Chemical Composition and Antibacterial Activity of<i>Origanum majorana</i>L. Essential Oil from the Venezuelan Andes

Ramos, Sulymar; Rojas, Luis; Lucena, María Eugenia; Meccia, Gina; Usubillaga, Alfredo

doi:10.1080/10412905.2011.9700481

Cited by 41 publications

(32 citation statements)

References 16 publications

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“…Representative LC-MS chromatograms and the compounds detected are shown in Figure S2A,B, respectively. As expected, compounds such as Terpinen-4-ol, alpha-Terpinol, Alpha-Pinene, Camphene, p-Cymol, B-Caryophyllene, Bicyclogermacrene and Neophytadiene were present in OMEO, in agreement with previous reports [15,[26][27][28][29].…”

Section: Hplc-ms Identification Of Constituents In Origanum Majorana supporting

confidence: 92%

Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

et al. 2020

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Colorectal cancer (CRC) is the third most common type of cancer in terms of incidence and mortality worldwide. Here we have investigated the anti-colon cancer potential of Origanum majorana essential oil (OMEO) and its underlying mechanisms of action. We showed that OMEO significantly inhibited the cellular viability and colony growth of human HT-29 colorectal cancer cells. OMEO induced protective autophagy, associated with downregulation of the mTOR/p70S6K pathway, and activated caspase-8 and caspase-9-dependent apoptosis. Blockade of autophagy with 3-methyladenine (3-MA) and chloroquine (CQ), two autophagy inhibitors, potentiated the OMEO-induced apoptotic cell death. Inversely, inhibition of apoptosis with the pan-caspase inhibitor, Z-VAD-FMK, significantly reduced cell death, suggesting that apoptosis represents the main mechanism of OMEO-induced cell death. Mechanistically, we found that OMEO induces protective autophagy and apoptotic cells death via the activation of the p38 MAPK signaling pathway. Pharmacological inhibition of p38 MAPK by the p38 inhibitors SB 202190 and SB 203580 not only significantly decreased apoptotic cell death, but also reduced the autophagy level in OMEO treated HT-29 cells. Strikingly, we found that OMEO also induces p38 MAPK-mediated caspase-dependent cleavage of p70S6K, a protein reported to be overexpressed in colon cancer and associated with drug resistance. Our findings suggest that OMEO inhibits colon cancer through p38 MAPK-mediated protective autophagy and apoptosis associated with caspase-dependent cleavage of p70S6K. To the best of our knowledge, this study is the first to report on the implications of the p38 MAPK signaling pathway in targeting p70S6K to caspase cleavage.

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Section: Hplc-ms Identification Of Constituents In Origanum Majorana supporting

confidence: 92%

Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

et al. 2020

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“…The analysis of the chemical composition of samples obtained from different geographical locations indicates that the biological activity is directly related to the concentration of the essential oil components, which may vary according to the region [4, 7, 24, 25]. Moreover, season, climate, stage of plant development at harvest, and the technique of extraction of the product may influence the quantity of the plant compounds [24, 26].…”

Section: Resultsmentioning

confidence: 99%

“…Rodrigues et al [27] and Vági et al [13] also reported the presence of terpenes as the major components of the O. majorana essential oil. Usually, terpinen-4-ol and γ -terpinene are described as the most abundant compounds in O. majorana essential oil and sabinene and α -terpinene are also observed [4, 7, 13, 24, 25]. …”

Section: Resultsmentioning

confidence: 99%

Origanum majoranaEssential Oil Lacks Mutagenic Activity in theSalmonella/Microsome and Micronucleus Assays

Dantas

Klein‐Júnior

Machado

et al. 2016

The Scientific World Journal

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The present study aimed to investigate the in vitro mutagenic activity of Origanum majorana essential oil. The most abundant compounds identified by GC-MS were γ-terpinene (25.73%), α-terpinene (17.35%), terpinen-4-ol (17.24%), and sabinene (10.8%). Mutagenicity was evaluated by the Salmonella/microsome test using the preincubation procedure on TA98, TA97a, TA100, TA102, and TA1535 Salmonella typhimurium strains, in the absence or in the presence of metabolic activation. Cytotoxicity was detected at concentrations higher than 0.04 μL/plate in the absence of S9 mix and higher than 0.08 μL/plate in the presence of S9 mix and no gene mutation increase was observed. For the in vitro mammalian cell micronucleus test, V79 Chinese hamster lung fibroblasts were used. Cytotoxicity was only observed at concentrations higher than or equal to 0.05 μg/mL. Moreover, when tested in noncytotoxic concentrations, O. majorana essential oil was not able to induce chromosome mutation. The results from this study therefore suggest that O. majorana essential oil is not mutagenic at the concentrations tested in the Salmonella/microsome and micronucleus assays.

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“…The scarce information that is available for the role of sabinene hydrate in tree defense and fungal inhibition (Ramos et al 2011;Tomlin et al 2000) suggests that this compound might have moderate defensive and antifungal activity. The role of camphor (Kotan et al 2007;Marei et al 2012;Pragadheesh et al 2013;Ramsewak et al 2003) is ambiguous, being inhibitory for some fungi but not others, suggesting slight fungal toxicity.…”

Section: Discussionmentioning

confidence: 99%

Strong Induction of Minor Terpenes in Italian Cypress, Cupressus sempervirens, in Response to Infection by the Fungus Seiridium cardinale

Achotegui-Castells

Danti²,

Llusià

et al. 2015

J Chem Ecol

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Seiridium cardinale, the main fungal pathogen responsible for cypress bark canker, is the largest threat to cypresses worldwide. The terpene response of canker-resistant clones of Italian cypress, Cupressus sempervirens, to two differently aggressive isolates of S. cardinale was studied. Phloem terpene concentrations, foliar terpene concentrations, as well as foliar terpene emission rates were analyzed 1, 10, 30, and 90 days after artificial inoculation with fungal isolates. The phloem surrounding the inoculation point exhibited de novo production of four oxygenated monoterpenes and two unidentified terpenes. The concentrations of several constitutive mono-and diterpenes increased strongly (especially α-thujene, sabinene, terpinolene, terpinen-4-ol, oxygenated monoterpenes, manool, and two unidentified diterpenes) as the infection progressed. The proportion of minor terpenes in the infected cypresses increased markedly from the first day after inoculation (from 10 % in the control to 30-50 % in the infected treatments). Foliar concentrations showed no clear trend, but emission rates peaked at day 10 in infected trees, with higher δ-3-carene (15-fold) and total monoterpene (10-fold) emissions than the control. No substantial differences were found among cypresses infected by the two fungal isolates. These results suggest that cypresses activate several direct and indirect chemical defense mechanisms after infection by S. cardinale.

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Chemical Composition and Antibacterial Activity ofOriganum majoranaL. Essential Oil from the Venezuelan Andes

Cited by 41 publications

References 16 publications

Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

Origanum majorana Essential Oil Triggers p38 MAPK-Mediated Protective Autophagy, Apoptosis, and Caspase-Dependent Cleavage of P70S6K in Colorectal Cancer Cells

Origanum majoranaEssential Oil Lacks Mutagenic Activity in theSalmonella/Microsome and Micronucleus Assays

Strong Induction of Minor Terpenes in Italian Cypress, Cupressus sempervirens, in Response to Infection by the Fungus Seiridium cardinale

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