Chemical activation of oocytes in different types of sperm abnormalities in cases of low or failed fertilization after ICSI: a prospective pilot study

Moaz, Mohamed; Khattab, S.; Foutouh, Ismail Aboul; Mohsen, Eman Abdel

doi:10.1016/s1472-6483(10)61025-5

Cited by 42 publications

(19 citation statements)

References 15 publications

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“…One of the earliest studies was a case report on ICSI couples characterized by poor fertilization, where the use of calcium ionophore post-injection lead to reasonable zygote formation [162]. Another cohort of 56 patients with a history of inconsistent fertilization due to severe sperm head abnormalities used ionomycin to enhance fertilization but failed to generate good quality embryos [163]. In another study where globozoospermic ( n = 10) and oligo-astheno-terato-zoospermic ( n = 20) men were treated by injecting CaCl 2 concurrently with ICSI followed by sequential exposure of calcium ionophore not only gained superior fertilization but provided clinical pregnancies and deliveries of healthy neonates [155].…”

Section: Treatment Optionsmentioning

confidence: 99%

Understanding fertilization through intracytoplasmic sperm injection (ICSI)

Neri¹,

Lee²,

Rosenwaks³

et al. 2014

Cell Calcium

123

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Summary Since the establishment of in vitro fertilization, it became evident that almost half of the couples failed to achieve fertilization and this phenomenon was attributed to a male gamete dysfunction. The adoption of assisted fertilization techniques particularly ICSI has been able to alleviate male factor infertility by granting the consistent ability of a viable spermatozoon to activate an oocyte. Single sperm injection, by pinpointing the beginning of fertilization, has been an invaluable tool in clarifying the different aspects of early fertilization and syngamy. However, even with ICSI some couples fail to fertilize due to ooplasmic dysmaturity in relation to the achieved nuclear maturation marked by the extrusion of the first polar body. More uncommon are cases where the spermatozoa partially or completely lack the specific oocyte activating factor. In this work, we review the most relevant aspects of fertilization and its failure through assisted reproductive technologies. Attempts at diagnosing and treating clinical fertilization failure are described.

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Section: Treatment Optionsmentioning

confidence: 99%

Understanding fertilization through intracytoplasmic sperm injection (ICSI)

Neri¹,

Lee²,

Rosenwaks³

et al. 2014

Cell Calcium

123

View full text Add to dashboard Cite

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“…However, there was no significant effect on early cleavage or number of good embryos in all subgroups of sperm morphological abnormalities [7] suggesting that in several cases another factor besides activation of oocytes is responsible for the success of the ICSI procedure. In pigs, the failure of male pronucleus formation was the major cause for the failure of fertilization in activated ICSI oocytes [8].…”

Section: Introductionmentioning

confidence: 80%

Sperm chromatin stability and its relationship with fertilization rate after Intracytoplasmic Sperm Injection (ICSI) in an assisted reproduction program

García¹,

Noriega-Hoces²,

Gonzales

2007

J Assist Reprod Genet

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Objective Evaluate sperm chromatin stability and its relationship with the rate of fertilization after procedures of intracytoplasmic sperm injection (ICSI) in a program of assisted reproduction. Design Prospective study. Setting Institute of Gynecology and Reproduction. Patients Thirty-three women with their respective partners (12 couples in the study group and 21 couples in the control group) participating in a program of assisted reproduction. The study group was defined as men with >30% of nondecondensed spermatozoa (high sperm chromatin stability). Interventions A part of each seminal sample was used to evaluate sperm chromatin stability under SDS and EDTA treatment and the second aliquot was used for the ICSI procedure. Fertilization was evaluated 16-18 h post sperm injection at a pronuclear stage. The fertilized oocytes were further cultured for 24-48 h before transfer to the patient. Main outcome measures Fertilization rate.Results Thirty-five oocytes (35.7%) in the group of study and 109 oocytes (78.9%) in the control group showed two pronuclei (P<0.001). The coefficient of determination between the SDS+EDTA (Grade 2) and rate of fertilization was r 2 =0.85 (P<0.001) and the coefficient of regression was 1.72±0.19 (β±ES) (P<0.001). Conclusions High sperm chromatin stability is a factor which reduces the rate of fertilization after ICSI procedure.

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“…For the human oocyte, electrical stimulation (electroporation) [10], mechanical activation [11], treatment with calcium ionophore (A231287 [12], ionomycin [13]), treatment with strontium chloride (SrCl 2 ) [14], PLCζ [15], puromycin [16] and roscovitine [17] have been reported as the methods of AOA. Puromycin and roscovitine are protein synthesis inhibitors and activate oocytes by inhibiting synthesis of MPF without causing Ca 2+ increase.…”

Section: Methods Of Oocyte Activationmentioning

confidence: 99%