Chemical analysis of the surface of microorganisms by X-ray photoelectron spectroscopy

Amory, D.E.

doi:10.1016/0378-1097(88)90110-3

“…Incidentally, it is noted that more extensive physico‐chemical analyses of non‐medically important yeast cell surfaces has been carried out using brewing yeasts [88, 89]. Hydrophobicity of the yeast cell surface, determined by hydrophobic interaction chromatography was caused by the presence of nitrogen‐rich proteinaceous groups, while furthermore hydrophobic brewing yeast strains also had higher isoelectric points than hydrophilic ones, similar to the physico‐chemical relationships revealed for bacterial strains.…”

Section: Mechanisms Of Microbial Adhesive Interactionsmentioning

confidence: 82%

Adhesive interactions between medically important yeasts and bacteria

Millsap

¹

,

Mei

²

,

Bos

³

et al. 1998

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Yeasts are being increasingly identified as important organisms in human infections. Adhesive interactions between yeasts and bacteria may contribute to yeast retention at body sites. Methods for studying adhesive interactions between bacterial strains are well known, and range from simple macroscopic methods to flow chamber systems with complex image analysis capabilities. The adhesive interactions between bacteria and yeasts have been studied employing several of the methods originally developed for studying adhesive interactions between bacteria. However, in many of the methods employed the larger size of the yeasts as compared with bacteria results in strong sedimentation of the yeasts, often invalidating the method adapted. In addition, most methods are semi-quantitative and do not properly control mass transport. Consequently, adhesive interaction mechanisms between yeasts and bacteria identified hitherto, including lectin binding and protein-protein interactions, must be regarded with caution. Extensive physico-chemical characteristics of yeast cell surfaces are not available and a physico-chemical mechanism has not yet been put forth. A new method for quantifying adhesive interactions between yeasts and bacteria is proposed, based on the use of a parallel plate flow chamber, in which the influence of adhering bacteria upon the kinetics of yeast adhesion and aggregation of the adhering yeasts is quantitatively evaluated, under carefully controlled mass transport.

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“…The electric potential at microbial cell surfaces is generated by ionized basic and acidic groups. Amory et al (1988) and van Haecht et al (1982) concluded from X-ray photoelectron spectroscopy data, that the negative charge of microorganisms is mainly due to deprotonated phosphate groups that are partly neutralized by protonated amino groups.…”

Section: Microbial Surface Chargementioning

confidence: 99%

“…1988;van Haecht et al 1982), zeta potential and electrophoretic mobility at p H 4.0 (Amory et al 1988) or at a more physiological p H (Biichs et al 1988, Abbott et al 1983, van Loosdrecht et al 1987b, Champluvier et al 1988. The corresponding parameters of the bacteria investigated were determined from the p H zeta-potential curves.…”

Section: Microbial Surface Chargementioning

confidence: 99%

Influence of physicochemical bacterial surface properties on adsorption to inorganic porous supports

Krekeler¹,

Ziehr²,

Klein³

1991

Appl Microbiol Biotechnol

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The effect of the hydrophobicity and the electrostatic charge of bacterial cell surfaces on the initial phase of adsorption to inorganic porous supports with SiO2 or A1203 as the main components was investigated. The physicochemical surface properties of various Gram-positive and Gram-negative bacteria were characterized by water contact angle and zeta-potential measurements. The influence of microbial charge on adsorption was investigated by varying the ionic strength of the suspending liquid. The amount of Escherichia coli cells adsorbed to Siran and Bi 86 supports increased with increasing electrolyte concentration. The effect of cell surface hydrophobicity on the extent of adsorption was demonstrated at high ionic strength (0.15 M NaC1) where charge effects were reduced. The supports applied in this study promoted the adsorption of hydrophilic bacteria.

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“…Incidentally, it is noted that more extensive physico-chemical analyses of non-medically important yeast cell surfaces has been carried out using brewing yeasts [88,89]. Hydrophobicity of the yeast cell surface, determined by hydrophobic interaction chromatography was caused by the presence of nitrogen-rich proteinaceous groups, while furthermore hydrophobic brewing yeast strains also had higher isoelectric points than hydrophilic ones, similar to the physico-chemical relationships revealed for bacterial strains.…”

Section: Physico-chemical Features Of Adhesive Interactions Between Ymentioning

confidence: 83%

Adhesive interactions between medically important yeasts and bacteria

Millsap¹

1998

FEMS Microbiology Reviews

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Yeasts are being increasingly identified as important organisms in human infections. Adhesive interactions between yeasts and bacteria may contribute to yeast retention at body sites. Methods for studying adhesive interactions between bacterial strains are well known, and range from simple macroscopic methods to flow chamber systems with complex image analysis capabilities. The adhesive interactions between bacteria and yeasts have been studied employing several of the methods originally developed for studying adhesive interactions between bacteria. However, in many of the methods employed the larger size of the yeasts as compared with bacteria results in strong sedimentation of the yeasts, often invalidating the method adapted. In addition, most methods are semi-quantitative and do not properly control mass transport. Consequently, adhesive interaction mechanisms between yeasts and bacteria identified hitherto, including lectin binding and protein-protein interactions, must be regarded with caution. Extensive physico-chemical characteristics of yeast cell surfaces are not available and a physico-chemical mechanism has not yet been put forth. A new method for quantifying adhesive interactions between yeasts and bacteria is proposed, based on the use of a parallel plate flow chamber, in which the influence of adhering bacteria upon the kinetics of yeast adhesion and aggregation of the adhering yeasts is quantitatively evaluated, under carefully controlled mass transport.

show abstract

Chemical analysis of the surface of microorganisms by X-ray photoelectron spectroscopy

Cited by 7 publications

References 3 publications

Adhesive interactions between medically important yeasts and bacteria

Adhesive interactions between medically important yeasts and bacteria

Influence of physicochemical bacterial surface properties on adsorption to inorganic porous supports

Adhesive interactions between medically important yeasts and bacteria

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