Chemical- and Irradiation-Induced Mutants and TILLING

Bhat, Ramesh; Upadhyaya, Narayana M.; Chaudhury, Abed; Raghavan, Chitra; Qiu, Fulin; Wang, Hehe; Wu, Jianli; McNally, Kenneth L.; Leung, Hei; Till, B. J.; Henikoff, Steven; Comai, Luca

doi:10.1007/0-387-48914-2_8

Cited by 28 publications

(30 citation statements)

References 81 publications

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“…Use of chemically induced mutants such as ethyl methane sulfonic acid (EMS) has helped to create useful genetic variations in the past (Bhat et al, 2007;Till et al, 2007;Henry et al, 2014;Mohapatra et al, 2014). It has been shown that mainly GC to AT transitions were induced by EMS in Arabidopsis, maize and wheat, (Till et al, 2003;Till et al, 2004;Slade, 2005).…”

Section: Introductionmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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Mutants are powerful genetic resources in plant breeding and functional genomics studies. Sixty seven stable ethyl methane sulphonate (EMS) induced rice mutants and the wild type parent Nagina 22 (N22) were characterized for plant height, tiller number, panicle number and grain yield under normal, low P field and alternate wet and dry (AWD) conditions in the same season. They were also genotyped with 44 SSR markers and four Pup1 (Phosphorus uptake1) gene specific markers. Genetic diversity was analysed by combining phenotype and marker data using Ward-MLM method. Single marker analysis showed significant association of four markers RM19696, RM263, RM3688 and RM1942 with grain yield in all three conditions. K-1, a Pup1 gene specific marker was significantly associated with tiller number only under low P conditions. The average dissimilarity between mutants was 0.86 and cophenetic correlation coefficient was 0.74. Six mutants were selected as gain-of-function mutants as they showed significantly higher grain yield in all three conditions, compared with N22. The selected mutants are an important resource for gene discovery for enhanced tolerance to low P and water stress conditions and associated markers can be useful in marker assisted selection.

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Section: Introductionmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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“…Chemicals induce mainly point mutations, and are thus ideal for producing missense and nonsense mutations, which would provide a series of change-of-function mutations. On the other hand, ionizing radiations normally induce chromosomal rearrangements and deletions [1].…”

Section: Introductionmentioning

confidence: 99%

Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination

Talebi¹,

Talebi²,

Shahrokhifar³

2012

AJPS

137

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Chemical and physical mutagenesis has been used to increase genetic variability in crop plants. More than 430 new varieties have been derived as mutants of rice (Oryza sativa L.) via the application of different mutagenic agents. Chemical mutagens such as ethyl methane sulphonate (EMS), diepoxybutane-derived (DEB), sodium azide and irradiation (Gamma rays, X-rays and fast neutrons) have been widely used to induce a large number of functional variations in rice and others crops. Among chemical mutagens, the alkylating agent, ethyl methane sulfonate (EMS) is the most commonly used in plants as it causes a high frequency of nucleotide substitutions, as detected in different genomes. In this study, seeds of potential genotype of the popular variety, (Oryza sativa L. spp. Indica cv. MR219) were treated with EMS at concentrations of 0.25%, 0.50%, 0.75%, 1%, 1.25%, 1.5% and 2%. Sensitivity to EMS was determined by various measurements on the M1 generation. As concentration of applied EMS increased, will decrease in germination, seedling height, root length and emergence under field conditions was observed in M1 generation as compared to the non-treatment control. Plant height and root length also decreased with increases in EMS mutagenesis in an approximately linear fashion. The LD25 and LD50 values were observed based on growth reduction of seedlings after EMS treatment with 0.25% and 0.50% on the rice variety (Oryza sativa L. spp. Indica cv. MR219).

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“…Ionizing radiations normally causes chromosomal rearrangements and deletions. (Bhat et al, 2007). Gamma rays are electromagnetic radiations having shorter wavelength than X rays with more energy and penetrating power.…”

Section: Introductionmentioning

confidence: 99%

“…(Broertijes and Harten, 1988). EMS alkylates are guanine bases and leads to mispairing-alkylated G pairs with T instead of C, resulting in primarily G/C to A/T transitions (Bhat et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of Physical and Chemical Mutagens on Morphological Characters in M1V2 Generation of Tuberose (Polianthes tuberosa L.)

Kannan¹,

Ganga²

2017

Int.J.Curr.Microbiol.App.Sci

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Chemical- and Irradiation-Induced Mutants and TILLING

Cited by 28 publications

References 81 publications

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination

Effect of Physical and Chemical Mutagens on Morphological Characters in M1V2 Generation of Tuberose (Polianthes tuberosa L.)

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