], India [ 24 ], and Algeria [ 25 , 26 ]. Essential oils of C. aurantium were reported to be a source of bioactive compounds with antimicrobial [ 19 , 21 , 23 , 27 ], antioxidant [ 19 , 22 , 23 ], anti-inflammatory [ 18 ], and anti-anxiety properties [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model

et al. 2020

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The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of Citrus aurantium essential oil (CAEO). The biofilm profile of Stenotrophonomonas maltophilia and Bacillus subtilis were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of Citrus aurantium (CAEO) was studied on wood and glass surfaces. A semi-quantitative composition using a modified version was applied for the CAEO characterization. The antioxidant activity of CAEO was determined using the DPPH method. The antimicrobial activity was analyzed by disc diffusion for two biofilm producing bacteria, while the vapor phase was used for three penicillia. The antibiofilm activity was observed with the agar microdilution method. The molecular differences of biofilm formation on different days were analyzed, and the genetic similarity was studied with dendrograms constructed from MSP spectra to illustrate the grouping profiles of S. maltophilia and B. subtilis. A differentiated branch was obtained for early growth variants of S. maltophilia for planktonic cells and all experimental groups. The time span can be reported for the grouping pattern of B. subtilis preferentially when comparing to the media matrix, but without clear differences among variants. Furthermore, the minimum inhibitory doses of the CAEO were investigated against microscopic fungi. The results showed that CAEO was most active against Penicillium crustosum, in the vapor phase, on bread and carrot in situ.

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“…In recent years, many studies have been focused to study chemical composition of the essential oils from various parts of Citrus aurantium growing in Pakistain (Kamal et al 2011), Brazil (Wolffendbuttel et al 2015Amorim et al 2016), Morocco (Ouedrhiria et al 2015), Iran (Khakpourk et al 2014;Azhdarzadeh et al 2016), Croatia (Radan et al 2018), northern Tunisia (Trabelsi et al 2014), India (Periyanayagam et al 2014) and Algeria (Abderrazak et al 2014;Djenane 2015). In addition, it has been reported that essential oils of C. aurantium were a source of bioactive compounds, they have been recognized as antimicrobial (Trabelsi et al 2014;Ouedrhiria et al 2015;Azhdarzadeh et al 2016), antioxidant (Trabelsi et al 2014;Ouedrhiria et al 2015;Radan et al 2018), antiinflammatory (Amorim et al 2016) and anti-anxiety agent (Khakpour et al 2014).…”

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confidence: 99%

Chemical composition, antimicrobial and insecticidal activities of the tunisian Citrus aurantium essential oils

Bnina¹,

Hajlaoui²,

Chaieb³

et al. 2019

Czech J. Food Sci.

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The chemical composition of volatile fractions from leaves, flowers and peels of Citrus aurantium growing in Tunisia obtained by hydrodistillation were analysed using GC and GC-MS. Furthermore, the isolated essential oils were evaluated for their in vitro antimicrobial activity against eight bacteria, eight phytopathogenic and nine human pathogenic fungi. The essential oils from peels and its main compound limonene have been found to possess strong contact toxicity against four storage-grain insects. The highest mortality rate was observed when the essential oil was applied against Cryptolestes ferrugineus, Liposcelis bostrychophila and Tribolium castaneum.

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Chemical Composition and Antioxidant Activity of Essential Oil Obtained from Bitter Orange Peel (Citrus aurantium L.) Using Two Methods

Cited by 17 publications

References 14 publications

Biological activities of the essential oil from the Moro orange peel (Citrus sinensis (L.) Osbeck)

Biological activities of the essential oil from the Moro orange peel (Citrus sinensis (L.) Osbeck)

Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model

Chemical composition, antimicrobial and insecticidal activities of the tunisian Citrus aurantium essential oils

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