Chemical and Pharmacological Investigation of the Stem Bark of Synadenium grantii

Munhoz, Antônio Carlos Mattar; Minozzo, Bruno Rodrigo; Cruz, Luiza Stolz; Oliveira, Thais Latansio de; Machado, Willian Moreira; Pereira, Airton Vicente; Fernandes, Daniel; Manente, Francine Alessandra; Vellosa, José Carlos Rebuglio; Nepel, Angelita; Barison, Andersson; Beltrame, Flávio Luís

doi:10.1055/s-0034-1368300

Cited by 26 publications

(33 citation statements)

References 35 publications

(66 reference statements)

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“…The spectroscopic data compares very well with those of friedelin in literature (Queiroga et al, 2000). It has been previously isolated from Maytenus ilicifolia (Queiroga et al, 2000), Maytenus robusta (Sousa et al, 2012), Terminalia avicennioides (Mann et al, 2011), Synadenium grantii (Munhoz et al, 2014), Azima tetracantha (Antonisamy et al, 2015), Blighia inijuigata (Ongarora et al, 2009), Kielmeyera neglecta (Souza et al, 2012) and Garcinia mangostana (Parveen, 2009). It has however not been previously reported in B. sapida.…”

Section: Larvicidal Assaysupporting

confidence: 75%

Activity of the compounds isolated from Blighia sapida (sapindaceae) stem bark against Aedes aegypti larvae

Famuyiwa¹,

Famuyiwa²,

Aladesanmi³

2018

Ife Journal of Science

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Blighia sapida (Sapindaceae) is commonly known as Ackee and in the South West Nigeria it is called Isin. The wood is resistant to termites and the pounded fruit and the stem bark is used as fish poison. The methanol extract of the stem bark reported to be active against the fourth instar larvae of Aedes aegypti was suspended in water and partitioned into n-hexane, dichloromethane and ethyl acetate. The most active n-hexane fraction with activity that was statistically insignificant to that of the methanolic extract gave LC , LC values of 1.81 ± 0.02, 3.17 ± 50 90 0.02 mg/mL at 24 h and 1.65 ± 0.05, 2.82 ± 0.35 mg/mL at 48 h. Endosulphan the positive control, had LC , 50 LC values of 0.93 ± 0.06, 1.61 ± 0.12 mg/mL at 24 h and 0.90 ± 0.09, 1.44 ± 0.11 mg/mL at 48 h. The n-hexane 90 fraction was subjected to chromatographic separations that led to the isolation of friedelin and α-amyrin. Friedelin and α-amyrin gave LC LC 0.07 ± 0.01, 0.13 ± 0.01 mg/mL and 0.05 ± 0.00, 0.08 ± 0.01 mg/mL 50, 90 respectively at 24 h and at 48 h there was 100% mortality. The activity of both compounds was statistically higher than that of Endosulphan.

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Section: Larvicidal Assaysupporting

confidence: 75%

Activity of the compounds isolated from Blighia sapida (sapindaceae) stem bark against Aedes aegypti larvae

Famuyiwa¹,

Famuyiwa²,

Aladesanmi³

2018

Ife Journal of Science

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“…Phytochemical analysis using TLC, together with standard compounds already isolated from the plant (friedelin, 3b-friedelinol, eufol, and citrostadienol) [9][10][11][12][13][14] indicated that these were absent, suggesting other active principles that could act as antiproliferative agents, like compound 1. (Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…7 Some other compounds have been found in this plant, such as diterpene esters, anthocyanins, and unsaponificable substances. [6][7][8][9][10] As part of the Network Ribecancer 212RT 0464 (CYTED/ CNPq), the aim of this study was to evaluate the antiproliferative activity of the crude methanol extract and fractions/subfractions of the stem of S grantii as well as a rare isolated compound (phorbol diterpene ester) denoted as 3,4,12,13tetraacetylphorbol-20-phenylacetate (compound 1).…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

et al. 2016

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Synadenium grantii is frequently used for the treatment of various diseases such as allergies, gastric disorders, and especially cancer. The aim of this study was to evaluate the possible antiproliferative potential of the methanol extract, fractions, and pure compounds from the stems of S grantii Phytochemical analysis was carried out by conventional chromatographic techniques, and the antiproliferative activity was analyzed using the sulforhodamine B assay and an MTT-based assay. Nonpolar fraction and its subfractions from the stems of S grantii exhibited promising cytostatic effect against several human tumor cell lines (glioma, breast, kidney, and lung), with total grown inhibition values ranging from 0.37 to 2.9 μg/mL. One of the active principles of this plant was identified as a rare phorbol diterpene ester, denoted as 3,4,12,13-tetraacetylphorbol-20-phenylacetate. This compound demonstrated antiproliferative activity against glioma, kidney, lung, and triple-negative breast cancer cell lines. These results demonstrate that S grantii stems produce active principles with relevant antiproliferative potential.

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“…[5,6] Phytochemical investigations demonstrated the presence of proteins, fatty acids, phenolic, and terpene compounds. [7][8][9] Biological studies have shown cytotoxicity activity of the latex, bark and leaves extracts against B16F10 cells (melanoma), HeLa (adenocarcinoma), HRT-18 (ileocecal colorectal adenocarcinoma), Jurkat cells (acute T cell leukemia) and K-562 (chronic myelogenous leukemia). [10 -14] These studies have demonstrated that fractionation improves the cytotoxic activity of the compounds present in these vegetal matrices.…”

Section: Introductionmentioning

confidence: 99%

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Cruz

Kanunfre

Andrade

et al. 2020

Chemistry & Biodiversity

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The current study was carried out by a bioguided fractionation of a hexane extract of the latex of Euphorbia umbellata against leukemic cells. Samples were analyzed by NMR, GC/MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC/MS analysis. MTT reduction assay demonstrated that HL‐60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices were higher than 22.44. Bioguided‐fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.

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Chemical and Pharmacological Investigation of the Stem Bark of Synadenium grantii

Cited by 26 publications

References 35 publications

Activity of the compounds isolated from <i>Blighia sapida</i> (sapindaceae) stem bark against <i>Aedes aegypti</i> larvae

Activity of the compounds isolated from <i>Blighia sapida</i> (sapindaceae) stem bark against <i>Aedes aegypti</i> larvae

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

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