Chemical analysis of the human brain by imaging mass spectrometry

Abstract

Analysis of the chemical makeup of the brain enables a deeper understanding of several neurological processes. Molecular imaging that deciphers the spatial distribution of neurochemicals with high specificity and sensitivity...

Recent developments and applications of ambient mass spectrometry imaging in pharmaceutical research: an overview

“…When the morphological indicators in traditional histopathology are ambiguous, the evaluation of tissue chemicals typically provides more tangible information on the underlying condition. 10 The MS-targeted methodology differs from conventional molecular visualization using chemical discoloration, immunostaining, fluorescence, and radiolabeling in that it does not require prior knowledge of the target species to be used. 10 Researchers can more closely investigate the chemical makeup of tissues with the aid of this MSI property in order to appreciate the operation of many biochemical mechanisms that are crucial to biological systems and identify any anomalies that might be related to them.…”

Metabolomics-based mass spectrometry methods to analyze the chemical content of 3D organoid models

“…Several recent reviews highlight the details of the measurement process and information that can be obtained from MSI analysis. [46][47][48][49] the spatial information can be useful, the depth of molecular coverage is less than that of separations-based MS approaches, which is why both approaches are often used on the same sample. [50][51][52] Researchers have applied MSI in areas ranging from pharmaceutical drug discovery to basic biomedical research.…”

Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) in disease diagnosis: an overview

“…The DESI probe can instantly capture molecular fingerprints from live organs, skin, tissues, biological fluids, and volatiles, which holds enormous promise for its performance in point-of-care diagnostics. 15…”

“…Using tandem MS, high mass exactness, and isotopic distribution, individual species that appeared in the mass spectra are identified. 15 Although DESI-MSI tissue diagnosis does not require ion characterization of the individual ions (metabolites or lipids), ion characterization can identify significant biomolecules and their relationship to disease biochemistry.…”

“…The DESI probe can instantly capture molecular ngerprints from live organs, skin, tissues, biological uids, and volatiles, which holds enormous promise for its performance in point-ofcare diagnostics. 15 The DESI-MSI (Fig. 2) procedure is usually carried out on a tissue section with a thickness of about 10-15 mm.…”