Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MS/MS, Electron Microscope, and Congo Red Staining

Lu, Chia Ming; Hou, Mei; Lin, Lie Chwen; Tsai, Tung Hu

doi:10.1155/2013/952796

Cited by 14 publications

(23 citation statements)

References 30 publications

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“…The study of bioactive constituents extracted by JWXYS is not clarified thoroughly. A previous study reveals that saikosaponin A and saikosaponin D in Radix Bupleuri (Chai-Hu), ferulic acid in Radix Angelicae Sinensis (Dang-Gui), and paeoniflorin in Radix Paeoniae Alba (Bai-Shao) and Cortex Moutan Radicis (Mu-Dan-Pi) would be extracted in JWXYS [ 39 ]. Furthermore, flavonoids in several herbs and glycyrrhizic acid in Radix Glycyrrhizae Uralensis (Zhi-Gan-Cao) are water-soluble components in JWXYS and could be extracted by water.…”

Section: Resultsmentioning

confidence: 99%

Herb-Drug Pharmacokinetic Interaction of a Traditional Chinese Medicine Jia-Wei-Xiao-Yao-San with 5-Fluorouracil in the Blood and Brain of Rat Using Microdialysis

Chiang¹,

Chang²,

Wang³

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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According to a survey from the National Health Insurance Research Database (NHIRD), Jia-Wei-Xiao-Yao-San (JWXYS) is the most popular Chinese medicine for cancer patients in Taiwan. 5-Fluorouracil (5-FU) is a general anticancer drug for the chemotherapy. To investigate the herb-drug interaction of JWXYS on pharmacokinetics of 5-FU, a microdialysis technique coupled with a high-performance liquid chromatography system was used to monitor 5-FU in rat blood and brain. Rats were divided into four parallel groups, one of which was treated with 5-FU (100 mg/kg, i.v.) alone and the remaining three groups were pretreated with a different dose of JWXYS (600, 1200, or 2400 mg/kg/day for 5 consecutive days) followed by a combination with 5-FU. This study demonstrates that 5-FU with JWXYS (600 mg/kg/day or 1200 mg/kg/day) has no significant effect on the pharmacokinetics of 5-FU in the blood and brain. However, JWXYS (2400 mg/kg/day) coadministered with 5-FU extends the elimination half-life and increases the volume of distribution of 5-FU in the blood. The elimination half-life of 5-FU in the brain for the pretreatment group with 2400 mg/kg/day of JWXYS is significantly longer than that for the group treated with 5-FU alone and also reduces the clearance. This study provides practical dosage information for clinical practice and proves the safety of 5-FU coadministered with JWXYS.

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Section: Resultsmentioning

confidence: 99%

Herb-Drug Pharmacokinetic Interaction of a Traditional Chinese Medicine Jia-Wei-Xiao-Yao-San with 5-Fluorouracil in the Blood and Brain of Rat Using Microdialysis

Chiang¹,

Chang²,

Wang³

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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“…In contrast, modern Chinese medicinal preparations generally use ethanol as a solvent in the extraction process. The chemical compositions of water decoction and ethanol extraction are different Lu et al, 2013;Wang et al, 2013). Compared with the former, ethanol can extract a lot of lipidsoluble constituents such as fatty acid components.…”

Section: Improper Modern Preparation Process Modern Extraction Procesmentioning

confidence: 99%

Polygonum multiflorum-Induced Liver Injury: Clinical Characteristics, Risk Factors, Material Basis, Action Mechanism and Current Challenges

et al. 2019

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Polygonum multiflorum Thunb. (PM), called Heshouwu in China, is a popular Chinese medicine in clinical practice. Several clinical studies have been conducted to evaluate the traditional therapeutic claims and to study the potential therapeutic activity of PM in dyslipidemia and neurodegenerative diseases, highlighting available clinical evidence. In recent years, reports on clinical adverse reactions of Raw Radix P. multiflorum (RPM) and P. multiflorum Praeparata (PMP) have been on the increase, especially with respect to liver injury. Most liver injury cases had been assessed for causality using RUCAM (Roussel Uclaf Causality Assessment Method) in this paper. However, the components of PM responsible for the reported hepatotoxic effects have not yet been identified. Moreover, many of the reports are contradictory, while studies on the mechanism involved in PM-induced liver damage are not comprehensive. This study was aimed at reviewing the status of research on liver injury due to PM, including clinical characteristics, risk factors, material basis research and mechanism of action, with a view to understanding PM-induced hepatotoxicity, and taking reasonable and effective measures to prevent it. In short, quality control is still one of the major safety problems in TCM drug safety concerns. The model of safety monitoring and risk management of PM drugs is not yet developed. Indeed, the characteristics and risk factors associated with PM require both proper understanding and control of the risk by strengthening standardization of clinical applications, basic science research, quality control in manufacturing, active monitoring methodology and enhancement of international communication and cooperation. Measures should also be encouraged and implemented to promote healthy development of the TCM industry.

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“…Congo red has recently been used to identify cellulose fibril content in phytochemistry [ 34 ]. Through noncovalent affinity and synthesizing a red complex, Congo red has a strong interaction with polysaccharides [ 31 ]. An Aperio ScanScope slide scanner was used to confirm and identify cellulose fibers through Congo red staining.…”

Section: Resultsmentioning

confidence: 99%

“…A series of physical examinations were performed to assess the starch and raw herbal content in the pharmaceutical herbal products. Based on previous analysis [ 9 , 31 , 35 ], data analysis can be a rapid and reliable indicator of quality control, and it can be used to determine whether raw herbal powders and starch were added to XSLJZT. The photograph of KI-stained cornstarch was used as a positive control to confirm the normal condition of cornstarch for this study.…”

Section: Resultsmentioning

confidence: 99%

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Identification of a Multicomponent Traditional Herbal Medicine by HPLC–MS and Electron and Light Microscopy

Liu

Cheng²,

Hsieh

et al. 2017

Molecules

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Background: Commercial pharmaceutical herbal products have enabled people to take traditional Chinese medicine (TCM) in a convenient and accessible form. However, the quantity and quality should be additionally inspected. To address the issue, a combination of chemical and physical inspection methods were developed to evaluate the amount of an herbal formula, Xiang-Sha-Liu-Jun-Zi-Tang (XSLJZT), in clinical TCM practice. Methods: A high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS) method with electrospray ionization was developed to measure the herbal biomarkers of guanosine, atractylenolide III, glycyrrhizic acid, dehydrocostus lactone, hesperidin, and oleanolic acid from XSLJZT. Scanning electron microscopy (SEM) photographs and light microscopy photographs with Congo red and iodine–KI staining were used to identify the cellulose fibers and starch content. Furthermore, solubility analysis, swelling power test, and crude fiber analysis were contributed to measure the starch additive in pharmaceutical products. Results: The results demonstrated large variations in the chemical components of different pharmaceutical brands. The SEM photographs revealed that the starch was oval, smooth, and granular, and that the raw herbal powder appears stripy, stretched, and filiform. The stained light microscopy photographs of all of the pharmaceutical products showed added starch and raw herbal powder as extenders. Conclusion: The developed chemical and physical methods provide a standard operating procedure for the quantity control of the herbal pharmaceutical products of XSLJZT.

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Chemical and Physical Methods to Analyze a Multicomponent Traditional Chinese Herbal Prescription Using LC-MS/MS, Electron Microscope, and Congo Red Staining

Cited by 14 publications

References 30 publications

Herb-Drug Pharmacokinetic Interaction of a Traditional Chinese Medicine Jia-Wei-Xiao-Yao-San with 5-Fluorouracil in the Blood and Brain of Rat Using Microdialysis

Herb-Drug Pharmacokinetic Interaction of a Traditional Chinese Medicine Jia-Wei-Xiao-Yao-San with 5-Fluorouracil in the Blood and Brain of Rat Using Microdialysis

Polygonum multiflorum-Induced Liver Injury: Clinical Characteristics, Risk Factors, Material Basis, Action Mechanism and Current Challenges

Identification of a Multicomponent Traditional Herbal Medicine by HPLC–MS and Electron and Light Microscopy

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