Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf

Insuan, Wimonrut; Chahomchuen, Thippayarat

doi:10.4014/mbl.1912.12016

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…Although E citriodora essential oil has not been studied for its antiviral effect, previous studies have reported its antifungal and antimicrobial properties. 27,28 It was demonstrated in previous antiviral studies that citronellal may be the compound responsible for bioactivity. 29,30 The antimicrobial and antiviral effects of citronellal-rich essential oils were significant, especially against HSV-type viruses.…”

Section: Resultsmentioning

confidence: 99%

In vitro and in silico Evaluation of ACE2 and LOX Inhibitory Activity of Eucalyptus Essential Oils, 1,8-Cineole, and Citronellal

Sakallı

Teralı²,

Karadağ

et al. 2022

Natural Product Communications

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Eucalyptus essential oils are well-known and used especially in upper respiratory tract pathologies or diseases as herbal drug preparations. In the present study, the in vitro angiotensin-converting enzyme 2 (ACE2) and lipoxygenase (LOX) enzyme inhibitory potentials of commercial Eucalyptus globulus Labill. and Eucalyptus citriodora Hook. essential oils were evaluated for their potential anti-coronavirus disease 2019 (COVID-19), and anti-inflammatory effects. In addition, the major components, 1,8-cineole and citronellal, were evaluated for their ability to bind at the active site of either human ACE2 or human 5-LOX using an in silico setting. Before activity evaluation, Eucalyptus globulus and E citriodora essential oils were analysed by GC/FID and GC/MS, where 1,8-cineole (30%), and citronellal (80%) were identified as the major components, respectively. The in vitro ACE2 inhibition was calculated as 94.9% for E globulus, and that of E citriodora essential oil as 83.4%. In vitro LOX inhibition experiments for essential oils in the same order showed inhibitions of 71.3 and 91.4%, respectively, at 20 µg/mL test concentrations in microplate-based fluorometric assays. In addition, protein–ligand docking, and interaction profiling was used to gain structural and mechanistic insights into the in silico ACE2 and LOX inhibitory potentials of the major Eucalyptus essential oil constituents, 1,8-cineole as well as citronellal. The resulting data supported the in vitro findings; however, further in vivo studies are needed to confirm the activity.

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Section: Resultsmentioning

confidence: 99%

In vitro and in silico Evaluation of ACE2 and LOX Inhibitory Activity of Eucalyptus Essential Oils, 1,8-Cineole, and Citronellal

Sakallı

Teralı²,

Karadağ

et al. 2022

Natural Product Communications

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“…Likewise, C. citriodora is reported to have antimicrobial properties (ESTANISLAU; BARROS; PEÑA, 2001, MAHMOUD;EBRAHIM;ALY, 2004, MISSANJO;MKWEZALAMBA, 2016, MUHAMMED;DADA;ALO, 2018, MIGUEL et al, 2018CHAHOMCHUEN, 2020), insecticide (NEGRINI et al, 2018) and anthelmintic (ARAÚJO-FILHO et al, 2019). According to Mulyaningsih et al (2011), some active and bactericidal compounds present in lemonscented gum essential oil are aromadendreno, 1,8-cineol, citronellal and citronellol, which explains the potential for inhibiting Xanthomonas spp.…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobial principles (ESTANISLAU; BARROS; PEÑA, 2001, MAHMOUD; EBRAHIM; ALY, 2004, MISSANJO;MKWEZALAMBA, 2016, MUHAMMED;DADA;ALO, 2018, MIGUEL et al, 2018CHAHOMCHUEN, 2020. ), insecticides (NEGRINI et al, 2018) and anthelmintic (ARAÚJO-FILHO et al, 2019) have already been described in this species.…”

Section: ________________________________________________________________________________ Introductionmentioning

confidence: 99%

In vitro inhibition of Xanthomonas spp. by lemon-scented gum extracts

Mendes¹,

Marques²

2021

Rev Agr Acad

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The aim of this work was to evaluate lemon-scented gum (Corymbia citriodora) extracts in inhibiting the in vitro growth of Xanthomonas spp. The treatments consisted of aqueous, infusion, alcoholic and boiling extracts, in addition to the control with antibiotics. The methodology used to assess the antagonism was the double layer diffusion. The extracts were tested at 50, 35 and 15% concentration. None of the extracts inhibited the growth of X. citri pv. glycines, only the antibiotic bacitracin. As for X. vesicatoria, it was observed that only the alcoholic extract of C. citriodora leaves inhibited its growth, with emphasis on the 35% alcoholic extract, in addition to the antibiotics streptomycin and bacitracin. The growth of X. campestris was inhibited by alcoholic and aqueous extracts, highlighted on 50% and 35% alcoholic and 50% aqueous extracts, as well as by streptomycin and penicillin. The aqueous and alcoholic extracts of lemon-scented gum showed potential in the in vitro control of Xanthomonas and new studies will be carried out with new concentrations and in vivo control of diseases caused by these bacteria.

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“…Thus, our data suggest a possible relationship between essential oil chemical components and species. The chemical composition of EOs can vary depending on the species, leaf age, harvesting period, geographical location, and extraction procedures (Siramon et al, 2013;Sebei et al, 2015;Barbosa et al, 2016;Lingan, 2018;Salem et al, 2018;Almasa et al, 2019;Insuan and Chahomchuen, 2020). The difference in chemical constituents of EOs is related to a difference in biological activity.…”

Section: Discussionmentioning

confidence: 99%

“…The Eucalyptus leaf essential oils (EOs) were dried with anhydrous sodium sulphate and stored in glass vials at -20°C and protected from light until use. The identification of volatile constituents was performed using a GCMS-QP2010 apparatus (Shimadzu, Kyoto, Japan) and a ZB-WAX plus capillary column (30 m x 0.25 mm, 0.25 µm film thickness; Zebron, CA, USA) and a method was modified from Insuan and Chahomchuen (2020). GC-MS was carried out using split injection (split ratio 1:50) for a 0.2 µL oil sample, with the following temperature settings: injector set at 230 °C, column set at 60°C, heating ramp of 5°C/min, final temperature of 230°C, and detector set at 250°C.…”

Section: Extraction Of Essential Oils and Gas Chromatography-mass Spectrometry (Gc-ms) Analysismentioning

confidence: 99%

Antioxidant and anti-inflammatory properties of essential oils from three Eucalyptus species

Insuan¹,

Thongchuai²,

Chaiwongsa³

et al. 2021

CMUJNS

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Eucalyptus essential oils are used as traditional medicines in many countries. The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of leaf essential oils extracted from three different Eucalyptus species on HepG2 and RAW264.7 cells. Essential oils were distilled from fresh leaf samples, and the chemical constituents were analyzed using gas chromatography–mass spectrometry. The antioxidant activities of essential oils were determined using ABTS and hydroxyl radical scavenging assays, and hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells. Additionally, lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory properties. The results revealed that Eucalyptus citriodora and Eucalyptus urophylla leaf essential oils had a high content of oxygenated monoterpenes, whereas Eucalyptus deglupta contained a high amount of monoterpene hydrocarbons. Essential oils extracted from the three Eucalyptus species showed antioxidant and anti-inflammatory activities. E. citriodora and E. urophylla leaf essential oils had strong antioxidant activity against H2O2-induced oxidative stress in human HepG2 cells. Additionally, E. citriodora leaf essential oil, which contains a high amount of citronellal, exhibited the most potent anti-inflammatory activity in LPS-activated RAW264.7 macrophages. The antioxidant and anti-inflammatory effects of essential oils depended on their chemical composition. A principal component analysis explained 100% of the variance was performed to construct three groups based on the chemical components and antioxidant and anti-inflammatory activities. This study suggests that E. citriodora leaf essential oil, which represents a good source of oxygenated monoterpenes, could be considered a potential phytochemical agent for the prevention of oxidative stress and inflammation. Keywords: Antioxidant, Anti-inflammatory effect, Eucalyptus essential oils, Gas chromatography–mass spectrometry, Principal component analysis

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Chemical Composition and Antimicrobial Activity of Essential Oil Extracted from Eucalyptus citriodora Leaf

Cited by 10 publications

References 32 publications

In vitro and in silico Evaluation of ACE2 and LOX Inhibitory Activity of Eucalyptus Essential Oils, 1,8-Cineole, and Citronellal

In vitro and in silico Evaluation of ACE2 and LOX Inhibitory Activity of Eucalyptus Essential Oils, 1,8-Cineole, and Citronellal

In vitro inhibition of Xanthomonas spp. by lemon-scented gum extracts

Antioxidant and anti-inflammatory properties of essential oils from three Eucalyptus species

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