Chemical characterization of isocyanate-protein conjugates

Tse, Chris; Aj, Pesce

doi:10.1016/0041-008x(79)90006-1

Cited by 66 publications

(28 citation statements)

References 8 publications

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“…The investigated workers neither exhibited asthmatic symptoms nor the aberrant serotonin kinetic characteristics for endogenous asthma. Organic isocyanates react in vitro with free amino groups and other sites in proteins (6,28). Hexarnethylenediisocyanate was present in the hardeners used by our workers.…”

Section: Discussionmentioning

confidence: 94%

“…The isocyanates react in vitro with free amino groups and other sites on protein molecules. The reaction products may be sirnilar to derivatives formed in vivo which cause immunogenic and/or allergenic symptoms in exposed workers (28). With hydrolysis hexamethylene diisocyanate, which was present in low amounts in the hardeners used by the workers in the present study, yields hexarnethylenediamine, a toxic substance, the fumes of which have been shown to cause respiratory difficulties in exposed rats (6).…”

mentioning

confidence: 93%

“…The isocyanates are toxic to mucosa membranes, especially conjunctiva and the respiratory tract (3,22,28). Long-term exposure to low concentrations of organic isocyanates may cause asthma-like reactions (3,16,17).…”

mentioning

confidence: 99%

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Increased uptake of serotonin in platelets from car painters occupationally exposed to mixtures of solvents and organic isocyanates.

Beving¹,

Malmgren²,

Olsson³

et al. 1983

Scand J Work Environ Health

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BEVING H, MALMGREN R, OLSSON P, TORNLING G, UNGE G. Increased uptake of serotonin in platelets from car painters occupationally exposed to mixtures of solvents and organic isocyanates. Scand j work environ health 9 (1983) 253-258. Twelve car painters occupationally exposed to mixtures of solvents and organic isocyanates were investigated concerning serotonin uptake in platelets. The data from the exposed workers were compared to data from a reference group consisting of 50 nonexposed volunteers. The mean platelet count in whole blood of the exposed workers was markedly lower than the corresponding value of the reference group. Three workers had values below the lower limit of the 95 C/c tolerance interval of the referents. In both groups the serotonin transport strictly obeyed Michaelis-Menten's simple saturation kinetics. Nine of the twelve exposed workers had uptake rates which were significantly higher (p = 0.05) than those of the referents. The obtained data suggest that the present method may be used to reveal early organ damage, prior to the appearance of clinical symptoms, due to intermittent exposure to organic solvents and isocyanates.

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Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 93%

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Increased uptake of serotonin in platelets from car painters occupationally exposed to mixtures of solvents and organic isocyanates.

Beving¹,

Malmgren²,

Olsson³

et al. 1983

Scand J Work Environ Health

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“…10 Isocyanates react rapidly with proteins in vitro and in vivo, and isocyanate-protein conjugates are believed to represent the major immunogenic form of the chemical. 6,9,11,12 In exposed humans, albumin in the airway fluid is a major target for isocyanate and isocyanate-albumin conjugates are the only known "isocyanate antigen" specifically recognized by rearranged antigen receptors of the adaptive immune system (i.e. IgG).…”

Section: Introductionmentioning

confidence: 99%

Human innate immune responses to hexamethylene diisocyanate (HDI) and HDI–albumin conjugates

Wisnewski

Liu

et al. 2008

Clin Experimental Allergy

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“…Lysine was our first choice, as in vitro studies had shown that, when added to proteins, isocyanates and anhydrides react with this amino acid (52,53). Lysine has an epsilon amino group which can take part in the reaction and has also been shown to be the most likely residue for the covalent binding of chloramine-T to a protein, although tyrosine, methionine, cysteine, and histidine were also modified by the substance (54).…”

mentioning

confidence: 99%