Chemical and biological effects of iodination on human albumin

Rosa, U.; Pennisi, F.; Bianchi, R.; Federighi, Giuseppe; Donato, L.

doi:10.1016/0005-2795(67)90553-3

Cited by 62 publications

(10 citation statements)

References 12 publications

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“…13 Radioiodinated tracer batches were eluted from free 125 I through passage in Sephadex G 25 mol/L columns (Column PD-10; Pharmacia), a purification step that reduces free 125 I content in the injected dose to Ͻ1%; repeatability studies had also shown a mean 8.3% intrasubject variation coefficient of TER alb determination. 12 At variance with the original studies performed in the morning after overnight fasts, 12 we decided for technical reasons to run these studies between 2 and 4 PM after the subjects underwent a 4-hour fast, with no tea, coffee, alcohol, or tobacco since early morning.…”

Section: Methodsmentioning

confidence: 99%

Microalbuminuria and Transcapillary Albumin Leakage in Essential Hypertension

et al. 1999

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Abstract-Microalbuminuria (an increased urinary albumin excretion that is not detectable by the usual dipstick methods for macroproteinuria) predicts cardiovascular events in essential hypertensive patients. A possible reason for this behavior is that albumin leaks through exaggeratedly permeant glomeruli exposed to the damaging impact of subclinical atherogenesis. To evaluate this possibility, the transcapillary escape rate of albumin (TER alb , the 1-hour decline rate of intravenous 125 I-albumin), a parameter that estimates the integrity of systemic capillary permeability, albuminuria, blood pressure, echocardiographic left ventricular mass, lipids, and body mass index were measured in 73 uncomplicated, glucose-tolerant men with essential hypertension and normal renal function; 53 were normoalbuminuric, and 20 were microalbuminuric. Twenty-one normotensive age-matched male subjects were the controls. TER alb was higher in hypertensives, a behavior explained in part by a positive correlation with blood pressure values, although body mass index, lipids, and left ventricular mass showed no association. Transcapillary albumin leakage values did not differ between normoalbuminuric and microalbuminuric patients and were unrelated to albuminuria. Blood pressure, particularly systolic, and cardiac mass were higher in microalbuminuric patients in whom albuminuria correlated with both cardiovascular variables and indicated the influence of the hemodynamic load on urinary albumin levels. Thus, TER alb , a parameter influenced by the permeability surface area product for macromolecules and the filtration power across the vascular wall, is altered in essential hypertensives. However, this abnormality is dissociated from the amount of albuminuria, which is contrary to the hypothesis that a higher albumin excretion reflects a greater degree of systemic microvascular damage in essential hypertension. (Hypertension. 1999;34:491-495.) Key Words: albuminuria Ⅲ hypertension, essential Ⅲ cardiac mass Ⅲ blood pressure Ⅲ capillary permeability M icroalbuminuria (ie, an abnormal urinary albumin excretion [UAE] in a range not detectable by the usual dipstick methods for urinary protein) is associated with a greater incidence of cardiovascular events in hypertensive patients 1-3 and predicts all-cause and coronary disease morbidity and mortality independently from other cardiovascular risk factors in nondiabetic populations. 4 -7 However, the reasons why a renal parameter behaves as a marker of atherosclerotic cardiovascular disease in hypertension remain obscure. One concept postulates that more albumin leaks through exaggeratedly permeant glomeruli that reflect the systemic damaging impact of subclinical atherogenesis, 8 a process characterized by a diffuse involvement of the entire vascular system. 9 This hypothesis, which was originally formulated to account for the higher cardiovascular morbidity rate in diabetic patients, 8 may also apply to essential hypertensive patients, 10 but no information is available on this issue at th...

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Section: Methodsmentioning

confidence: 99%

Microalbuminuria and Transcapillary Albumin Leakage in Essential Hypertension

et al. 1999

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“…Amylase was electrolytically iodinated using a technique and apparatus similar to that described by Rosa et al (7). The basic iodination solution contained 0.05 M phosphate buffer (pH 7.4) and 0.10 M sodium chloride.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous study of the metabolic turnover and renal excretion of salivary amylase-125I and pancreatic amylase-131I in the baboon

Duane¹,

Frerichs²,

Levitt³

1972

J. Clin. Invest.

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A B S T R A C T The metabolic turnover of salivary and pancreatic amylase was studied in the baboon, an animal with a serum amylase level and renal clearance of amylase similar to man. Purified amylase was electrolytically iodinated. Although iodinated and uniodinated amylase had similar gel filtration, electrophoretic, enzymatic, glycogen precipitation characteristics, the labeled enzyme was cleared less rapidly by the kidney than was the unlabeled material. However, urinary iodinated amylase which had been biologically screened by the kidney had a renal clearance and serum disappearance rate indistinguishable from unlabeled amylase and thus can serve as a tracer. in metabolic turnover studies. Administration of a mixture of salivary amylase-'I and pancreatic amylase-1I made it possible to simultaneously measure the serum disappearance and renal clearance of these two isoenzymes. The metabolic clearance of both isoenzymes was extremely rapid with half-times of about 130 min. This rapid turnover of serum amylase probably accounts for the transient nature of serum amylase elevation which frequently occurs in pancreatitis.Pancreatic amylase-"I was consistently cleared more rapidly (mean clearance ratio: 1.8) by the kidney than was salivary amylase-'I. This more rapid renal clearance of pancreatic amylase may help to explain the disproportionate elevation of urinary amylase relative to serum amylase observed in pancreatitis.

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“…Rat IgG was radioactively labelled with 125I by the electrolytic method of Rosa, Pennisi, Fedrighi & Donato (1967). The procedure followed has been described in detail elsewhere (Morris & Morris, 1974b).…”

Section: Preparation Of Labelled 8amplesmentioning

confidence: 99%

The effects of corticosterone and cortisone on the uptake of polyvinyl pyrrolidone and the transmission of immunoglobulin G by the small intestine in young rats.

Morris¹,

Morris²

1976

The Journal of Physiology

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1. The distribution of polyvinyl pyrrolidone along the intestinal lumen and in the intestinal wall, following oral administration to normal and corticosterone treated rats, was found to be extremely variable. Valid comparisons between the two groups of animals could not be made using this technique. 2. Three, 4 and 5 days after corticosterone treatment there was no significant change in the uptake of 125I‐labelled polyvinyl pyrrolidone from standard doses injected into ligated segments of the distal small intestine; nor did the treatment induce precocious replacement of the absorptive cells in this region. Cortisone induced precocious cell replacement, a process which took up to 4 days to complete, and also led to a marked reduction in the uptake of 125I‐labelled polyvinyl pyrrolidone from ligated segments of the distal intestine. 3. Three days after treatment with corticosterone (5 mg I.P. at 12 days) there was a marked reduction of labelled immunoglobulin G transport into the blood. Four and 5 days after treatment there was some recovery of the immunoglobulin G transport function. Three days after treatment with cortisone (5 mg I.P. at 12 days) there was closure of the gut to labelled immunoglobulin G. 4. The relevance of these results to antibody transmission and the termination of immunoglobulin transport is discussed.

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Chemical and biological effects of iodination on human albumin

Cited by 62 publications

References 12 publications

Microalbuminuria and Transcapillary Albumin Leakage in Essential Hypertension

Microalbuminuria and Transcapillary Albumin Leakage in Essential Hypertension

Simultaneous study of the metabolic turnover and renal excretion of salivary amylase-125I and pancreatic amylase-131I in the baboon

The effects of corticosterone and cortisone on the uptake of polyvinyl pyrrolidone and the transmission of immunoglobulin G by the small intestine in young rats.

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