Chemical activation of nitrocellulose membranes for peptide antigen‐antibody binding studies: Direct substitution of the nitrate group with diaminoalkane

Másson, Már; Lauritzen, Edgar; Holm, Arne

doi:10.1002/elps.11501401137

Cited by 20 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Preparation of Capturing Antibody Pad. The capturing antibody pad was prepared by the covalent binding of anti-IgG to a glutaraldehyde-activated nitrocellulose membrane following the protocol of Masson . The nitrocellulose membrane (4 cm × 4 cm) was first treated with 2.5% diaminoheptane solution for 1 h with constant agitation.…”

Section: Methodsmentioning

confidence: 99%

Disposable Electrochemical Immunosensor Diagnosis Device Based on Nanoparticle Probe and Immunochromatographic Strip

et al. 2007

View full text Add to dashboard Cite

We describe a disposable electrochemical immunosensor diagnosis device that integrates the immunochromatographic strip technique with an electrochemical immunoassay and exploits quantum dot (QD, CdS@ZnS) as labels for amplifying signal output. The device takes advantage of the speed and low cost of the conventional immunochromatographic strip test and the high sensitivity of the nanoparticle-based electrochemical immunoassay. A sandwich immunoreaction was performed on the immunochromatographic strip, and the captured QD labels in the test zone were determined by highly sensitive stripping voltammetric measurement of the dissolved metallic component (cadmium) with a disposable screen-printed electrode, which is embedded underneath the membrane on the test zone. The new device coupled with a portable electrochemical analyzer shows great promise for in-field and point-of-care quantitative testing of disease-related protein biomarkers. The parameters (e.g., voltammetric measurement of QD labels, antibody immobilization, the loading amount of QD-antibody, and the immunoreaction time) that govern the sensitivity and reproducibility of the device were optimized with IgG model analyte. The voltammetric response of the optimized device is highly linear over the range of 0.1-10 ng mL(-1) IgG, and the limit of detection is estimated to be 30 pg mL(-1) in association with a 7-min immunoreaction time. The detection limit was improved to 10 pg mL(-1) using a 20-min immunoreaction time. The device has been successfully applied for the detection of prostate-specific antigen (PSA) in human serum sample with a detection limit of 20 pg mL(-1). The results were validated by using the commercial PSA enzyme-linked immunosorbent assay kit and showed high consistency. The new disposable electrochemical diagnosis device thus provides a rapid, clinically accurate, and quantitative tool for protein biomarker detection.

show abstract

Section: Methodsmentioning

confidence: 99%

Disposable Electrochemical Immunosensor Diagnosis Device Based on Nanoparticle Probe and Immunochromatographic Strip

et al. 2007

View full text Add to dashboard Cite

show abstract

“…NC is unique, when compared to other microporous membranes, in its ability to distinguish between single-and double-stranded nucleic acids, small and large proteins, short and long nucleic acids, and complexed versus uncomplexed molecules [ 22 ].…”

Section: Immobilization Mechanismmentioning

confidence: 99%

Western Blotting: An Introduction

Kurien

Scofield

2015

Methods in Molecular Biology

138

View full text Add to dashboard Cite

Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. Protein blotting has evolved greatly since the inception of this protocol, allowing protein transfer to be accomplished in a variety of ways.

show abstract

“…31 Nitrocellulose disks ͑2.5 cm diameter͒ were immersed in 1,6 diaminohexane ͑2.5% w/v, pH 11.9͒ for 60 min under constant rotary agitation. The membranes were next washed under constant agitation for approximately 12 h in 1-M acetic acid followed by 12 h in ultrapure water with several changes of each medium.…”

Section: Preparation Of the Nitrocellulose Membranementioning

confidence: 99%

In vitro characterization of a novel, tissue-targeted ultrasonic contrast system with acoustic microscopy

Lanza

Trousil

Wallace

et al. 1998

The Journal of the Acoustical Society of America

102

View full text Add to dashboard Cite

Targeted ultrasonic contrast systems are designed to enhance the reflectivity of selected tissues in vivo [Lanza et al., Circulation 94, 3334 (1996)]. In particular, these agents hold promise for the minimally invasive diagnosis and treatment of a wide array of pathologies, most notably tumors, thromboses, and inflamed tissues. In the present study, acoustic microscopy was used to assess the efficacy of a novel, perfluorocarbon based contrast agent to enhance the inherent acoustic reflectivity of biological and synthetic substrates. Data from these experiments were used to postulate a simple model describing the observed enhancements. Frequency averaged reflectivity (30-55 MHz) was shown to increase 7.0 +/- 1.1 dB for nitrocellulose membranes with targeted contrast. Enhancements of 36.0 +/- 2.3 dB and 8.5 +/- 0.9 dB for plasma and whole blood clots, respectively, were measured between 20 and 35 MHz. A proposed acoustic transmission line model predicted the targeted contrast system would increase the acoustic reflectivity of the nitrocellulose membrane, whole blood clot, and fibrin plasma clot by 2.6, 8.0, and 31.8 dB, respectively. These predictions were in reasonable agreement with the experimental results of this paper. In conclusion, acoustic microscopy provides a rapid and sensitive approach for in vitro chracterization, development, and testing of mathematical models of targeted contrast systems. Given the current demand for targeted contrast systems for medical diagnostic and therapeutic use, the use of acoustic microscopy may provide a useful tool in the development of these agents.

show abstract

Chemical activation of nitrocellulose membranes for peptide antigen‐antibody binding studies: Direct substitution of the nitrate group with diaminoalkane

Cited by 20 publications

References 19 publications

Disposable Electrochemical Immunosensor Diagnosis Device Based on Nanoparticle Probe and Immunochromatographic Strip

Disposable Electrochemical Immunosensor Diagnosis Device Based on Nanoparticle Probe and Immunochromatographic Strip

Western Blotting: An Introduction

In vitro characterization of a novel, tissue-targeted ultrasonic contrast system with acoustic microscopy

Contact Info

Product

Resources

About