Chemical, antioxidant and cytotoxic profile of hydroalcoholic extracts of plants from Southern Brazil and their activity against pathogenic fungi isolated from dogs and cats with sensitivity and resistance to conventional antifungals

Abstract

We aimed to evaluate the chemical, antioxidant, cytotoxic, and antifungal activities of hydroalcoholic extracts of native plants from Southern Brazil: Schinus terebinthifolia (SCH), Persicaria hydropiperoides (PER), Eugenia uniflora (EUG) and Equisetum hyemale (EQU). Ethyl gallate, quercetin, and quinic acid were prevalent compounds identified by LC-MS. For total phenolic/flavonoid contents and the antioxidant potential against ABTS/DPPH radicals, the ascending order was EQU<PER<EUG<SCH. All extracts were low cytotoxic and kept a high Vero cell viability (>75%) at concentrations up to 12.5 mg/mL (MTT assay). By M38-A2/M27-A3 (CLSI) against 68 clinical isolates of animals and strains of Malassezia pachydermatis, Sporothrix brasiliensis, Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes, all extracts (MIC/MFC ≤3.13-100 mg/mL) were active, except EUG. SCH inhibited and killed S. brasiliensis (MIC/MFC50/90 3.12-12.5 mg/mL) and dermatophytes (MIC/MFC 6.25-25 mg/mL) resistant to ketoconazole and itraconazole. These findings support the promising use of the selected plant extracts as antifungal agents.

Chemical Profile and Biological Potential of Scaptotrigona Bee Products (Hymenoptera, Apidae, Meliponini): An Review

“…These botanical species were previously identified as botanical sources for propolis production by S. bipunctata and other bees of the genus Scaptotrigona. [33][34][35] Dallabona et al [12] also identified for the ethanol extract of S. bipunctata propolis, the presence of volatile compounds that mainly include terpenoids, with emphasis on mono and sesquiterpenoids. The identified compounds of this class for propolis were the monoterpenes 3-carene, α-pinene, sobrerol, 4terpinenol, and sesquiterpenes, such as oplopanone and αnerolidol.…”

Chemical and cytotoxicity profiles of 11 pink pepper (Schinus spp.) samples via non-targeted hyphenated high-performance thin-layer chromatography

“…The bark and fruits are used to treat urinary and uterine affections, bleeding and diarrhea, and rheumatic and respiratory diseases. Antioxidant (Bendaoud et al, 2010 ; Feriani et al, 2021 ; Kim et al, 2021 ; Oliveira et al, 2020b ), antibacterial (D'Sousa' Costa et al, 2015 ; Linden et al, 2020 ; Salem et al, 2018 ), antifungal (Giordani et al, 2022 ), antidiabetic (Dos Santos da Rocha et al 2019 ; Iwanaga et al, 2019 ), and other bioactivities have been reported. Anti-inflammatory properties, for example, have been linked to the composition of polyphenols, as well as monoterpenes and sesquiterpenes in the essential oil (Feriani et al, 2020 ; Formagio et al, 2011 ; Kim et al, 2021 ; Marangoni et al, 2022 ; Rosas et al, 2015 ; Yueqin et al, 2003 ).…”