Chemical characterization of constituents from <i>Polygonatum cyrtonema</i> Hua and their cytotoxic and antioxidant evaluation

Zeng, Ting; Tang, Yan-Ran; Li, Bin; Tasneem, Shumaila; Yuan, Huiping; Jia, Yan-Zhe; Daniyal, Muhammad; Hussain, Nusrat; Wang, Wenmiao; Zuo, Da-Lei; Gong, Limin; Liu, Bin; Shi, Ji-Lian; Zhou, Zhun; Peng, Chao; He, Shan

doi:10.1080/14786419.2018.1543682

Cited by 16 publications

(3 citation statements)

References 17 publications

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“…Currently, many TCM preparations containing Da Huang, have been clinically used for treating liver diseases, inflammation and cancer (Li et al, 2007;Li et al, 2009). Moreover, a large number of studies in vitro and in vivo have reported that Da Huang, TCM preparations containing Da Huang, the Da Huang extracts and the major anthraquinone compounds derived from Da Huang (such as emodin, rhein, aloe-emodin) have therapeutic potential in the treatment of hepatocellular carcinoma (Kupchan and Karim, 1976;Ha et al, 2004;Cao et al, 2005;Cha et al, 2005;Kim et al, 2005;Huang et al, 2007;Lu et al, 2007;Shi et al, 2008;Lin et al, 2009;Tsai et al, 2013;Lin et al, 2016;Lee et al, 2017;Tan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

Han

et al. 2021

Front. Pharmacol.

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Sennoside A (SA) is a bioactive component of Rheum officinale Baill. with an activity of irritant laxative, which has been reported to possess therapeutic potential in various diseases or conditions including obesity, insulin resistance, liver steatosis, prostate cancer and pancreatic cancer progression. However, whether SA has therapeutic potential in hepatocellular carcinoma (HCC) treatment remains elusive. In this study, we treated two HCC cell lines, HepG2 and SMMC-7721 with SA and found that SA selectively inhibited the growth of HCC cells by proliferation assay. SA has a good inhibitory effect on proliferation of HepG2 cells in a concentration dependent manner, but there was no effect on SMMC-7721 cells. Then we conducted transwell assays and transcriptome analysis in HCC cells and examined the effects of SA on HCC in vivo. The results showed that SA significantly inhibited the migration and invasion of HCC. Comparison of RNA-seq transcriptome profiles from control groups and SA-treated groups identified 171 and 264 differentially expressed genes (DEGs) in HepG2 and SMMC-7721 cells respectively, in which includes 2 overlapping up-regulated DEGs and 12 overlapping down-regulated DEGs between HepG2 and SMMC-7721 cells. The qPCR were applied to investigate the transcriptional level of 9 overlapping down-regulated DEGs related to cancer metastasis, and the results were consistent with RNA-seq data. The dominate pathways including Wnt signaling pathway, TNF signaling pathway, VEGF signaling pathway, and NF-κB signaling pathway were strongly inhibited by SA, which are involved in regulating cancer metastasis. Finally, we confirmed that the downregulation of KRT7 and KRT81 could inhibit HCC metastasis. This study has provided new insight into the understanding of the inhibitory effects and potential targets of SA on the metastasis of HCC.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

Han

et al. 2021

Front. Pharmacol.

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“…In TCM, PS was first recorded by Mingyi Bielu , and was listed to the “Top Grade” herb in Shennong Bencao Jing ( Zhao et al, 2018 ; Han et al, 2020 ). As a “medicine food homology” plant, many bioactive ingredients have been confirmed in PS, including polysaccharides, steroidal saponins, amino acids, flavonoids, anthraquinones, and alkaloids ( Zeng et al, 2020 ). Modern pharmacological studies have demonstrated that PS decreases blood glucose ( Ma et al, 2022 ) and blood pressure ( Su et al, 2022 ), prevents atherosclerosis ( Zhu et al, 2015 ), enhances immunity ( Sun et al, 2022 ), and has anti-fatigue ( Shen et al, 2021 ), anti-tumor ( Li et al, 2022 ), and anti-aging effects ( Chen et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-aging mechanism and effect of treatment with raw and wine-steamed Polygonatum sibiricum on D-galactose-induced aging in mice by inhibiting oxidative stress and modulating gut microbiota

Zhong,

Shen,

Fan

et al. 2024

Front. Pharmacol.

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BackgroundPolygonatum sibiricum (PS) is a traditional Chinese medicine (TCM) first recorded in Mingyi Bielu. The book documents that PS can nourish five internal organs, be taken for a long time, relax the body and prolong lifespan. Presently, PS is widely used in TCM to prevent premature graying of hair. Based on TCM theory and clinical trials, the wine steaming processed product from PS provides a better effect. However, no published study has elucidated the anti-aging mechanism.PurposeThe study aim was to investigate the anti-aging mechanism of PS and its wine steaming processed product in mice, specifically focusing on the effect of D-galactose (D-gal) surrounding the intestinal flora and the Kelch-like ECH-associated protein 1-nuclear factor erythroid 2-related factor 2-antioxidant response elements (Keap1/Nrf2/ARE) pathway.MethodsThe chemical components in Raw PS (RPS) and Wine-steamed PS (WPS) were identified by ultra-performance liquid chromatography–hybrid quadrupole-Orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). An aging model using Kunming mice was established through intraperitoneally injected D-gal. Concentrations of RPS and WPS at 5, 10, or 15 g/kg/day levels were administered intragastrically, respectively. The body weight, liver and spleen indexes, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) activities in serum and brain tissue were recorded. Hematoxylin and eosin (HE) stained brain tissue was histopathologically examined. The expressions of Keap1, Nrf2 and heme oxygenase 1 (HO-1) in the brain tissue at the mRNA and protein levels were respectively detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot (WB). Moreover, an Illumina Hiseq platform was used for 16S ribosomal RNA (16S rRNA) high-throughput sequencing to evaluate the proportions of intestinal flora in aging mice.ResultsThe proportions of saccharides, flavonoids, and triterpene acids were different between RPS and WPS. In the aging model mice, WPS outperformed RPS in improving body weight and mental state by increasing the spleen index, SOD and GSH-PX activities, decreasing the liver index and MDA activities, and restoring the histopathological morphology in D-gal-induced aging mice. At the mRNA levels, RPS and WPS significantly reduced the expression of Keap1 and increased the expressions of Nrf2 and HO-1. The trend in protein expressions was similar to that of the mRNA results, and WPS had a stronger effect than RPS. Fecal microbiota analysis showed that RPS and WPS restored intestinal microbiota proportions to normal levels.ConclusionThe results demonstrated that PS and its WPS had a positive effect in relieving oxidative stress in aging mice. WPS outperformed RPS, which might be related to the activation of the Keap1/Nrf2/ARE pathway and regulation of intestinal flora.

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“…Steroidal saponins are characterized by a skeleton derived from oxidosqualene, consisting of a sugar moiety linked to a steroidal (27 carbon atoms) aglycone [6]. Steroidal saponins have attracted extensive attention owing to their excellent anti-bacterial, anti-inflammatory, and anti-oxidation effect and the potential for alleviating diabetes and other metabolic disorders [5,7,8]. Steroidal saponins have been extensively used in the food, medicine, fodder, and cosmetics industries [9].…”

Section: Introductionmentioning

confidence: 99%

Extraction and identification of steroidal saponins from Polygonatum cyrtonema Hua using natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction method

Liu

Feng

Sui

et al. 2023

J of Separation Science

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In this study, quartz sand with particularly sharp nanoscale edges acted like a nanoscale knife physically cut cells of Polygonatum cyrtonema Hua into nanosized particles and was synergized with natural deep eutectic solvent to extract steroidal saponins of Polygonatum cyrtonema Hua. The natural deep eutectic solvent (choline chloride‐lactic acid)‐synergistic quartz sand‐assisted extraction was optimized using response surface methodology. The steroidal saponins purified with AB‐8 macroporous resin were identified using ultra‐high‐performance liquid chromatography‐triple time of flight mass spectrometry. The results showed that the experimental total saponins content value (36.97 ± 0.12 mg dioscin equivalent/g dry weight) at optimal extraction conditions with a temperature of 68°C, a rotational speed of 20 400 rpm, shear time of 4.3 min, the liquid‐solid ratio of 38 ml/g, was close to the maximum possible theoretical value (36.64 mg dioscin equivalent/g dry weight). A total of 20 steroidal saponins were identified, among which the content of (25R)‐Kingianoside E was the highest (102.66 ± 3.47 mg/g). Furthermore, a new steroid saponin (3β,25S)‐26‐(β‐D‐glucopyranosyloxy)‐22‐hydroxyfurost‐5‐en‐3‐yl 4‐O‐β‐D‐glucopyranosyl‐β‐D‐galactopyranoside+Glc was found for the first time. These results revealed that natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction was an efficient and green method to extract a variety of steroidal saponins.

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Chemical characterization of constituents from Polygonatum cyrtonema Hua and their cytotoxic and antioxidant evaluation

Cited by 16 publications

References 17 publications

Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

Transcriptome Analysis of the Inhibitory Effect of Sennoside A on the Metastasis of Hepatocellular Carcinoma Cells

Anti-aging mechanism and effect of treatment with raw and wine-steamed Polygonatum sibiricum on D-galactose-induced aging in mice by inhibiting oxidative stress and modulating gut microbiota

Extraction and identification of steroidal saponins from Polygonatum cyrtonema Hua using natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction method

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