Chemical and Microbiological Determination of Vitamin B
            <sub>6</sub>

Storvick, Clara A.; Benson, Eva M.; Edwards, Margaret A.; Woodring, Muriel J.

doi:10.1002/9780470110300.ch5

Cited by 39 publications

(2 citation statements)

References 138 publications

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“…However, high salt amounts can suppress growth of the medium, and different growth responses for pyridoxine, pyridoxal, and pyridoxamine add to the assay's complexity. Hydrolyzing the samples followed by the chromatographic separation of the vitamers before addition to the yeast basal medium allows the determination of each vitamer individually (13,14,(58)(59)(60)(61)(62). Other microorganisms such as Kloeckera brevis and Lactobacillus casei have also been described (12,63,64), but the extensive sample preparation and complexities of microbiological assays have led to the development of chromatographic methods for vitamin B-6 analysis (9).…”

Section: Vitamin B-6mentioning

confidence: 99%

Micronutrients in Human Milk: Analytical Methods

Hampel

Dror

Allen

2018

Advances in Nutrition

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Exclusive breastfeeding is recommended by the WHO for the first 6 mo of life because human milk protects against gastrointestinal infections and supplies balanced and adequate nutrient contents to the infant. However, reliable data on micronutrient concentrations in human milk are sparse, especially because some micronutrients are affected by maternal diet. Microbiological and competitive protein-binding assays, nuclear magnetic resonance or inductively coupled plasma spectroscopy, and chromatographic analyses are among the methods that have been applied to human-milk micronutrient analysis. However, the validation or evaluation of analytical methods in terms of their suitability for the complex human-milk matrix has been commonly ignored in reports, even though the human-milk matrix differs vastly from blood, plasma, or urine matrixes. Thus, information on the validity, accuracy, and sensitivity of the methods is essential for the estimation of infant and maternal intake requirements to support and maintain adequate milk micronutrient concentrations for healthy infant growth and development. In this review, we summarize current knowledge on methods used for analyzing water- and fat-soluble vitamins as well as iron, copper, zinc, iodine, and selenium in human milk and their different forms in milk; the tools available for quality control and assurance; and guidance for preanalytical considerations. Finally, we recommend preferred methodologic approaches for analysis of specific milk micronutrients.

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Section: Vitamin B-6mentioning

confidence: 99%

Micronutrients in Human Milk: Analytical Methods

Hampel

Dror

Allen

2018

Advances in Nutrition

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“…[Various chemical, enzymatic, and microbiological methods for determining pyridoxal-P and some applications have been compiled (McCormick & Wright, 1970;Storvick * Visiting Biochemist. The reaction was clearly pyridoxal-P dependent (Ressler e t aZ., 1973).…”

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confidence: 99%

Enzymatic Determination of Pyridoxal 5‘‐phosphate With Γ‐cyanoaminobutyric Acid Aposynthase

Abe

Ressler

1979

International Journal of Peptide and Protein Research

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A rapid alternative method is presented for the determination of pyridoxal 5'-phosphate (pyridoxal-P). The method involves the colorimetric analysis o f thiocyanate liberated from S-cyanohomocysteine (Hcy (CN)) in the presence o f cyanide when catalyzed by the pyridoxal-P dependent enzyme, y-cyano-aaminobutyric acid (y-CNabuJ-synthase {Hcy (CN) thiocyano-lyase (adding C N ] ) . The rate of formation of thiocyanate is determined by the increase in absorbance at 470 nm on treatment o f the enzymatic reaction mixture with FeCl3.

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