Chemical characterisation and quantification of the major constituents in the Chinese herbal formula Jian‐Pi‐Yi‐Shen pill by UPLC‐Q‐TOF‐MS/MS and HPLC‐QQQ‐MS/MS

Abstract

Introduction: Jian-Pi-Yi-Shen pill (JPYSP) is a Chinese medicine formula developed for the treatment of anaemic patients with chronic kidney disease (CKD). Objective: To investigate the chemical profile of JPYSP in the treatment of renal anaemia. Methods: A method coupling ultra-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) was established to characterise the chemical constituents present in JPYSP. Subsequently, a high-performance liquid chromatography method coupled with triple-quadrupole tandem mass spectrometry (HPLC-QQQ-MS/MS) was developed to quantify the major constituents from the identified compounds related to the treatment of CKD and anaemia. Results: A total of 71 compounds were tentatively identified from JPYSP, including saponins, flavonoids, sesquiterpenoids, coumarins, phenylpropanoids, anthranones, anthraquinones, tannins, phenolic acids and others. Amongst them, 12 compounds (i.e. astragaloside IV, calycosin, calycosin 7-O-glucoside, salvianolic acid A, rosmarinic acid, rhein, liquiritin, formononetin, atractylenolide I, dioscin, tanshinone IIA, and acteoside) were further quantified simultaneously by HPLC-QQQ-MS/MS. Conclusion: The newly developed approach is suitable for the chemical profiling analysis and quality control of JPYSP, and could lead to additional pharmacodynamic studies involving the components of JPYSP.

Phytochemical Analysis Using UPLC-MS/MS Combined with Network Pharmacology Methods to Explore the Biomarkers for the Quality Control of Lingguizhugan Decoction

“…Because caffeoyl groups often exist in its structure, the characteristic fragment ions of [M-H-C 9 H 6 O 3 ] can often be obtained in the fragmentation of mass spectrometry. In addition, it is easy to lose CH 3 , H 2 O, CO, CO 2 , or COOH to obtain fragment ions with mass loss of 15, 18, 28, 44, and 45 Da [ 19 , 20 ]. For example, compound 2-methoxycinnamic acid(C 10 H 10 O 3 , [M-H] − , P46) produced an m/ z peak at 162.0326 upon the elimination of CH 3 , and this was followed by the loss of COOH groups in succession to give peaks at m/ z 117.0339( Figure 7(a) ).…”

Jian-Pi-Yi-Shen formula enhances perindopril inhibition of chronic kidney disease progression by activation of SIRT3, modulation of mitochondrial dynamics, and antioxidant effects

“…The chemical constituents presented in JPYSF extract have been characterized by using ultra-performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) in our previous study. A total of 71 compounds were tentatively identified from JPYSF extract, including saponins, flavonoids, sesquiterpenoids, coumarins, phenylpropanoids, anthranones, anthraquinones, tannins, phenolic acids, and others [ 18 ].…”

Jian-Pi-Yi-Shen Formula Alleviates Chronic Kidney Disease in Two Rat Models by Modulating QPRT/NAD+/SIRT3/Mitochondrial Dynamics Pathway

“…Following subsequent centrifugation, the supernatant was dried by a freeze dryer and stored at −80°C. The chemical profile and quality control of JPYSF extract have been well described by using ultraperformance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) in our previous study [ 18 ]. Briefly, a total of 71 compounds were identified from JPYSF extract, including saponins, flavonoids, sesquiterpenoids, coumarins, phenylpropanoids, anthranones, anthraquinones, tannins, phenolic acids, and others.…”