Chemical bioimaging for the subcellular localization of trace elements by high contrast TEM, TEM/X-EDS, and NanoSIMS

Penen, Florent; Malherbe, Julien; Isaure, Marie‐Pierre; Dobritzsch, Dirk; Bertalan, Ivo; Gontier, Étienne; Coustumer, Philippe Le; Schaumlöffel, Dirk

doi:10.1016/j.jtemb.2016.04.014

Cited by 36 publications

(32 citation statements)

References 34 publications

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“…Samples were cut in 70 and 300 nm sections for TEM and NanoSIMS, respectively, using a diamond knife (Diatome, Biel‐Bienne, Switzerland) on an ultra‐microtome (EM Ultracut‐UC7, Leica Microsystems) and placed on the respective sample holder. In order to carry out the correlative imaging by TEM and NanoSIMS, a first 70 nm section was placed on a copper grid for TEM and then, an adjacent 300 nm section was placed on a silicon wafer (Wafer Solution, Le Bourget du lac, France) for NanoSIMS, as previously described (Penen et al, ). Cells were localized and observed first by TEM and then, relocalized and analysed by NanoSIMS.…”

Section: Methodsmentioning

confidence: 99%

“…Ion beam and X‐ray beam imaging techniques are powerful tools to study the isotopic and elemental composition of single cells (Ortega, Devès, & Carmona, ; Penen et al, ; Roschzttardtz et al, ; Sarret et al, ; Schaumlöffel et al, ). Nanoscale secondary ion mass spectrometry (NanoSIMS) allows elemental and isotopic mapping with a resolution of about 50 nm.…”

Section: Introductionmentioning

confidence: 99%

“…In biology, it is used to localize and to determine at a subcellular level the turnover of proteins and metabolites using stable isotopic tracers ( 13 C and 15 N) (Gao, Huang, & Tao, 2016;Lechene et al, 2006). Moreover, when NanoSIMS is combined with transmission electron microscopy (TEM) in correlative imaging, isotopic tracers can be localized in the cell structure (Clode, Stern, & Marshall, 2007;Hoppe, Cohen, & Meibom, 2013;Kopp et al, 2015;Penen et al, 2016). NanoSIMS mapping has been also used to determine metal distributions in plants and microalgae (Hong-Hermesdorf et al, 2014;Moore et al, 2010Moore et al, , 2011Moore et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pyrenoidal sequestration of cadmium impairs carbon dioxide fixation in a microalga

Penen

Isaure

Dobritzsch

et al. 2019

Plant Cell & Environment

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Mixotrophic microorganisms are able to use organic carbon as well as inorganic carbon sources and thus, play an essential role in the biogeochemical carbon cycle. In aquatic ecosystems, the alteration of carbon dioxide (CO2) fixation by toxic metals such as cadmium – classified as a priority pollutant – could contribute to the unbalance of the carbon cycle. In consequence, the investigation of cadmium impact on carbon assimilation in mixotrophic microorganisms is of high interest. We exposed the mixotrophic microalga Chlamydomonas reinhardtii to cadmium in a growth medium containing both CO2 and labelled 13C‐[1,2] acetate as carbon sources. We showed that the accumulation of cadmium in the pyrenoid, where it was predominantly bound to sulphur ligands, impaired CO2 fixation to the benefit of acetate assimilation. Transmission electron microscopy (TEM)/X‐ray energy dispersive spectroscopy (X‐EDS) and micro X‐ray fluorescence (μXRF)/micro X‐ray absorption near‐edge structure (μXANES) at Cd LIII‐edge indicated the localization and the speciation of cadmium in the cellular structure. In addition, nanoscale secondary ion mass spectrometry (NanoSIMS) analysis of the 13C/12C ratio in pyrenoid and starch granules revealed the origin of carbon sources. The fraction of carbon in starch originating from CO2 decreased from 73 to 39% during cadmium stress. For the first time, the complementary use of high‐resolution elemental and isotopic imaging techniques allowed relating the impact of cadmium at the subcellular level with carbon assimilation in a mixotrophic microalga.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pyrenoidal sequestration of cadmium impairs carbon dioxide fixation in a microalga

Penen

Isaure

Dobritzsch

et al. 2019

Plant Cell & Environment

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“…One application of the new source was the imaging of trace elements in single cells (Penen et al . ), by combining transmission electron microscopy (TEM) and NanoSIMS. A similar methodology was also developed by Miot et al .…”

Section: Advances In Secondary Ion Mass Spectrometrymentioning

confidence: 99%

GGR Biennial Critical Review: Analytical Developments Since 2014

Linge

Bèdard

Bugoi

et al. 2017

Geostandard Geoanalytic Res

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This GGR biennial critical review covers developments and innovations in key analytical methods published since January 2014, relevant to the chemical, isotopic and crystallographic characterisation of geological and environmental materials. In nine selected analytical fields, publications considered to be of wide significance are summarised, background information is provided and their importance evaluated. In addition to instrumental technologies, this review also presents a summary of new developments in the preparation and characterisation of rock, microanalytical and isotopic reference materials, including a précis of recent changes and revisions to ISO guidelines for reference material characterisation and reporting. Selected reports are provided of isotope ratio analyses by both solution-nebulisation MC-ICP-MS and laser ablation-ICP-MS, as well as of radioactive isotope geochronology by LA-ICP-MS. Most of the analytical techniques elaborated continue to provide new applications for geochemical analysis, however it is noted that instrumental neutron activation analysis has become less popular in recent years, mostly due to the reduced availability of nuclear reactors to act as a neutron source. Many of the newer applications reported here provide analysis at increasingly finer resolution. Examples include atom probe tomography, a very sensitive method providing atomic scale information, nanoscale SIMS, for isotopic imaging of geological and biological samples, and micro-XRF, which has a spatial resolution many orders of magnitude smaller than conventional XRF

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“…The technique is capable of analyzing the sub-micron-scale distribution of Cu, as it has been applied with different environmental samples including, for instance, atmospheric particles, Au-bearing pyrite, biological samples, and aggregates from an arable soil horizon. [12][13][14][15][16][17][18][19] To the best of our knowledge, the small-scale Cu distribution in contaminated technogenic soil has not been studied by NanoSIMS and subsequently evaluated by digital image processing yet. We therefore hypothesized that the combination of NanoSIMS analyses and image processing would help to enlighten the previously unknown sub-micron Cu distribution.…”

Section: Introductionmentioning

confidence: 99%

Small‐scale distribution of copper in a Technosol horizon studied by nano‐scale secondary ion mass spectrometry

Rennert

Höschen

Rogge

et al. 2018

Rapid Comm Mass Spectrometry

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Chemical bioimaging for the subcellular localization of trace elements by high contrast TEM, TEM/X-EDS, and NanoSIMS

Cited by 36 publications

References 34 publications

Pyrenoidal sequestration of cadmium impairs carbon dioxide fixation in a microalga

Pyrenoidal sequestration of cadmium impairs carbon dioxide fixation in a microalga

GGR Biennial Critical Review: Analytical Developments Since 2014

Small‐scale distribution of copper in a Technosol horizon studied by nano‐scale secondary ion mass spectrometry

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