Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites

Lee, Seongwei; Musa, Najiah; Wee, Wendy; Musa, Nadirah

doi:10.1007/s11703-009-0052-8

Cited by 53 publications

(38 citation statements)

References 19 publications

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“…Previous reports indicated that clove oil contained eugenol ($75%), -caryophyllene (&5%), eugenyl acetate (&16%), and other components 51% (Chaieb et al, 2007); analyses of the aqueous extracts showed it contained 49% eugenol (Lee et al, 2009). The extracts in the current study were assessed using the same GC-MS methods as that of Lee et al (2009); the data showed eugenol made up 74% of material in the ethanolic extract and 43% in aqueous extract.…”

Section: Analyses Of Extract Contentssupporting

confidence: 50%

“…The extracts in the current study were assessed using the same GC-MS methods as that of Lee et al (2009); the data showed eugenol made up 74% of material in the ethanolic extract and 43% in aqueous extract. Although we did not assess directly the levels of -caryophyllene and eugenyl acetate, we assume these are in line with the values reported in both Chaieb et al (2007) and Lee et al (2009) and, thus, comprise the bulk of the remaining materials in each extract.…”

Section: Analyses Of Extract Contentsmentioning

confidence: 99%

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Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Dibazar

Fateh

Daneshmandi

2014

Journal of Immunotoxicology

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The present work sought to investigate potential suppressive effects on mouse macrophages by in vitro treatment with clove (Syzygium aromaticum) ethanolic extracted essential oil (containing eugenol) or its water-soluble extract. Using doses (ranging from 0.001-1000 mg/ml) of each material freshly prepared in the laboratory, cell survival and production of nitric oxide (NO), tumor necrosis factor (TNF)-, interleukin (IL)-6, and IL-12 by the treated cells (that in all cases also had received LPS stimulation) were measured. Results indicated that, except at doses !100 mg/ml, viability was unaffected in all groups. NO release by LPS-stimulated macrophages was generally significantly suppressed by either material; in contrast, low (i.e. 0.001-1 mg/ml) doses of either extract class appeared to enhance NO release by non-LPS (unstimulated)-treated macrophages. Among LPS-stimulated cells, TNF release was also significantly affected by each extract; the ethanolic extract was suppressive at all doses tested, while the aqueous material was so up to 1 mg/ml and then became stimulatory. In contrast, nearly every dose of either extract appeared to stimulate IL-6 release from the LPS-treated cells. Effects on IL-12 production were overall inconsistent; in general, the ethanolic extract tended to be stimulatory of production by the LPS-treated cells. The data for the aqueous material showed no discernable pattern of effect. The results suggest that clove extracts do not have a distinct cytotoxic activity, but do impart potential anti-and pro-oxidant effects in cells, depending on their concentrations and on the activation state of the macrophages themselves at the time of exposure to the extracts. The impact of the extracts on macrophage cytokine release also displays a pattern of dose-relatedness.

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Section: Analyses Of Extract Contentssupporting

confidence: 50%

Section: Analyses Of Extract Contentsmentioning

confidence: 99%

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Dibazar

Fateh

Daneshmandi

2014

Journal of Immunotoxicology

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“…The chemical composition of essential oils from several species of Syzygium species was previously described in the literature, especially those from S. aqueum, S. samarangense and two varieties of S. malaccense; S. aromaticum; S. guineense; S. caryophyllatum and Eugenia jambolana. [8][9][10][11][12][13][14][15] Sesquiterpenes (hydrocarbon and oxygenated derivatives) were found to be the main class of volatile constituents possessing antibacterial, antifungal, anti-inflammatory and cytotoxic activities 16 in addition, monoterpenes and phenylpropanoids were also described in these oils. 17 A research work has been carried out to test the antioxidant activities of Eucalyptus globulus (Myrtaceae) and the results showed that the plant is extremely beneficial.…”

Section: Introductionmentioning

confidence: 99%

Chemical profiling of leaf essential oil, Antioxidant potential and Antibacterial activity of Syzygium lanceolatum (Lam.) Wt. & Arn. (Myrtaceae)

Muthumperumal

Nadarajan

Das

et al. 2016

FRA

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Introduction: The present study was aimed to analyse the chemical composition, antioxidant potential and antibacterial activity in the leaf essential oil of Syzygium lanceolatum Methods: The essential oil was isolated by hydrodistillation method using a Clevenger type apparatus. The chemical composition of leaf essential oil was analysed by GC-MS. Antioxidant activity was determined by DPPH and ABTS radical scavenging assay. Antibacterial potential was tested against six bacterial strains. Results: GC-MS analysis revealed 2,8-Dimethyl-7-methylene-1,8-nonadien-3-yne (9.77%), Germacrene D (7.45%) as the major constituents and Linalool, β-Selinene (0.03%), Santalol (0.02%) were present in trace amounts. Maximum free-radical scavenging activity (69.97%) was observed at 500 ppm by the essential oil of S. lanceolatum, while the synthetic antioxidant Gallic acid showed 90.93% inhibition at the same concentration. The IC 50 value of leaf essential oil and standard gallic acid were 219.24 ± 5.82 ppm and 159.84 ± 2.7 ppm respectively. In ABTS analysis, the oil showed the percentage inhibition in concentration dependent manner. 500 ppm concentration of oil showed 73.01% free-radical inhibition while the gallic acid exhibited 90.4% inhibition. The IC 50 value of leaf essential oil and standard were 169.68 ± 3.09 ppm and 217.09 ± 0.70 ppm respectively. Antibacterial activity of leaf essential oil was tested against six bacterial strains and it was effective against all the strains. Conclusion: A total of 106 compounds were identified from the leaf essential oil and alkynes were the major class of compound, followed by the sesquiterpenes. The DPPH radical scavenging assay confirmed the antioxidant potential of the essential oil. Antibacterial activity tests proved that it is a potent growth inhibitor of pathogenic microbes. The present study throws light on the bioactive composition of S. lancoelatum essential oil and further studies will confirm the mode of action of these compounds.

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“…Moreover, fishes used to produce immune compromised antagonistic to particular adaptable pathogenic bacteria [7,8]. The plant-based extracts are being treated against numerous fish diseases to induce innate behavior and immunity and also for controlling the spread of infection [9][10][11][12][13][14]. The plant-based extracts have been tried to control fish diseases, as a replacement for commercial antibiotics which defends the particular pathogenic bacteria against resistance [15,16].…”

Section: Introductionmentioning

confidence: 99%

In vitro Antimicrobial Activity of Azadirachta indica (Leaves) against Fish Pathogenic Bacteria Isolated from Naturally Infected Dawkinsia filamentosa (Blackspot barb)

Kavitha¹,

Raja²,

Kamaraj³

et al. 2017

Med Aromat Plants

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To examine the antibacterial activity of ethyl acetate, ethanol and methanol extracts of Azadirachta indica (A. indica) on fish pathogens viz., Aeromonas veronii, Aeromonas hydrophila, Acinetobacter junii, Acinetobacter tandoii, Acinetobacter spp. and Pseudomonas stutzeri isolated from diseased blackspot barb, Dawkinsia filamentosa. The naturally infected fish, D. filamentosa were collected from Mettur Dam and the associated bacteria in the ulcerative lesions were isolated, sequenced the amplified product and tentatively identified through sequence similarity. The leaves powder was extracted successively with 250 ml of ethyl acetate, ethanol and methanol solvents by using Soxhlet extractor for 8 h at a temperature not exceeding the boiling point of the solvent. Ethyl acetate, ethanol and methanol extracts were subjected to phytochemical analysis as well as the antimicrobial assay using agar well diffusion method. Totally six fish bacterial pathogens were isolated from the ulcerative of lesions blackspot barb fish (D. filamentosa). All the gene sequences were deposited in the NCBI and received their accession numbers. The isolated strains were: A. veronii (KX688046), P. stutzeri (KX721473), A. hydrophila (KX756709), A. spp. (KX775221), A. junii (KX756708) and A. tandoii (KX775222). The different crude extracts of A. indica showed significant inhibitory effects on all the isolates. In which, the ethyl acetate extract showed maximum zone of inhibition on against P. stutzeri (23 mm) A. junii (22 mm), A. hydrophila (19 mm) and A. tandoii (18 mm). The ethanol extract showed moderate zone of inhibition on A. junii (15 mm) and A. veronii (14 mm) and also the methanol extract on A. junii (15 mm) and A. veronii (14 mm). The present study revealed that the crude extracts A. indica leaves possesses significant antibacterial activity on isolated fish pathogens. The susceptibility test was conducted on isolates using antibiotics, selected based on their importance to human medicine and on fish production. To conclude the present findings the ethyl acetate leaf extracts could be used as potential sources of antimicrobial agents.

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Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites

Cited by 53 publications

References 19 publications

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Chemical profiling of leaf essential oil, Antioxidant potential and Antibacterial activity of Syzygium lanceolatum (Lam.) Wt. & Arn. (Myrtaceae)

In vitro Antimicrobial Activity of Azadirachta indica (Leaves) against Fish Pathogenic Bacteria Isolated from Naturally Infected Dawkinsia filamentosa (Blackspot barb)

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