Chemical Activities of E. Coli Adsorbed on a Resin

Hattori, Tsutomu; Furusaka, Chôseki

doi:10.1093/oxfordjournals.jbchem.a127235

Cited by 61 publications

(11 citation statements)

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“…substances, such as glucose or fumarate, to a lesser extent than unadsorbed cells. 8 ) This means that more negatively charged cells are less active as to oxidative metabolism than less negatively charged ones.…”

mentioning

confidence: 99%

Changes in Surface Charge, Respiratory Rate and Stainability with Crystal Violet of RestingEscherichia coliB Cells Anaerobically Exposed to an Alternating Current

Shimada¹,

Shimahara²

1985

Agricultural and Biological Chemistry

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mentioning

confidence: 99%

Changes in Surface Charge, Respiratory Rate and Stainability with Crystal Violet of RestingEscherichia coliB Cells Anaerobically Exposed to an Alternating Current

Shimada¹,

Shimahara²

1985

Agricultural and Biological Chemistry

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“…The fact that the length of each period in the first phase was observed to be the shortest in the medium of pH 7.3, the value being about one unit higher than the optimum pH for growth of free cells, is interpreted similarly as pH shift in the previous experiments (7), that is, a cationic layer on the surface of the resin affects the growth of adsorbed cells also in the case of continuous system. From equation (3) for the state of equilibrium in the second phase, the growth rate of adsorbed cells in a continuous system can be calculated as follows :…”

Section: Vol 18mentioning

confidence: 66%

“…The fact is very surprising since the chemical activities of adsorbed cells are lower than those of free cells (7,8,9). If many bacteria which attach on several liquid-solid interface in a natural habitat or in a laboratory grow at such a rapid rate, their growth on these interfaces may be more significant than have ever been considered.…”

Section: Vol 18mentioning

confidence: 99%

Growth of Escherichia Coli on the Surface of an Anion-Exchange Resin in Continuous Flow System

Hattori

1972

J. Gen. Appl. Microbiol.

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Suspension of Escherichia coli adsorbed on an anion-exchange resin, Dowex 1, was incubated in continuously flowing growth medium at D=1, the value being above a critical dilution rate. At D=1, it is possible to follow the growth of adsorbed cells separately from that of free cells since cells freely suspended in the liquid phase of culture are rapidly washed out from the system, though the latter population might be growing under the condition.Cell concentration in the liquid phase of culture at first increased stepwise with regular periodicity (first phase) and secondly kept a steady level in proportion to the initial number of adsorbed cells (second phase). Periodic change of cell concentration in the first phase is explained by division and detachment of new-born cells from the resin surface according to age distribution of adsorbed cells. The length of each period depends on the pH of the medium and reflects the generation time of adsorbed cells. From the level of cell concentration in the second phase, growth rate with adsorbed cells is calculated and it is concluded that the growth rate with adsorbed cells is considerably larger than that with free cells.

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“…It was also shown that the curves of concentration of anionic substrate-oxidative activity with attached cells shifted to higher substrate concentrations compared to those with free cells (19,20). These results were interpreted by one of the authors by introducing a model of the cationic layer (19). The model postulated around the counter anions of Dowex 1 a cationic layer which included attached cells and made the microenvironment more acidic than the environment in bulk solution.…”

Section: Adsorptivementioning

confidence: 93%

“…In previous studies on oxidation of several substrates by several kinds of bacteria, it was observed that pH-oxidative activity curves with cells attached on Dowex 1 shifted to higher pHs than those with free cells (18)(19)(20). It was also shown that the curves of concentration of anionic substrate-oxidative activity with attached cells shifted to higher substrate concentrations compared to those with free cells (19,20). These results were interpreted by one of the authors by introducing a model of the cationic layer (19).…”

Section: Adsorptivementioning

confidence: 99%

Adsorptive phenomena involving bacterial cells and an anion exchange resin.

Hattori

1985

J. Gen. Appl. Microbiol.

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Adsorptive phenomena involving Escherichia coli and an anion exchange resin, Dowex 1 in chloride form, were studied in terms of three categories; attachment, loose immobilization and repulsion. Attachment was directly quantified, but the latter two were estimated by the ratio between cell concentrations in the inter-particle space in resin sediment (Cr) and in the supernatant (C1). Attachment was accompanied by proportional desorption of chloride ions. Attachment and loose immobilization with unmodified and modified cells were affected by pH, suggesting that the number and dissociation balance of anionic and cationic groups of bacterial surface are involved in both of them. Attachment of more cells became difficult with the increase in attached cell density. This was attributed to the repulsion between free and attached cells. When cell concentration in bulk solution was not so high, the repulsion varied with the amount of attached cells and pH. Under the conditions where the repulsion was great, it was visible as a thin transparent zone at the boundary between resin sediment and supernatant. A previously postulated cationic layer around the resin is discussed in terms of the repulsion. When cell concentration in bulk solution increased greatly, free cells again attached to the resin. This was attributed to decreased repulsion and a possible mechanism is discussed.

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Chemical Activities of E. Coli Adsorbed on a Resin

Cited by 61 publications

References 0 publications

Changes in Surface Charge, Respiratory Rate and Stainability with Crystal Violet of RestingEscherichia coliB Cells Anaerobically Exposed to an Alternating Current

Changes in Surface Charge, Respiratory Rate and Stainability with Crystal Violet of RestingEscherichia coliB Cells Anaerobically Exposed to an Alternating Current

Growth of Escherichia Coli on the Surface of an Anion-Exchange Resin in Continuous Flow System

Adsorptive phenomena involving bacterial cells and an anion exchange resin.

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