Chemical Characterization and Cytotoxic Potential of a Chloroform Fraction Obtained from Marine Plant Thalassia testudinum

Riera‐Romo, Mario; D, Marrero Delange; Hernandez-Balmaseda, I; González, Kethia; Pérez-Martínez, Dayana; Manso-Vargas, A; Labrada, Mayrel; Guerra, Rodeiro; W, Vanden Berghe

doi:10.4172/2157-7064.1000405

Cited by 4 publications

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“…Additionally, this work suggested that the cytotoxic effect of the extract is associated with OS, nuclear damage, and sustained hypercalcaemia in HepG2 cells (Rodeiro et al, 2018). Along the same line, a chloroform fraction from T. testudinum was found to elicit cytotoxicity and antiproliferative effects on A549 and EA.hy926 immortalized cells as well (Riera et al, 2018). Altogether, these results demonstrate promising antitumor properties of the polyphenolic fraction of T. testudinum (PF) and TB, which could be confirmed in a xenograft mice model, a relevant animal model of human cancer research.…”

Section: Introductionmentioning

confidence: 64%

“…By GC-MS methods, approximately 69 compounds of the non-polar fraction were identified, where fatty acids, mainly palmitic acid, were the major components. The cytotoxic potential of this chloroform fraction was observed in A549 and EA.hy926 (Riera et al, 2018). Many reports have demonstrated the cytotoxic activity of fatty acids from vegetal sources in tumoral cell lines, including colorectal cancer cells (Mileo and Miccadei, 2016;Yang et al, 2017;Pacheco et al, 2018;Devanadera et al, 2019).…”

Section: Discussionmentioning

confidence: 85%

“…Thalassia testudinum seagrass (T. testudinum) has potential therapeutic use (Garateix et al, 2011;Regalado et al, 2012;García et al, 2017;Riera et al, 2018;Rodeiro et al, 2018), which in a near future can be approached as a nutraceutical product. The phytochemical characterization of an aqueous-ethanolic extract as well as the polyphenolic fraction from T. testudinum grown in the Cuban coasts showed a complex mixture containing phenols, flavonoids, proanthocyanidins, among others structures with cytotoxic effects (Table 1) (Regalado et al, 2012;Rodeiro et al, 2018;Riera et al, 2018). The glycosylated sulphated flavone thalassiolin B (chrysoeriol 7-β-D-glucopyranosyl-2"sulphate) (TB) has been identified as the main component found in T. testudinum hydroethanolic extract (5.8% w/w) ( Figure 1) (Regalado et al, 2009;Garateix et al, 2011;Regalado et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model

Delgado-Roche¹,

González²,

Mesta

et al. 2020

Front. Pharmacol.

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Marine plants are important sources of pharmacologically active metabolites. The aim of the present work was to evaluate the cytotoxic and antitumor activity of a polyphenolic fraction obtained from Thalassia testudinum marine plant and thalassiolin B in human colorectal cancer cells. Human cancer cell lines, including HCT15, HCT116, SW260, and HT29 were treated with tested products for cytotoxicity evaluation by crystal violet assay. The potential proapoptotic effect of these natural products was assessed by flow cytometry in HCT15 cells at 48 h using Annexin V-FITC/propidium iodide. In addition, reactive oxygen species (ROS) generation was measured by fluorescence using DCFH-DA staining, and sulfhydryl concentration by spectrophotometry. The in vivo antitumor activity of the polyphenolic fraction (25 mg/kg) was evaluated in a xenograft model in nu/nu mice. In vivo proapoptotic effect was also evaluated by immunohistochemistry using anti-caspase 3 and anti-Bcl-2 antibodies. The results showed that tested products exert colorectal cancer cell cytotoxicity. Besides, the tested products induced a significant increase (p < 0.05) of intracellular ROS generation, and a depletion of sulfhydryl concentration in HCT15 cells. The polyphenolic fraction arrested tumor growth and induced apoptosis in the xenograft mice model. These results demonstrate the cytotoxic activity of T. testudinum metabolites associated, at least, with ROS overproduction and pro-apoptotic effects. Here we demonstrated for the first time the antitumor activity of a T. testudinum polar extract in a xenograft mice model. These results suggest the potential use of T. testudinum marine plant metabolites as adjuvant treatment in cancer therapy.

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Section: Introductionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model

Delgado-Roche¹,

González²,

Mesta

et al. 2020

Front. Pharmacol.

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“…The result showed that the chloroform fraction of the hydroethanolic extract from seagrass, T. testudinum, exhibited concentration-dependent cytotoxicity, and it was most toxic to the A549 cell line, with an IC50 of 20.4 µg/mL, followed by the EA. hy926 cell line, which had an IC50 of 248.4 µg/mL [14] .…”

Section: Therapeutic Applications Anticancer Activitymentioning

confidence: 99%

A mini review on biological activities of genus Thalassia: A marine seagrass

Patil,

Mallya

2023

J Pharmacogn Phytochem

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Marine organisms produce a wide range of secondary metabolites that show the potential for the development of new moieties for the treatment of various diseases. Recently, several studies on secondary metabolites obtained from marine seagrasses resulted in the identification of pharmacologically active constituents. Thalassia, a marine seagrass genus belonging to the Hydrocharitaceae family, consists of two therapeutically useful species, namely, Thalassia testudinum and Thalassia hemprichii. The phytoconstituents present in the Thalassia species showed a variety of chemical compounds with proven therapeutic applications, such as alkaloids (antioxidant, larvicidal activity), glycosides (anticancer, antioxidant, larvicidal activities), fatty acids (anticancer activity), flavone glycosides (Anticancer activity), tannins, phenols (antioxidant, anticancer activities), ketones (antiviral activity), sterols (antioxidant, antiviral activities), flavonoids (anticancer activity), sulphates (antibacterial activity). This review aims to assemble the pharmacological activities of T. testudinum and T. hemprichii of the Thalassia genus, their phytoconstituents, methods for extraction, pharmacological actions, and their mechanisms and IC50 values. Hence, the results highlight the potency of these two species as natural therapeutic molecules for the treatment of diseases.

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Chemical constituents of the Vietnamese plants Dalbergia tonkinensis Prain and Cratoxylum formosum (Jack) Dyer in Hook and their DPPH radical scavenging activities

et al. 2019

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Chemical Characterization and Cytotoxic Potential of a Chloroform Fraction Obtained from Marine Plant Thalassia testudinum

Cited by 4 publications

References 0 publications

Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model

Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model

A mini review on biological activities of genus Thalassia: A marine seagrass

Chemical constituents of the Vietnamese plants Dalbergia tonkinensis Prain and Cratoxylum formosum (Jack) Dyer in Hook and their DPPH radical scavenging activities

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