Chemical characterization of a PD-1/PD-L1 inhibitory activity fraction of the ethanol extract from<i>Gymnadenia conopsea</i>

Lin, Peng-Cheng; Wang, Xin; Zhong, Xiangjian; Zhou, Na; Wu, Lei; Li, Jinjie; Hu, Yangtao; Shang, Xiao-Ya

doi:10.1080/10286020.2020.1844190

Cited by 1 publication

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“…An HPLC-diode array detection-tandem mass spectrometry method (HPLC-DAD-MSn) was initially established for the analysis of chemical fingerprints of Gym rhizomes and the rapid identification of major compounds ( 10 ). Lin et al ( 11 ) developed a sensitive ultra-high performance liquid chromatography (UPLC)-HRMS/MS method for the rapid screening and identification of compositions in bioactive fractions. Forty-six compounds were identified from the ethanolic extract of Gym by extraction ion chromatography (EIC).…”

Section: Introductionmentioning

confidence: 99%

Optimization of the extraction process and metabonomics analysis of uric acid-reducing active substances from Gymnadenia R.Br. and its protective effect on hyperuricemia zebrafish

Chen

Pubu²,

Zhang³

et al. 2022

Front. Nutr.

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BackgroundAs Gymnadenia R.Br. (Gym) has an obvious uric acid-lowering effect, but its specific bioactive substances and mechanism are still unclear. The key metabolites and pathways used by Gym to reduce uric acid (UA) were identify.MethodsAn optimized extraction process for urate-lowering active substances from Gym was firstly been carried out based on the xanthine oxidase (XOD) inhibition model in vitro; then, the Ultra-high-performance liquid chromatography and Q-Exactive mass spectrometry (UHPLC-QE-MS) based on non-targeted metabolomics analysis of Traditional Chinese Medicine were performed for comparison of Gym with ethanol concentration of 95% (low extraction rate but high XOD inhibition rate) and 75% (high extraction rate but low XOD inhibition rate), respectively; finally, the protective effect of ethanolic extract of Gym on zebrafish with Hyperuricemia (referred to as HUA zebrafish) was explored.ResultsWe found that the inhibition rate of Gym extract with 95% ethanol concentration on XOD was 84.02%, and the extraction rate was 4.32%. Interestingly, when the other conditions were the same, the XOD inhibition rate of the Gym extract with 75% ethanol concentration was 76.84%, and the extraction rate was 14.68%. A total of 539 metabolites were identified, among them, 162 different metabolites were screened, of which 123 were up-regulated and 39 were down-regulated. Besides significantly reducing the contents of UA, BUN, CRE, ROS, MDA, and XOD activity in HUA zebrafish by Gym and acutely reduce the activity of SOD.ConclusionAlong with the flavonoids, polyphenols, alkaloids, terpenoids, and phenylpropanoids, the ethanolic extract of Gym may be related to reduce the UA level of Gym.

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Section: Introductionmentioning

confidence: 99%