Chemical activation of Piezo1 to enhance TRAIL-mediated apoptosis in glioblastoma cells

Abstract

Treatment with TRAIL + Yoda1• Cells were treated with 25 ng/mL TRAIL and 10μM Yoda1• U87 cells were treated for 24 h and LN18 for 4 h Flow cytometry assays• Annexin V and Propidium Iodide (AV-PI) assays were used to measure cell viability, apoptosis, and necrosis• Cells were treated with DMSO (10μM), Yoda1 (10μM), TRAIL (25 ng/mL), low dose TMZ (50μM) and high dose TMZ (200 μM)