Chemical Analysis of Urinary Cotinine in Subjects Exposed to A Mixture of Contaminants

Neamtiu, Iulia A.; Dumitrascu, Irina; Roba, Carmen

doi:10.15373/2249555x/mar2014/18

Cited by 4 publications

(5 citation statements)

References 4 publications

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“…Cotinine was extracted with dichloromethane, evaporated to dryness, dissolved in toluene, and analyzed on a gas chromatograph Shimadzu GS (Shimadzu, Kyoto, Japan) in SIM mode, coupled to a Shimadzu GC-MS TQ8040 mass spectrometer (Shimadzu, Kyoto, Japan) and a Shimadzu AOC 20i autosampler (Shimadzu, Kyoto, Japan). Further details about the method can be found in our previous publications [ 42 , 45 ].…”

Section: Methodsmentioning

confidence: 99%

Arsenic Methyltransferase and Apolipoprotein E Polymorphism in Pregnant Women Exposed to Inorganic Arsenic in Drinking Water in Western Romania

Pop,

Berindan-Neagoe,

Bloom

et al. 2024

IJMS

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Previous studies have shown that inorganic arsenic (iAs) exposure may be associated with genotoxic and cytotoxic effects. The aim of this study was to evaluate the relationship between several polymorphisms in AS3MT and APOE genes and urinary As and the relationship between these polymorphisms and pregnancy loss. We determined urinary As concentrations and performed genotyping analysis in 50 cases of spontaneous pregnancy loss and 50 controls, matched to cases on gestational age. The most frequently identified AS3MT polymorphisms in both cases and controls were in rs10748835 (80% cases and 68% controls), rs3740400 (78% cases and 64% controls), rs7085104 (74% cases and 48% controls), and rs1046778 (62% cases and 54% controls). We identified 30 different haplotypes in AS3MT SNPs, with four predominant haplotypes (>8%). Cases with Haplotype 1 had four-fold higher urinary DMA and two-fold higher MMA concentration than those without this haplotype, the MMA levels were lower in cases and controls with Haplotype 4 compared to Haplotype 1, and the DMA levels were significantly lower in cases with Haplotype 4 compared to Haplotype 3. Cases with Haplotype 1 had higher levels of all analyzed biomarkers, suggesting that Haplotype 1 may be associated with greater exposure to iAs and tobacco smoke. Our results suggest the importance of the AS3MT gene in iAs metabolism among pregnant women with low-level drinking water iAs exposure.

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Section: Methodsmentioning

confidence: 99%

Arsenic Methyltransferase and Apolipoprotein E Polymorphism in Pregnant Women Exposed to Inorganic Arsenic in Drinking Water in Western Romania

Pop,

Berindan-Neagoe,

Bloom

et al. 2024

IJMS

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“…Within 15 min of urine collection, samples were frozen at –20°C and then transferred to the Environmental Health Center (Cluj-Napoca, Romania), where they were stored until analysis for cotinine, arsenic, and five phthalate monoesters. Urinary creatinine was measured according to a previously described procedure, ( Neamtiu, Dumitrascu & Roba, 2014 ), for which the intra-assay coefficient of variation (CV) was 6.5%.…”

Section: Methodsmentioning

confidence: 99%

“…The analytic method for determination of urinary cotinine was previously described in detail ( Neamtiu, Dumitrascu & Roba, 2014 ). In brief, cotinine was extracted with dichloromethane, dissolved in toluene, and analyzed using a QP 2010 Plus NCI gas chromatograph (Shimadzu, Japan) coupled to a mass spectrometer (GC-MS) operated in the selective ion monitoring mode.…”

Section: Methodsmentioning

confidence: 99%

Impact of exposure to tobacco smoke, arsenic, and phthalates on locally advanced cervical cancer treatment—preliminary results

Neamtiu

Bloom

Dumitrascu

et al. 2016

PeerJ

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BackgroundCancer research is a national and international priority, with the efficiency and effectiveness of current anti-tumor therapies being one of the major challenges with which physicians are faced.ObjectiveTo assess the impact of exposure to tobacco smoke, arsenic, and phthalates on cervical cancer treatment.MethodsWe investigated 37 patients with locally advanced cervical carcinoma who underwent chemotherapy and radiotherapy. We determined cotinine and five phthalate metabolites in urine samples collected prior to cancer treatment, by gas chromatography coupled to mass spectrometry, and urinary total arsenic by atomic absorption spectrometry with hydride generation. We used linear regression to evaluate the effects of cotinine, arsenic, and phthalates on the change in tumor size after treatment, adjusted for confounding variables.ResultsWe detected no significant associations between urinary cotinine, arsenic, or phthalate monoesters on change in tumor size after treatment, adjusted for urine creatinine, age, baseline tumor size, and cotinine (for arsenic and phthalates). However, higher %mono-ethylhexyl phthalate (%MEHP), a putative indicator of phthalate diester metabolism, was associated with a larger change in tumor size (β = 0.015, 95% CI [0.003–0.03], P = 0.019).ConclusionWe found no statistically significant association between the urinary levels of arsenic, cotinine, and phthalates metabolites and the response to cervical cancer treatment as measured by the change in tumor size. Still, our results suggested that phthalates metabolism may be associated with response to treatment for locally advanced cervical cancer. However, these observations are preliminary and will require confirmation in a larger, more definitive investigation.

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“…Within 15 minutes of urine collection, samples were frozen at -20°C and then transferred to the Environmental Health Center (Cluj-Napoca, Romania), where they were stored until analysis for cotinine, arsenic, and five phthalate monoesters. Urinary creatinine was measured according to a previously described procedure, (Neamtiu, Dumitrascu & Roba, 2014), for which the intra-assay coefficient of variation (CV) was 6.5%.…”

Section: Urine Samples Collection and Analysismentioning

confidence: 99%

“…Urinary cotinine -The analytic method for determination of urinary cotinine was previously described in detail (Neamtiu, Dumitrascu & Roba, 2014). In brief, cotinine was extracted with dichloromethane, dissolved in toluene and analyzed using a QP 2010 Plus NCI gas chromatograph (Shimadzu, Japan) coupled to a mass spectrometer (GC-MS) operated in the selective ion monitoring mode.…”

Section: Urine Samples Collection and Analysismentioning

confidence: 99%

Peer Review #2 of "Impact of exposure to tobacco smoke, arsenic, and phthalates on locally advanced cervical cancer treatment—preliminary results (v0.1)"

2016

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Background. Cancer research is a national and international priority, with the efficiency and effectiveness of current anti-tumor therapies being one of the major challenges with which physicians are faced. Objective. To assess the impact of exposure to tobacco smoke, arsenic, and phthalates on cervical cancer treatment. Methods. We investigated 37 patients with locally advanced cervical carcinoma who underwent chemotherapy and radiotherapy. We determined cotinine and five phthalate metabolites in urine samples collected prior to cancer treatment, by gas chromatography coupled to mass spectrometry, and urinary total arsenic by atomic absorption spectrometry with hydride generation. We used linear regression to evaluate the effects of cotinine, arsenic, and phthalates on the change in tumor size after treatment, adjusted for confounding variables. Results. We detected no significant associations between urinary cotinine, arsenic, or phthalate monoesters on change in tumor size after treatment, adjusted for urine creatinine, age, baseline tumor size, and cotinine (for arsenic and phthalates). However, higher %mono-ethylhexyl phthalate (%MEHP), a putative indicator of phthalate diester metabolism, was associated with a larger change in tumor size (β=0.015, 95%CI=0.003-0.03, P=0.019). Conclusion. We found no statistically significant association between the urinary levels of arsenic, cotinine, and phthalates metabolites and the response to cervical cancer treatment as measured by the change in tumor size. Still,

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Chemical Analysis of Urinary Cotinine in Subjects Exposed to A Mixture of Contaminants

Cited by 4 publications

References 4 publications

Arsenic Methyltransferase and Apolipoprotein E Polymorphism in Pregnant Women Exposed to Inorganic Arsenic in Drinking Water in Western Romania

Arsenic Methyltransferase and Apolipoprotein E Polymorphism in Pregnant Women Exposed to Inorganic Arsenic in Drinking Water in Western Romania

Impact of exposure to tobacco smoke, arsenic, and phthalates on locally advanced cervical cancer treatment—preliminary results

Peer Review #2 of "Impact of exposure to tobacco smoke, arsenic, and phthalates on locally advanced cervical cancer treatment—preliminary results (v0.1)"

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