Chemical and Biological Transformation of the Fungicide Vinclozolin

Mercadier, Christine; Véga, Danielle; Bastide, J.

doi:10.1021/jf980275m

Cited by 14 publications

(14 citation statements)

References 20 publications

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“…Moreover, those rates did not monotonically decrease as a function of exposure concentration. This can be explained by the fact that effects are due to the simultaneous occurrence of VZ and its M1 and M2 metabolites which do not show production and elimination kinetics that are directly linked with VZ concentration Mercadier et al 1998). Previous experiments showed that, after 48 h, M1 concentration in exposure water was three times higher than VZ concentration, whereas M2 concentration was four times lower.…”

Section: Discussionmentioning

confidence: 96%

Development of partial life-cycle experiments to assess the effects of endocrine disruptors on the freshwater gastropod Lymnaea stagnalis: a case-study with vinclozolin

Ducrot

Teixeira-Alves

Lopes³

et al. 2010

Ecotoxicology

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Long-term effects of endocrine disruptors (EDs) on aquatic invertebrates remain difficult to assess, mainly due to the lack of appropriate sensitive toxicity test methods and relevant data analysis procedures. This study aimed at identifying windows of sensitivity to EDs along the life-cycle of the freshwater snail Lymnaea stagnalis, a candidate species for the development of forthcoming test guidelines. Juveniles, sub-adults, young adults and adults were exposed for 21 days to the fungicide vinclozolin (VZ). Survival, growth, onset of reproduction, fertility and fecundity were monitored weekly. Data were analyzed using standard statistical analysis procedures and mixed-effect models. No deleterious effect on survival and growth occurred in snails exposed to VZ at environmentally relevant concentrations. A significant impairment of the male function occurred in young adults, leading to infertility at concentrations exceeding 0.025 μg/L. Furthermore, fecundity was impaired in adults exposed to concentrations exceeding 25 μg/L. Biological responses depended on VZ concentration, exposure duration and on their interaction, leading to complex response patterns. The use of a standard statistical approach to analyze those data led to underestimation of VZ effects on reproduction, whereas effects could reliably be analyzed by mixed-effect models. L. stagnalis may be among the most sensitive invertebrate species to VZ, a 21-day reproduction test allowing the detection of deleterious effects at environmentally relevant concentrations of the fungicide. These results thus reinforce the relevance of L. stagnalis as a good candidate species for the development of guidelines devoted to the risk assessment of EDs.

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Section: Discussionmentioning

confidence: 96%

Development of partial life-cycle experiments to assess the effects of endocrine disruptors on the freshwater gastropod Lymnaea stagnalis: a case-study with vinclozolin

Ducrot

Teixeira-Alves

Lopes³

et al. 2010

Ecotoxicology

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“…It's a reason that pesticide degradation by bacteria and fungi has received much attention (Cain and Mitchell 1996;Mercadier et al 1998;Pai et al 2001;Rama and Ligy 2009). A number of bacterial species are known to degrade benzimidazole structures and most of them were isolated from fungicide contaminated soils.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

2010

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Degradation of the fungicide thiophanate-methyl (TM) by Enterobacter sp. TDS-1 and Bacillus sp. TDS-2 isolated from sandy soil previously treated with TM was studied in mineral salt medium (MSM) and soil. Both strains were able to grow in MSM supplemented with TM (50 mg l(-1)) as the sole carbon source. Over a 16 days incubation period, 60 and 77% of the initial dose of TM were degraded by strains TDS-1 and TDS-2, respectively, and disappearance of TM was described by first-order kinetics. Medium supplementation with glucose markedly stimulated bacterial growth; while the final rate of TM degradation was reduced by 21 and 27% for strains TDS-1 and TDS-2, respectively as compared to medium with TM only. Moreover, this additional carbon source changed the TM degradation kinetics, which proceeded according to a zero-order model. This effect was linked to substrate competition and/or a strong decrease of medium pH. Isolates degraded TM (100 mg kg(-1)) in soil with rate constants of 0.186 and 0.210 day(-1), following first-order rate kinetics, and the time in which the initial TM concentration was reduced by 50% (DT50) in soils inoculated with strains TDS-1 and TDS-2 were 6.3 and 5.1 days, respectively. Analysis of TM degradation products in soil showed that the tested strains may have the potential to transform carbendazim (MBC) to 2-aminobenzimidazole (2-AB), and may be useful for a bioremediation of MBC-polluted soils.

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“…The addition of degrading microorganisms to soil can increase pesticide transformation rates [20,21]. B. cepacia introduced into sterile soil quickly degrades methyl benzoate to benzoic acid, which itself is also degraded.…”

Section: Discussionmentioning

confidence: 99%

Microbial hydrolysis of methyl aromatic esters by Burkholderia cepacia isolated from soil

Philippe

Véga

Bastide

2001

FEMS Microbiology Ecology

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A bacterial strain that could utilise methyl benzoate as the sole source of carbon and energy was isolated from soil. This strain was identified as a Burkholderia cepacia. In minimum mineral medium, the strain hydrolysed the methyl ester to form benzoic acid, which is then also degraded. This strain was also able to hydrolyse the ester bond of substituted chlorobenzoic esters and methyl thiophene-2-carboxylate, but did not metabolise their reaction products. A crude enzymatic extract obtained from this strain was relatively stable, and hydrolysed more compounds than the microorganism itself: for instance, methyl 3-(N,N-diethylsulfamoyl)thiophene-2-carboxylate was hydrolysed by the enzyme but not by the microorganism. The bacterial strain was unable to hydrolyse the ester bond of two sulfonylurea herbicides, thifensulfuron methyl and metsulfuron methyl, in solution or after reintroduction in sterile soil. ß

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Chemical and Biological Transformation of the Fungicide Vinclozolin

Cited by 14 publications

References 20 publications

Development of partial life-cycle experiments to assess the effects of endocrine disruptors on the freshwater gastropod Lymnaea stagnalis: a case-study with vinclozolin

Development of partial life-cycle experiments to assess the effects of endocrine disruptors on the freshwater gastropod Lymnaea stagnalis: a case-study with vinclozolin

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

Microbial hydrolysis of methyl aromatic esters by Burkholderia cepacia isolated from soil

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