Chemical analyses at micro and nano scale at SISSI-Bio beamline at Elettra-Sincrotrone Trieste

Birarda, Giovanni; Bedolla, Diana E.; Piccirilli, Federica; Stani, Chiaramaria; Vondracek, Hendrik; Vaccari, Lisa

doi:10.1117/12.2607751

Cited by 16 publications

(13 citation statements)

References 58 publications

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“…The same samples C1, F1, and F2 deposited onto Au TEM grids were measured at the Synchrotron Infrared Source for Spectroscopy and Imaging (SISSI-Bio) beamline at Elettra Sincrotrone Trieste (Italy) 59 , before being exposed to soft X-rays at TwinMic beamline. If X-ray beamlines both provide a chemical characterization at elemental level, the analysis at SISSI-Bio returns Fourier-transform infrared spectromicroscopy (µFTIR) data that can give information at molecular level, identifying the chemical moieties present within the sample.…”

Section: Methodsmentioning

confidence: 99%

Unexpected silicon localization in calcium carbonate exoskeleton of cultured and fossil coccolithophores

Bordiga

Lupi

Langer

et al. 2023

Sci Rep

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Coccolithophores, marine calcifying phytoplankton, are important primary producers impacting the global carbon cycle at different timescales. Their biomineral structures, the calcite containing coccoliths, are among the most elaborate hard parts of any organism. Understanding the morphogenesis of coccoliths is not only relevant in the context of coccolithophore eco-physiology but will also inform biomineralization and crystal design research more generally. The recent discovery of a silicon (Si) requirement for crystal shaping in some coccolithophores has opened up a new avenue of biomineralization research. In order to develop a mechanistic understanding of the role of Si, the presence and localization of this chemical element in coccoliths needs to be known. Here, we document for the first time the uneven Si distribution in Helicosphaera carteri coccoliths through three synchrotron-based techniques employing X-ray Fluorescence and Infrared Spectromicroscopy. The enrichment of Si in specific areas of the coccoliths point to a targeted role of this element in the coccolith formation. Our findings mark a key step in biomineralization research because it opens the door for a detailed mechanistic understanding of the role Si plays in shaping coccolith crystals.

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Section: Methodsmentioning

confidence: 99%

Unexpected silicon localization in calcium carbonate exoskeleton of cultured and fossil coccolithophores

Bordiga

Lupi

Langer

et al. 2023

Sci Rep

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“…In situ Attenuated Total Reflectance Fourier-Transform InfraRed (ATR–FTIR) spectroscopy. Experiments were carried out at the SISSI-Bio beamline at Elettra-Sincrotrone Trieste [ 68 ]. Spectra were acquired by using a Bruker Vertex70v interferometer, coupled to a Wide range FIR MIR beamsplitter and DTGS detector with a Bruker Platinum monolithic diamond ATR setup.…”

Section: Methodsmentioning

confidence: 99%

Polyvinylidene Fluoride Aerogels with Tailorable Crystalline Phase Composition

Torres-Rodríguez

Bedolla

D’Amico

et al. 2022

Gels

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In this work, polyvinylidene fluoride (PVDF) aerogels with a tailorable phase composition were prepared by following the crystallization-induced gelation principle. A series of PVDF wet gels (5 to 12 wt.%) were prepared from either PVDF–DMF solutions or a mixture of DMF and ethanol as non-solvent. The effects of the non-solvent concentration on the crystalline composition of the PVDF aerogels were thoroughly investigated. It was found that the nucleating role of ethanol can be adjusted to produce low-density PVDF aerogels, whereas the changes in composition by the addition of small amounts of water to the solution promote the stabilization of the valuable β and γ phases. These phases of the aerogels were monitored by FTIR and Raman spectroscopies. Furthermore, the crystallization process was followed by in-time and in situ ATR–FTIR spectroscopy. The obtained aerogels displayed specific surface areas > 150 m2 g−1, with variable particle morphologies that are dependent on the non-solvent composition, as observed by using SEM and Synchrotron Radiation Computed micro-Tomography (SR-μCT).

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“…The microspectroscopy FT-IR measurements (µ-FT-IR) were performed at the Chemical and Life Sciences branch of the SISSI beamline at Elettra Sincrotrone, (Trieste, Italy), using published methods [52].…”

Section: Micro-ft-ir Spectroscopymentioning

confidence: 99%

“…To avoid an overestimation of the deterioration product presence, the pixels corresponding to the oxalate crystals in sample #1 were cut out by the Quasar software [52]. Then, the FPA false colour maps (Figure 8a) were obtained by integrating the areas of the bands attributed to cellulose, lignin, and the deterioration products as described above and highlighted by the rectangular selections in Figure 7b.…”

Section: X-ray Diffraction (Xdr)mentioning

confidence: 99%

Biodeterioration Assessment of a Unique Old Pharaonic Kingdom Wooden Statue Using Advanced Diagnostic Techniques

et al. 2022

Self Cite

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A recently discovered Egyptian wooden statue of King Djedefre was studied together with some surrounding burial soil samples for assessing the statue biodeterioration. The wooden morphological characterisation identified the hardwood Acacia nilotica as the wood type. X-ray diffraction, micro-FT-IR spectroscopy, and scanning electron microscopy with an X-ray spectrometer were used to evaluate the wood deterioration degree and the soil contribution in wood biodeterioration. Microbiological analyses (fluorescent in situ hybridisation and polymerase chain reaction) were also performed to detect the microbial attack on the statue. The prolonged interaction of the statue with the burial environment caused a strong wood decay due to biotic (fungi and bacteria) and abiotic factors (e.g., humidity fluctuations of the burial environment), which caused the severe cracking and collapsing of the wood structures. The analyses of the burial soil mineral composition were relevant for obtaining an overall picture of the statue deterioration. The results are useful for planning the right conservation procedures for this very particular and important wooden statue. Furthermore, analysis of the woody cell wall will help in the selection of appropriate consolidation and recovery treatments. Because the statue is a unique single piece of wood, and the morphological observations indicated that it is a bald woman in a sitting position, this statue will provide new and interesting knowledge of Egyptian culture.

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Chemical analyses at micro and nano scale at SISSI-Bio beamline at Elettra-Sincrotrone Trieste

Cited by 16 publications

References 58 publications

Unexpected silicon localization in calcium carbonate exoskeleton of cultured and fossil coccolithophores

Unexpected silicon localization in calcium carbonate exoskeleton of cultured and fossil coccolithophores

Polyvinylidene Fluoride Aerogels with Tailorable Crystalline Phase Composition

Biodeterioration Assessment of a Unique Old Pharaonic Kingdom Wooden Statue Using Advanced Diagnostic Techniques

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