Chemical and biological investigation of the Agaricus silvaticus Schaeff ex. Secr

Abstract

Extract of Agaricus silvaticus Schaeff ex. Secr fungus have been known to have biological activities, including antibacterial, antiviral, anticancer, antioxidant, anticomplementary and immunostimulating effects. In this study we first time identified that Agaricus silvaticus Schaeff ex. Secr has interferon like activity. We first time isolated the four known compounds such as, mannitol (1), urea (2), ergosterol (3) and ergosterol peroxide (4) from 95% ethanol extracts of Agaricus silvaticus Schaeff ex. Secr in Mongolia. Their structures were elucidated on the basis of extensive analysis of spectral data and by comparison with authentic samples.

Research Advances in Secondary Metabolites and Biological Activities of Mushroom Genus Fungi

“…In 2007, Petrova et al isolated ergosterol peroxide for the first time from A. placomyces and A. pseudopratensis substrates and detected the presence of ergosterol in A. placomyces using GC-MS [1].In 2011, Munkhgerel et al first isolated ergosterol and ergosterol peroxide from A. silvaticus Schaeff ex. Secr [2], and ergosterol peroxide was reported in the literature to have strong antitumour activity and anticomplement activity [3].In 2011, Ueguchi et al isolated ergosterol and ergosterol peroxide from the A. blazei subspecies to isolate three new compounds, among which benzoylergostane showed strong cytotoxicity (IC 50 =6.0±0.33 μmol/L) against HepG2 cells [4] . In 2016, Shimiz et al isolated an ergosterol derivative Agarol from A. blazei subspecies, which had strong cytotoxicity (IC 50 =6.0±0.33 μmol/L) against human cancer cells A549, MKN45, HSC-3 and HSC-4 with significant cytotoxicity and IC 50 of 0.26 µg/mL, 0.34 µg/mL, 1.72 µg/mL and 1.94 µg/mL, respectively, and revealed the molecular mechanism by which the compound Agarol induces apoptosis in cancer cells [5] .…”

“…mycelium, which showed moderate antimicrobial activity against Bacillus subtilis ATCC6633, with 200 mg of the compound per 9 mm agar pore producing a zone of inhibition with a diameter of 16 mm [20].In 2011, Munkhgerel et al urea was isolated from A. silvaticus Schaeff ex. Secr substrate [2].In 2011, Ueguchi et al found N-benzoyl-L-leucine methyl ester from A. blazei substrate, which is the first time that this compound was isolated from nature [4]. ISSN 2616-5910 Vol.…”