Chemical characterization of a marine conditioning film

Garg, Anita; Jain, Anand; Bhosle, N.B.

doi:10.1016/j.ibiod.2008.05.004

Cited by 38 publications

(20 citation statements)

References 31 publications

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“…the formation of biological layers on immersed surfaces. The first step is the formation of a conditioning film (within minutes/hours), which is made up of glycoproteins, humic molecules, lipids, nucleic acids, and polysaccharides, [12]. Successively, a slime formed mainly of bacteria and diatoms (unicellular algae) develops within a few days [13].…”

Section: Introductionmentioning

confidence: 99%

Potentiodynamic study of Al–Mg alloy with superhydrophobic coating in photobiologically active/not active natural seawater

Benedetti

Cirisano

Delucchi

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Section: Introductionmentioning

confidence: 99%

Potentiodynamic study of Al–Mg alloy with superhydrophobic coating in photobiologically active/not active natural seawater

Benedetti

Cirisano

Delucchi

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…In their exposure to sea water, those materials undergo chem-ical and biological conditioning known as the fouling process. Chemical conditioning produces a molecular fi lm comprising compounds including glycoproteins, humic material, proteins, lipids, nucleic acids, polysaccharides, aromatic amino acids and/or unspecifi ed macromolecules (GARG et al 2009 and the references therein), whereas biological conditioning supersedes with the accumulation of prokaryotic and eukaryotic unicellular organisms (RAILKIN 2004) incorporated in the matrix of extracellular polymers (DONLAN 2002). A newly formed conditioning matrix, or biofi lm, is mostly composed of bacteria and diatoms.…”

Section: Introductionmentioning

confidence: 99%

Development of periphytic diatoms on different artificial substrates in the Eastern Adriatic Sea

Nenadović¹,

Šarčević²,

Čižmek³

et al. 2015

Acta Botanica Croatica

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-The settling of diatoms as fouling organisms on a certain substrate is greatly infl uenced by substrate characteristics and the preferences of a diatom community and diatom species. A distinction among substrates can be made by analysing the specifi c abundance and composition of diatoms on different substrates. In this study, 11 different artificial substrates were exposed to a marine environment for a period of 30 days. Abundance and taxonomic composition of periphytic diatoms was determined on each of the substrates and on shoots of the marine seagrass Posidonia oceanica. The aim was to compare diatom community structure on different newly colonized surfaces. On all surfaces examined, periphytic diatoms were the pioneering organisms with differences in quantitative and qualitative composition on the different substrates. Taxonomic analysis of diatom communities on the substrates examined revealed 41 diatom taxa, with the dominant genera Cylindrotheca, Amphora, Nitzschia, Cocconeis and Navicula. Given that all the examined artifi cial substrates were solid materials, differences in the abundance and species composition of diatoms found between the materials point to the substrates' physical and chemical characteristics as a major infl uence on the fi nal settling of diatoms. Knowledge from investigating the settlement of fouling organisms on anthropogenic substrates can have future use in management of waste materials that end up in the marine environment.

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“…The biofouling process occurs in the natural environment in sequential steps. Within minutes of immersion, a pristine surface becomes 'conditioned' through the adsorption of organic layers of macromolecules [5,9,19,27]. The conditioned surface is later colonized by microorganisms, including bacteria, algae (especially diatoms), fungi and protozoa.…”

Section: Introductionmentioning

confidence: 99%

Bacterial assay for the rapid assessment of antifouling and fouling release properties of coatings and materials

D’Souza¹,

Bruin²,

Biersteker³

et al. 2009

J Ind Microbiol Biotechnol

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An assay has been developed to accurately quantify the growth and release behaviour of bacterial biofilms on several test reference materials and coatings, using the marine bacterium Cobetia marina as a model organism. The assay can be used to investigate the inhibition of bacterial growth and release properties of many surfaces when compared to a reference. The method is based upon the staining of attached bacterial cells with the nucleic acid-binding, green fluorescent SYTO 13 stain. A strong linear correlation exists between the fluorescence of the bacterial suspension measured (RFU) using a plate reader and the total bacterial count measured with epifluorescence microscopy. This relationship allows the fluorescent technique to be used for the quantification of bacterial cells attached to surfaces. As the bacteria proliferate on the surface over a period of time, the relative fluorescence unit (RFU) measured using the plate reader also shows an increase with time. This was observed on all three test surfaces (glass, Epikote and Silastic T2) over a period of 4 h of bacterial growth, followed by a release assay, which was carried out by the application of hydrodynamic shear forces using a custom-made rotary device. Different fixed rotor speeds were tested, and based on the release analysis, 12 knots was used to provide standard shear force. The assay developed was then applied for assessing three different antifouling coatings of different surface roughness. The novel assay allows the rapid and sensitive enumeration of attached bacteria directly on the coated surface. This is the first plate reader assay technique that allows estimation of irreversibly attached bacterial cells directly on the coated surface without their removal from the surface or extraction of a stain into solution.

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Chemical characterization of a marine conditioning film

Cited by 38 publications

References 31 publications

Potentiodynamic study of Al–Mg alloy with superhydrophobic coating in photobiologically active/not active natural seawater

Potentiodynamic study of Al–Mg alloy with superhydrophobic coating in photobiologically active/not active natural seawater

Development of periphytic diatoms on different artificial substrates in the Eastern Adriatic Sea

Bacterial assay for the rapid assessment of antifouling and fouling release properties of coatings and materials

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