Chemical and Biochemical Hydrolysis of Persian Sturgeon (Acipenser persicus) Visceral Protein

Abstract

Chemical (pH 3.3, 70°C, 85°C; pH 12, 70°C, 85°C) and biochemical (Alcalase, Protamex, Neutrase, Flavourzyme, and Trypsin) hydrolysis of Persian sturgeon (Acipenser persicus) visceral protein was investigated. The results of this study revealed that there are significant differences between enzymes in terms of degree of hydrolysis (DH%; P < 0.05). Alcalase-hydrolyzed fish protein had the highest DH% (50.13%), and Trypsinhydrolyzed fish protein had the minimum DH% (14.21%). The highest DH% in chemical hydrolysis was related to pH 3.3 at 85°C (68.87%). The highest protein recovery (83.64%) and protein content (73.34) were related to enzymatic hydrolysis by Alcalase. The results of current study showed the significant effect of hydrolysis conditions on fish protein hydrolysate properties. Microbial enzymes could produce fish hydrolysates with higher degree of hydrolysis when compared to animal enzyme. Also, in chemical hydrolysis it is clear that hydrolysis at the lower pH and at higher temperature causes to more protein recovery and degree of hydrolysis.

Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease

“…In contrast, the investigators [32, 39, 40] had reported a decrease in the DH with prolonged incubation periods. Guerard et al [39] proposed the reduced DH observed with a prolonged incubation period might be caused by limited enzyme activity by the formation of reaction products at a high DH, the decreased concentration of peptide bonds available for hydrolysis, enzyme inhibition, and enzyme deactivation.…”

Optimization of Enzymatic Hydrolysis of Visceral Waste Proteins of Yellowfin Tuna (Thunnus albacares)

“…Generally, Alcalase® 2.4-L-assisted reactions have been repeatedly favored for fish hydrolysis, due to the high degree of hydrolysis that can be achieved in a relatively short time under moderate pH conditions, compared with the neutral or acidic enzymes (Kristinsson and Rasco 2000a, b;Hoyle and Merritt 1994;Shahidi et al 1995;Benjakul and Morrissey 1997;Aspmo et al 2005;Ovissipour et al 2009b).…”

Separation of Iron-Binding Peptides from Shrimp Processing By-products Hydrolysates

“…A number of peptides with significant biological activities have been identified in the enzymatic hydrolysis various marine tissue proteins, for example, antihypertensive activity peptides (Byun and Kim 2001;Qian et al 2007;Lee et al 2010;Tsai et al 2008;Zhao et al 2009;Lee and Song 2009;Sheih et al 2009), antioxidant peptides (Ovissipour et al 2009;Jung et al 2005b;Kim et al 2007a;Je et al 2008;Slizyte et al 2009;Cho et al 2008;Qian et al 2008), anticoagulant peptides (Jo et al 2008;Jung and Kim 2009;Jung et al 2002), antimicrobial peptides (Liu et al 2008;Battison et al 2008;Li et al 2008), angiotensin I converting enzyme (ACE)-inhibitory peptides (Kong et al 2009;He et al 2006;Cheung and Li-Chan 2010;), and mineral carrier peptides (Jung et al 2006;Jung and Kim 2007).…”