Chemical and Biological Analyses of Malaysian Stingless Bee Propolis Extracts

Ibrahim, Nurhamizah; Farah, S. M. N. Nurul; Muslim, M. R. Muhammad; Zakaria, Abd Jamil; Ismail, Zhari; Mohd, Khamsah Suryati

doi:10.17576/mjas-2016-2002-26

Cited by 55 publications

(40 citation statements)

References 17 publications

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“…Therefore, ABTS + radical scavenging assay was selected to analyse the antioxidant activity of EEP since ABTS + has a rapid kinetic reaction and intense response to antioxidants [ 42 ]. As suggested by Ibrahim et al [ 5 ], the increment of antioxidant activity can be observed in the increase of radical scavenging activity that corresponded to a lesser value of IC 50 of the extract. This is in agreement with our present study, where the EEP of T. apicalis possess inhibitory activity against ABTS + radical with IC 50 of 1.68 mg/mL although positive control (Trolox) was better with IC 50 of 0.31 mg/mL.…”

Section: Discussionmentioning

confidence: 61%

“…In Malaysia, multiple researches have been conducted to observe the effectiveness of propolis for some biological activities. The detection of phytochemical compounds and antioxidant activity of Malaysian propolis was successfully discussed in several studies using gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC) [ 5 – 9 ].…”

Section: Introductionmentioning

confidence: 99%

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GC‐MS Evaluation, Antioxidant Content, and Cytotoxic Activity of Propolis Extract from Peninsular Malaysian Stingless Bees, Tetrigona Apicalis

Mohamed

Ismail

Omar

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Introduction. Propolis has been used traditionally in several countries for treating various diseases as it possessed healing properties including antioxidant and anticancer qualities. In Peninsular Malaysia, Tetrigona apicalis is one of the species of stingless bees mainly found in virgin jungle reserves which largely contribute to propolis production. Therefore, this study is designed to evaluate the phytochemical contents, antioxidant properties, and the cytotoxic effect of ethanolic crude of propolis extract against MCF7 and MCF 10A cell lines. Method. The ethanolic extract of propolis (EEP) was extracted using 80% ethanol. Identification of phytochemical contents and antioxidant properties of EEP was analysed by gas chromatography-mass spectrometry (GC-MS) and using 2, 2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) method, respectively. The EEP cytotoxic activity was evaluated on MCF7 and MCF 10A using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Results. Phytochemical contents of EEP demonstrated 28 compounds in which caryophyllene (99%), β-amyrin (96%), α-amyrin (93%), and caryophyllene oxide (93%) were the main compounds. The percentage of ABTS+ scavenging activity of EEP showed an inhibition of 9.5% with half-inhibitory concentration (IC50) value of 1.68 mg/mL. The EEP reduced MCF7 cells viability at IC50 value of 62.24 μg/mL, 44.15 μg/mL, and 32.70 μg/mL at 24, 48, and 72 hours, respectively. The IC50 value of MCF 10A was 49.55 μg/mL, 56.05 μg/mL, and 72.10 μg/mL at 24, 48, and 72 hours, respectively. The EEP cytotoxic effect of T. apicalis was more selective towards MCF7 at 72-hour incubation with a selectivity index (SI) of 2.20. Conclusion. The EEP has been shown to have antioxidants and potential bioactive compounds and inhibited proliferation of the MCF7 cells. Further studies on the EEP role in the apoptosis pathway and its screening towards other cell lines will be evaluated.

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Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

GC‐MS Evaluation, Antioxidant Content, and Cytotoxic Activity of Propolis Extract from Peninsular Malaysian Stingless Bees, Tetrigona Apicalis

Mohamed

Ismail

Omar

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…In Malaysia, research on propolis is limited and until now there are only a few preliminary investigations on the composition and biological activity of propolis derived from Malaysian stingless bees [18]. This study was aimed in analyzing the phytochemical profile of T. apicalis propolis and its antioxidant activities.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

Rosli¹,

Roslan²,

Omar³

et al. 2016

AIP Conference Proceedings

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Abstract. Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyze the phytochemical profile of propolis derived from local T. apicalis species and its antioxidant activities. The ethanolic extract of propolis was subjected to HPLC analysis to analyze its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH radical scavenging assay. TPC and TFC were performed to determine the correlation with its antioxidant activities. TEAC for each serial dilution sample was 2621.15 (4.76 mg/mL), 2050.85 (2.38 mg/mL), 1883.27 (1.19 mg/mL), 1562.67 (0.59 mg/mL), 1327.82 (0.29 mg/mL), 1164.49 (0.15 mg/mL), 983.27 (0.07 mg/mL), and 944.79 (0.04 mg/mL). The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 of propolis for DPPH assay was 4.27 mg/ml. Correlation values of TPC and TFC against DPPH indicate that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid contents. These findings highlighted the importance of quality analysis in order to ensure the consistency of biological effects or therapy of a natural product, such as propolis.

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“…The diverse biological properties and wide application of geopropolis in modern medicine have meant increasing attention has been paid to the identification of new sources of geopropolis and to the study of their chemical composition. Recently, a form of geopropolis produced by stingless bees ( Heterotrigona itama ) and collected in the state of Sarawak, Malaysia, has been shown to exhibit antibacterial activity as well as antioxidant, nitric oxide scavenging, and antidiabetic activities [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…The chemical constituents of this geopropolis have been tentatively studied based on thin layer chromatography and color reactions [ 11 , 12 ]. The results showed that its methanol extract was composed of terpenoids, flavonoids, phenols, steroids, saponin, and coumarins.…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Major Compounds in the Ethanol Extract of Geopropolis from Malaysian Stingless Bees, Heterotrigona itama, by UHPLC-Q-TOF/MS and NMR

LingLing

Sun

et al. 2017

Molecules

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A reliable, rapid analytical method was established for the characterization of constituents of the ethanol extract of geopropolis (EEGP) produced by Malaysian stingless bees—Heterotrigona itama—by combining ultra-high-performance liquid chromatography with quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Based on known standards, the online METLIN database, and published literature, 28 compounds were confirmed. Phenolic acids, flavones, triterpenes and phytosterol were identified or tentatively identified using characteristic diagnostic fragment ions. The results indicated that terpenoids were the main components of EEGP, accompanied by low levels of phenolic acids, flavonoids, and phytosterol. Two major components were further purified by preparative high-performance liquid chromatography (PHPLC) and identified by nuclear magnetic resonance (NMR) as 24(E)-cycloart-24-ene-26-ol-3-one and 20-hydroxy-24-dammaren-3-one. These two triterpenes, confirmed in this geopropolis for the first time, are potential chemical markers for the identification of geopropolis from Malaysian stingless bees, H. itama.

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Chemical and Biological Analyses of Malaysian Stingless Bee Propolis Extracts

Cited by 55 publications

References 17 publications

GC‐MS Evaluation, Antioxidant Content, and Cytotoxic Activity of Propolis Extract from Peninsular Malaysian Stingless Bees, Tetrigona Apicalis

GC‐MS Evaluation, Antioxidant Content, and Cytotoxic Activity of Propolis Extract from Peninsular Malaysian Stingless Bees, Tetrigona Apicalis

Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

Rapid Determination of Major Compounds in the Ethanol Extract of Geopropolis from Malaysian Stingless Bees, Heterotrigona itama, by UHPLC-Q-TOF/MS and NMR

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