Chemical agents that inhibit pollen development: effects of the phenyl-cinnoline carboxylates SC-1058 and SC-1271 on the ultrastructure of developing wheat anthers (Triticum aestivum L. var Yecora roj�)

Schulz, Patricia; Cross, John W.; Almeida, Eduardo

doi:10.1007/bf00227655

Cited by 10 publications

(15 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The exine and tryphine constituents are contributed by the sporophytic tapetal cells (Dickinson et al ., 2000; Hernández‐Pinzón et al ., 1999; Heslop‐Harrison, 1968; Wu et al ., 1997). In wheat, genetic ablation of the tapetum or chemical interference in sporopollenin assembly results in male sterility (De Block et al ., 1997; El‐Ghazaly and Jensen, 1990; Schulz et al ., 1993). In this study, we found that the tapetal expression of TAA1 coincided very well with the microspore wall ontogeny with respect to exine formation and the production of Ubisch bodies.…”

Section: Discussionmentioning

confidence: 99%

Male gametophyte development in bread wheat (Triticum aestivum L.): molecular, cellular, and biochemical analyses of a sporophytic contribution to pollen wall ontogeny

et al. 2002

View full text Add to dashboard Cite

SummaryBread wheat (hexaploid AABBDD genome; 16 billion basepairs) is a genetically complex, self-pollinating plant with bisexual¯owers that produce short-lived pollen. Very little is known about the molecular biology of its gametophyte development despite a longstanding interest in hybrid seeds. We present here a comprehensive characterization of three apparently homeologous genes (TAA1a, TAA1b and TAA1c) and demonstrate their anther-speci®c biochemical function. These eight-exon genes, found at only one copy per haploid complement in this large genome, express speci®cally within the sporophytic tapetum cells. The presence of TAA1 mRNA and protein was evident only at speci®c stages of pollen development as the microspore wall thickened during the progression of free microspores into vacuolated-microspores. This temporal regulation matched the assembly of wall-impregnated sporopollenin, a phenylpropanoid-lipid polymer containing very long chain fatty alcohols (VLCFAlc), described in the literature. Our results establish that sporophytic genes contribute to the production of fatty alcohols: Transgenic expression of TAA1 afforded production of long/VLCFAlc in tobacco seeds (18 : 1; 20 : 1; 22 : 1; 24 : 0; 26 : 0) and in Escherichia coli (14 : 0; 16 : 0; 18 : 1), suggesting biochemical versatility of TAA1 with respect to cellular milieu and substrate spectrum. Pollen walls additionally contain fatty alcohols in the form of wax esters and other lipids, and some of these lipids are known to play a role in the highly speci®c sexual interactions at the pollen±pistil interface. This study provides a handle to study these and to manipulate pollen traits, and, furthermore, to understand the molecular biology of fatty alcohol metabolism in general.

show abstract

Section: Discussionmentioning

confidence: 99%

Male gametophyte development in bread wheat (Triticum aestivum L.): molecular, cellular, and biochemical analyses of a sporophytic contribution to pollen wall ontogeny

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Anthers of the WT and OsCER1A plants at different stages were fixed in 3.7% formaldehyde/acetic acid (v/v) and resin-embedded semi-thin sections (epoxy resin PolyBed 812, Polysciences) were made and observed as described by Zhu et al (2011) . For transmission electron microscopy (TEM), anthers were fixed in sodium phosphate buffer (pH 7.2) containing 4% (w/v) paraformaldehyde and 2.5% (v/v) glutaraldehyde and sectioned as described by Schulz et al (1993) . The sections were observed using an electron microscope (TECNAI G2 12, FEI) at 100–120 kV.…”

Section: Methodsmentioning

confidence: 99%

OsCER1 Plays a Pivotal Role in Very-Long-Chain Alkane Biosynthesis and Affects Plastid Development and Programmed Cell Death of Tapetum in Rice (Oryza sativa L.)

Ni¹,

Zhou²,

Li³

et al. 2018

Front. Plant Sci.

View full text Add to dashboard Cite

Cuticle waxes, which are primarily comprised of very-long-chain (VLC) alkanes, play an important role in plant reproductive development. ECERIFERUM1 (CER1) is recognized as the core element for VLC alkane biosynthesis in Arabidopsis (Arabidopsis thaliana). However, genes involved in the VLC alkane biosynthesis in rice remain unclear, and the alkane-form pathway in rice has still to be further explored. Here, we show that OsCER1, a homology of CER1, functions in VLC alkanes biosynthesis, which also could regulate anther development and plastids differentiation in rice. OsCER1 was highly expressed in the tapetum (stage 10) and bicellular pollen cells (stage 11). The decreased content of VLC alkanes (C25 and C27) in the OsCER1 knocked down plants as well as the increased content of C27 alkanes in the OsCER1 overexpression plants indicates that OsCER1 participates in VLC alkane biosynthesis. Downregulation of OsCER1 in rice led to sterility, and fewer amyloplasts within the mature pollen grains. In addition, the downregulation of OsCER1 in rice caused delayed tapetal programmed cell death and abnormal development of plastids in the tapetal cells. Furthermore, significantly altered levels of expression of genes involved in the pollen development were exhibited in the OsCER1 knocked down plants. These results indicate that OsCER1 is critical for VLC alkanes biosynthesis, plastids differentiation, and pollen development. This work provides insights into the VLC alkanes biosynthesis in anther development in rice.

show abstract

“…They bypass the issues like identification and restoration of male sterile line associated with GMS (McRae, 1985) and is alternative method to generate male sterility in plants. They act as gametocide and target the male gamete by creating abnormalities, thus effecting development of pollen, its viability and its dehiscence (Cross and Ladyman, 1991) without affecting the female fertility.…”

Section: Chemical Hybridizing Agents (Chas)mentioning

confidence: 99%

Induction of Male Sterility: A Boon for Plant Breeding

Visarada¹

2021

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Chemical agents that inhibit pollen development: effects of the phenyl-cinnoline carboxylates SC-1058 and SC-1271 on the ultrastructure of developing wheat anthers (Triticum aestivum L. var Yecora roj�)

Cited by 10 publications

References 26 publications

Male gametophyte development in bread wheat (Triticum aestivum L.): molecular, cellular, and biochemical analyses of a sporophytic contribution to pollen wall ontogeny

Male gametophyte development in bread wheat (Triticum aestivum L.): molecular, cellular, and biochemical analyses of a sporophytic contribution to pollen wall ontogeny

OsCER1 Plays a Pivotal Role in Very-Long-Chain Alkane Biosynthesis and Affects Plastid Development and Programmed Cell Death of Tapetum in Rice (Oryza sativa L.)

Induction of Male Sterility: A Boon for Plant Breeding

Contact Info

Product

Resources

About