Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials

Sulaiman, Ghassan M.; Sammarrae, Khulood W. Al; Ad’hiah, Ali H.; Zucchetti, Massimo; Frapolli, Roberta; Bello, Ezia; Erba, Eugenio; D’Incalci, Maurizio; Bagnati, Renzo

doi:10.1016/j.fct.2011.06.060

Cited by 78 publications

(50 citation statements)

References 25 publications

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“…PEESG showed a dose-dependent scavenging activity for both DPPH (Figure 2(a)) and ABTS radicals (Figure 2(b)), as well as remarkable reducing power (Figure 2(c)). DPPH • and ABTS • are stable free radicals, which are frequently used for evaluation of a radical scavenging activity of natural compounds [21]. In the present study, PEESG exhibited no different scavenging capability of DPPH from that of ascorbic acid ( P > 0.05) and a significantly higher scavenging capability of ABTS than that of ascorbic acid ( P < 0.01).…”

Section: Resultscontrasting

confidence: 39%

Antioxidant and Anti-Inflammatory Activities of Phenolic-Enriched Extracts ofSmilax glabra

Zhu

Wang

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Smilax glabra Roxb. has been used for a long time as both food and folk medicine. In the present study, phenolic-enriched extract of S. glabra (PEESG) was extracted with 70% ethanol and purified by HP-20 column chromatography. Its antioxidant and anti-inflammatory activities were evaluated by radical scavenging assay, reducing power determination, and lipopolysaccharide (LPS)-induced RAW264.7 cells assays, respectively. PEESG exhibited obviously scavenging capacity for DPPH and ABTS radicals, as well as significant reducing power for ferric ion. Particularly, PEESG (12.5–50 μg/mL) showed a significantly higher efficiency for scavenging ABTS than that of ascorbic acid and no significant difference with ascorbic acid for DPPH scavenging. PEESG also possessed a significant suppression effect on proinflammatory mediators production, such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), in LPS-induced RAW264.7 cells. In addition, the main ingredients of PEESG were identified using ultrahigh pressure liquid chromatography coupled to electrospray mass spectrometry (U-HPLC-ESI-MS). Seventeen components, including 5-O-caffeoylshikimic acid, neoastilbin, astilbin, neoisoastilbin, isoastilbin, engetin and isoengeletin were identified. These findings strongly suggest the potential of PEESG as a natural antioxidant and anti-inflammatory agent.

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Section: Resultscontrasting

confidence: 39%

Antioxidant and Anti-Inflammatory Activities of Phenolic-Enriched Extracts ofSmilax glabra

Zhu

Wang

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Recently there have been a lot of publications and reviews that deal with the methodology of studying the chemical composition of apicultural products such as honey and propolis (Gómez-Caravaca et al, 2006;Sulaiman et al, 2011;Da Silva Frozza et al, 2012;Markiewicz-Żukowska et al, 2012). But, the chemical composition and antioxidant profile of beebread samples has not been studied uniformly.…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

Markiewicz‐Żukowska¹,

Naliwajko²,

Bartosiuk³

et al. 2013

Journal of Apicultural Science

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A b s t r a c t Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 µg/mL) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 -66%.

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“…Propolis has been used in folk medicines and complementary therapies since 3,000 BC in Egypt and has become one of the most popular functional foods all around the world. Propolis show a broad spectrum of bioactivities, such as antioxidant [6], antimicrobial [7], antitumor [8], antiviral [9], anti-ulcer [10], immunomodulatory [11], cardioprotective [12], and anti-inflammatory effects [13]. …”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms Underlying theIn VitroAnti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type)

Wang

Ping

Huang

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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China produces the greatest amount of propolis but there is still lack of basic studies on its pharmacological mechanisms. Our previous study found that ethanol extract from Chinese propolis (EECP) exerted excellent anti-inflammatory effects in vivo but mechanisms of action were elusive. To further clarify the possible mechanisms underlying the anti-inflammatory effects of Chinese propolis (poplar type), we utilized EECP to analyze its chemical composition and evaluated its potential anti-inflammatory effects in vitro. High-performance liquid chromatography (HPLC) profile indicated that EECP contained abundant flavonoids, including rutin, myricetin, quercetin, kaempferol, apigenin, pinocembrin, chrysin, and galangin. Next we found that EECP could significantly inhibit the production of NO, IL-1β, and IL-6 in lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells and suppress mRNA expression of iNOS, IL-1β, and IL-6 in a time- and dose-dependent manner. Furthermore, we found that EECP could suppress the phosphorylation of IκBα and AP-1 but did not affect IκBα's degradation. In addition, using a reporter assay, we found that EECP could block the activation of NF-κB in TNF-α-stimulated HEK 293T cells. Our findings give new insights for understanding the mechanisms involved in the anti-inflammatory effects by Chinese propolis and provide additional references for using propolis in alternative and complementary therapies.

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Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials

Cited by 78 publications

References 25 publications

Antioxidant and Anti-Inflammatory Activities of Phenolic-Enriched Extracts ofSmilax glabra

Antioxidant and Anti-Inflammatory Activities of Phenolic-Enriched Extracts ofSmilax glabra

Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

Molecular Mechanisms Underlying theIn VitroAnti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type)

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