Chemical characterization and cytotoxic activity evaluation of Lebanese propolis

Noureddine, Hiba; Hage‐Sleiman, Rouba; Wehbi, Batoul; Fayyad‐Kazan, Hussein; Hayar, Salem; Traboulssi, Mohamad; Alyamani, Osama A; Faour, Wissam H.; El-Makhour, Yolla

doi:10.1016/j.biopha.2017.08.067

Cited by 39 publications

(34 citation statements)

References 61 publications

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“…It was reported that flavonoids were more abundant than phenolic acid including rutin (48), quercetin (26), genistein (70), kaempferol (25), chrysin (2), pinocembrin (3) and galangin (9). [110] Northern African Coast Egyptian propolis analyzed by GC/MS was a typical poplar propolis type as reported by [111] but with few differences such as the presence of di-and triterpenes but at very low concentrations. Samples from Upper and East Nile Delta in Egypt were mainly characterized by the presence aliphatic acids (lactic acid, succinic acid, stearic acid, oleic acid, palmitic acid), phenolic acids [benzoic (28), ferulic (7), caffeic (5), 3,4-dime-thoxycinnamic (29), cinnamic (21) and p-coumaric acids (6)] ( Figure 2), flavonoids [pinocembrin chalcone (71), pinocembrin (3) pinobankasin-3-acetate (4)] (Figure 1) and di-and triterpene, in lower amounts, were also detected (e. g., cycloartenol (72), α-amyrin (73), triterpenes of β-amyrin type (74)] (Figure 4).…”

Section: Levantine Coastsupporting

confidence: 56%

“…Regarding the Levantine coast there is scarce investigations regarding the propolis application, which can be due to the few countries number representing this area. In Lebanon, Noureddine et al [110] studied the antiproliferative and proapoptotic activity of Lebanese propolis and have found similar activity to those aforementioned regarding its cytotoxicity and antiproliferative effect.…”

Section: Application In Medicine Pharmacy and Bio-allied Sciencementioning

confidence: 66%

“…[17,61] The presence, with high concentration, of isocupressic acid (32), isopimaric acid (42), imbricatoloic acid (35), agathadiol (44), totarol (36), 13-epi-torulosal (37), abietic acid (54), dehydroabietic acid (53) ( Figure 4) were also mentioned. [17,61] Noureddine et al [110] explored propolis from South of Lebanon and have concluded that bioactive compounds present in the tested extracts showed a similarity to those of southern European coast, concerning the presence of flavonoids and phenolic acids. It was reported that flavonoids were more abundant than phenolic acid including rutin (48), quercetin (26), genistein (70), kaempferol (25), chrysin (2), pinocembrin (3) and galangin (9).…”

Section: Levantine Coastmentioning

confidence: 99%

See 2 more Smart Citations

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

El-Guendouz

Lyoussi

Miguel

2019

Chemistry & Biodiversity

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This review updates the information upon the chemical composition of propolis from all Mediterranean countries as well as their biological properties and applications. The non‐volatile fraction of propolis was characterized by the presence of phenolic acids and their esters and flavonoids. Nevertheless, in some countries, diterpenes were also present: Sicily (Italy), Croatia, Malta, Creta (Greece), Turkey, Cyprus, Egypt, Libya, Algeria and Morocco. The volatile fraction of propolis was characterized by the presence of benzoic acid and its esters, mono‐ and sesquiterpenes, being the oxygenated sesquiterpene β‐eudesmol characteristic of poplar propolis, whereas the hydrocarbon monoterpene α‐pinene has been related with the presence of conifers. Regardless the chemical composition, there are common biological properties attributed to propolis. Owing to these attributes, propolis has been target of study for applications in diverse areas, such as food, medicine and livestock.

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Section: Levantine Coastsupporting

confidence: 56%

Section: Application In Medicine Pharmacy and Bio-allied Sciencementioning

confidence: 66%

Section: Levantine Coastmentioning

confidence: 99%

See 1 more Smart Citation

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

El-Guendouz

Lyoussi

Miguel

2019

Chemistry & Biodiversity

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“…Ambi et al () associated the presence of flavonoids and phenolic acids to the cytotoxic action of Russian propolis against murine osteoblasts (MC‐3 T3). Flavonoids seemed also to be involved in the cytotoxic action of a Brazilian propolis sample collected in Bahia State (Mitsui et al, ) and in the Lebanese propolis (Noureddine et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Propolis may exert cytotoxic effects towards tumor cells according to the concentration and time of evaluation Cinegaglia, Bersano, Búfalo, & Sforcin, 2013;Seda Vatansever et al, 2010). (Mitsui et al, 2018) and in the Lebanese propolis (Noureddine et al, 2017).…”

Section: Cell Invasion Assaymentioning

confidence: 99%

The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

Mora

Santiago

Conti

et al. 2018

Phytotherapy Research

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Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH‐DA. Transwell assay was used to evaluate the invasiveness of propolis‐treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.

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Algerian Propolis from Distinct Geographical Locations: Chemical Profiles, Antioxidant Capacity, Cytotoxicity and Inhibition of 5‐Lipoxygenase Product Biosynthesis

Ayad,

Hébert,

Doiron

et al. 2024

Chemistry & Biodiversity

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Propolis was collected from honeybee hives in three geographically distinct Algerian climates and extracts were characterized for composition and bioactivity. Bees were identified as native subspecies using an in‐silico DraI mtDNA COI‐COII test. Over 20 compounds were identified in extracts by LC‐MS. Extracts from the Medea region were more enriched in phenolic content (302 ± 28 mg GAE/g of dry extract) than those from Annaba and Ghardaia regions. Annaba extracts had the highest flavonoid content (1870 ± 385 mg QCE/g of dry extract). Medea extracts presented the highest free‐radical scavenging activity (IC50 = 13.5 µg/mL) using the DPPH radical assay while Ghardaia extracts from the desert region were weak (IC50 > 100 µg/mL). Antioxidant activities measured using AAPH oxidation of linoleic acid were similar in all extracts with IC50 values ranging from 2.9 to 4.9 µg/mL. All extracts were cytotoxic (MTT assay) and proapoptotic (Annexin‐V) against human leukemia cell lines in the low µg/mL range, although the Annaba extract was less active against the Reh cell line. Extracts inhibited cellular 5‐lipoxygenase product biosynthesis with IC50 values ranging from 0.6 to 3.2 µg/ml. Overall, examined propolis extracts exhibited significant biological activity that warrant further characterization in cellular and in vivo models.

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Chemical characterization and cytotoxic activity evaluation of Lebanese propolis

Cited by 39 publications

References 61 publications

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

Insight on Propolis from Mediterranean Countries: Chemical Composition, Biological Activities and Application Fields

The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

Algerian Propolis from Distinct Geographical Locations: Chemical Profiles, Antioxidant Capacity, Cytotoxicity and Inhibition of 5‐Lipoxygenase Product Biosynthesis

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