Chemical Analysis of Pollen by FT-Raman and FTIR Spectroscopies

Kenđel, Adriana; Zimmermann, Boris

doi:10.3389/fpls.2020.00352

Cited by 55 publications

(74 citation statements)

References 80 publications

(135 reference statements)

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“…To avoid any problems generally encountered with biological samples (especially the possible presence of different quantities of water in the sample) [ 32 ], to propose a reproducible analysis, and in good agreement with previous study with pollen [ 11 ], we focused our analysis on the spectrum region 2000–600 cm −1 . In this analyzed region, some statistically significant specific bands possibly used for N. nucifera and N. lotus pollen authentication are also proposed ( Figure 5 b).…”

Section: Resultsmentioning

confidence: 83%

“…FTIR can discriminate between species highlighting IR bands present in only one species (qualitative markers) or IR bands having a significant difference between species (quantitative markers). This technique also provided important information for the identification of pollen [ 11 , 13 ]. Figure 5 a shows the FTIR spectra of the N. nucifera and N. lotus pollen materials.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, these wave number could originate from the chemical bonds of polysaccharides (around 1200–900 cm −1 (C−O, C−C, C−O−C, and C−OH stretches and deformations)), proteins (around 1650–1700 (amide I), 1535 (amide II), and 1445 cm −1 (CH 2 deformation)), lipids (around 1745 (C=O stretch), 1460 (CH 2 deformation) and 1165 cm −1 (C−O−C stretching in esters)), and polyamines (around 1605, 1515, 1205, 1170, 855, 830 and 815 cm −1 ) [ 11 , 13 , 33 ]. Proteins, acyl-lipids, polysaccharides, sporopollenin conjugates composed mainly long chain fatty acids, and diverse phenylpropanoids including hydroxycinnamic acids were all reported in different concentrations on the surface of the cell walls of mature pollen of all higher plants [ 11 , 34 ]. Here, a majority of the N. nucifera -specific bands were located in the 1200–800 cm −1 region, while an important part of the N. lotus -specific bands was observed in the 1700–1500 cm −1 region of the FTIR spectra.…”

Section: Resultsmentioning

confidence: 99%

“…This could be related to the difference in exine, the outer wall of pollen grain, ornamentation observed between the N. nucifera and the N. lotus ( Figure 1 ). Exine is a sporopollenin-rich tissue [ 11 ]. FTIR ratio signals have been previously used; in particular, the protein-to-polysaccharide ratio signals have been proposed as taxon-specific [ 11 ].…”

Section: Resultsmentioning

confidence: 99%

“…As a result, FITR analysis is sometimes preferred. In particular this method has shown great potential for quick and economical chemical characterization, identification, and pollen classification [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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A Critical Cross-Species Comparison of Pollen from Nelumbo nucifera Gaertn. vs. Nymphaea lotus L. for Authentication of Thai Medicinal Herbal Tea

Tungmunnithum

Renouard

Drouet

et al. 2020

Plants

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“Bau Luang” or Nelumbo nucifera Gaertn. is an aquatic medicinal herb that has been used as a component of traditional medicines, medicinal products, and herbal tea for good health, particularly in Asia. The stamen of N. nucifera is an important part of this medicinal plant that is used in the form of dried and/or powdered stamens for herbal tea as well as the main ingredient of some traditional remedies. However, there is another aquatic herb called “Bau Sai” or Nymphaea lotus L. that is distributed in similar locations. Living plants of these two aquatic species may be classified according to their morphology, but the dried and powdered stamens of these two medicinal species are difficult to distinguish. The major reason of adulteration is the higher price of Bau Luang stamen. As a result, various methods of authentication, such as pollen micromorphology evaluation using scanning electron microscopy (SEM) analysis, bioinformatics analysis of two nuclear and plastic DNA markers, phytochemical stamen profiling, and Fourier transform infrared (FTIR) analysis of stamen plant material authentication from Bau Luang and Bau Sai, have been used in this present research in order to avoid some adulteration and/or misuse between the dried stamens of Bau Luang and Bau Sai. These results showed that the micro-morphology of pollen (size of pollen grain, number of apertures, and surface ornamentation) from the SEM analysis, some phytochemical compounds and the FTIR sporopollenin-to-protein ratio signal analysis are potential tools for authentication and identification of these two medicinal plants from their dried-stamen materials. This model of investigation may also be used to distinguish dried plant material from other problematic plant groups.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Critical Cross-Species Comparison of Pollen from Nelumbo nucifera Gaertn. vs. Nymphaea lotus L. for Authentication of Thai Medicinal Herbal Tea

Tungmunnithum

Renouard

Drouet

et al. 2020

Plants

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An integrative environmental pollen diversity assessment and its importance for the Sustainable Development Goals

Hornick

Richter

Bastl

et al. 2021

Plants People Planet

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Societal Impact Statement Pollen relates to many aspects of human and environmental health, which protection and improvement are endorsed by the United Nations Sustainable Development Goals. By highlighting these connections in the frame of current challenges in monitoring and research, we discuss the need of more integrative and multidisciplinary pollen research related to societal needs, improving health of humans and our ecosystems for a sustainable future. Summary Pollen is at once intimately part of the reproductive cycle of seed plants and simultaneously highly relevant for the environment (pollinators, vector for nutrients, or organisms), people (food safety and health), and climate (cloud condensation nuclei and climate reconstruction). We provide an interdisciplinary perspective on the many and connected roles of pollen to foster a better integration of the currently disparate fields of pollen research, which would benefit from the sharing of general knowledge, technical advancements, or data processing solutions. We propose a more interdisciplinary and holistic research approach that encompasses total environmental pollen diversity (ePD) (wind and animal and occasionally water distributed pollen) at multiple levels of diversity (genotypic, phenotypic, physiological, chemical, and functional) across space and time. This interdisciplinary approach holds the potential to contribute to pressing human issues, including addressing United Nations Sustainable Development Goals, fostering social and political awareness of these tiny yet important and fascinating particles.

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Phleum pratense-pollen adaptive variations and pollen microbiome investigation under different climatic regions and prospects of allergenicity

Muhammad,

Saadia,

Richard

et al. 2024

Int J Biometeorol

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Chemical Analysis of Pollen by FT-Raman and FTIR Spectroscopies

Cited by 55 publications

References 80 publications

A Critical Cross-Species Comparison of Pollen from Nelumbo nucifera Gaertn. vs. Nymphaea lotus L. for Authentication of Thai Medicinal Herbal Tea

A Critical Cross-Species Comparison of Pollen from Nelumbo nucifera Gaertn. vs. Nymphaea lotus L. for Authentication of Thai Medicinal Herbal Tea

An integrative environmental pollen diversity assessment and its importance for the Sustainable Development Goals

Phleum pratense-pollen adaptive variations and pollen microbiome investigation under different climatic regions and prospects of allergenicity

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