The content of phenolic compounds (total phenolic compounds, tannins, flavonoids and anthocyanidins) of three species of thyme (Thymus pulegioides, Thymus pannonicus, Thymus praecox) of different origin (Bohemian-Moravian highlands, Křivoklát and Považský Inovec) was determined using spectrophotometric methods of European Pharmacopoeia 8 th edition. Furthermore, the determination of the content of essential oil and analysis of its constituents was realised. The amount of total phenolics was determined by a spectrophotometric method using the Folin-Ciocalteu reagent. Their content ranged from 3.87 to 8.86%. The content of tannins was established on a preliminary determination of the total phenolics, followed by adsorption of tannins on hide powder. The amount of tannins in our samples varied from 1.96 to 5.65%. The content of total flavonoids was determined by a spectrophotometric method using aluminium chloride. Quantitative analysis of flavonoids has shown that content in our samples ranged from 0.59 to 1.52% expressed as luteolin-7-O-glucoside (λ = 392 nm) and from 0.41 to 1.12% expressed as rutin (λ = 420 nm). Anthocyanins represent a small amount of total polyphenol content in Thymus species. In our samples, the content of anthocyanins expressed as cyanidin-3-O-glucoside varied from 0.02 to 0.1%. The determination of essential oil was realised by method of European Pharmacopoeia 8 th edition. Its contents in our samples ranged from 0.2 to 0.75%. Gas chromatography-mass spectrometry was used for essential oil analysis. According to the presence of main monoterpene in Thymus essential oil, we can distinguish three chemotypes: thymol, carvacrol and linalool. Due to variations of chemical constituents of thyme essential oil, it was possible to observe a relationship between occurrence of certain chemotype and origin of sample. Differences in content of flavonoids have not shown a significant relationship to locality of origin.Predložená práca bola zameraná na stanovenie obsahu vybraných fenolových zlúčenín (celkové fenoly, triesloviny, flavonoidy a antokyány) troch rôznych druhov rodu Thymus (T. pulegioides, T. pannonicus, T. praecox) s rozdielnym pôvodom (Českomoravská vrchovina, Křivoklát a Považský Inovec). Na stanovenie týchto zložiek boli využité spektrofotometrické metódy Európskeho liekopisu, 8. vydanie. Zároveň bol stanovený aj obsah silice a bola vykonaná analýza jej zložiek. Obsah celkových polyfenolov bol stanovený spektrofotometrickou metódou s využitím Folin-Ciocalteovho činidla. Obsah kolísal od 3.87 % do 8,86 %. Obsah trieslovín bol realizovaný na základe predošlého stanovenia obsahu celkových fenolov, a to adsorpciou trieslovín na kožný prášok. Ich obsah sa pohyboval v rozmedzí 1,96 % do 5,65 %. Na stanovenie obsahu flavonoidov bola využitá spektrofotometrická metóda s chloridom hlinitým. Obsah flavonoidov vyjadrených ako luteolín-7-O-glukozid (λ = 392 nm) kolísal od 0,59 % do 1,52 % a obsah flavonoidov vyjadrených ako rutín (λ = 420 nm) sa pohyboval v rozmedzí od 0,41 % do 1,12 %. Antokyány zaber...