Chemical Characterization of a Collagen-Derived Protein Hydrolysate and Biostimulant Activity Assessment of Its Peptidic Components

Abstract

Protein hydrolysates (PHs) are plant biostimulants consisting of oligopeptides and free amino acids exploited in agriculture to increase crop productivity. This work aimed to fractionate a commercial collagen-derived protein hydrolysate (CDPH) according to the molecular mass of the peptides and evaluate the bioactivity of different components. First, the CDPH was dialyzed and/or filtrated and analyzed on maize, showing that smaller compounds were particularly active in stimulating lateral root growth. The CDPH was then fractionated through fast protein liquid chromatography and tested on in vitro grown tomatoes proving that all the fractions were bioactive. Furthermore, these fractions were characterized by liquid chromatography–electrospray ionization–tandem mass spectrometry revealing a consensus sequence shared among the identified peptides. Based on this sequence, a synthetic peptide was produced. We assessed its structural similarity with the CDPH, the collagen, and polyproline type II helix by comparing the respective circular dichroism spectra and for the first time, we proved that a signature peptide was as bioactive as the whole CDPH.

Hidroxiapatita de cálcio na harmonização orofacial: uma revisão narrativa da literatura

“…Nesse contexto, os bioestimuladores de colágeno surgem como uma excelente opção para tratar os sinais do envelhecimento, promovendo uma resposta inflamatória que estimula a produção de colágeno perdido. Esses bioestimuladores atuam nas camadas mais profundas do tecido, visando obter uma pele mais jovem e revitalizada (Ambrosini et al, 2022).…”

Protein Hydrolysates from Crambe abyssinica Seed Cake as Potential Biostimulants for Root Development

“…Using high-throughput phenotyping and metabolomics approaches, they showed the growth promotor and stress alleviator effects of those hydrolysates through the coordinated action of plant growth regulators and antioxidant compounds, such as phenolics and carotenoids. Ambrosini et al showed that the removal of free amino acids and short peptides through filtration and dialyses of a commercial collagen-derived protein hydrolysate lowered its biostimulating effect on the roots of maize plants, grown in hydroponic solution, showing that smaller compounds were particularly active in lateral root development [75].…”

Proteomic Identification and Characterization of Collagen from Bactrian Camel (Camelus bactrianus) Hoof

“…Figure 4a shows that the emulsification stability of CHC continues to decrease with increasing concentration. This indicates that collagen surface tension increased due to macro-molecular aggregation and decreased hydrophobicity [33].…”

“…The sample also has a peaked shape in the 1340 cm −1 band, representing the CHC showing the characteristics of proline side-chain rocking vibration [2]. In addition, a C-N stretching vibration at 1409 cm −1 suggests an interaction between the amide group and the molecule of the triple helix structure [33]. No absorption band corresponding to the carboxyl functional group was found in CHC (1740-1720 cm −1 ), consistent with the lyophilized pure collagen results previously reported in the literature [19].…”