Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells

Suzuki, Yutaka; Haga, Satoshi; Katoh, Daiki; So, KF; Choi, Ki-Seok; Jung, Usuk; Lee, Hong-Gu; Katoh, K.; Roh, Sanggun

doi:10.1016/j.bbrc.2015.08.105

Cited by 15 publications

(35 citation statements)

References 35 publications

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“…Studies with immortalized bovine MECs treated with chemerin revealed upregulated expression of genes associated with fatty acid synthesis, glucose uptake, and casein synthesis; thus, it is postulated that chemerin may play a role of lactogenesis regulator in bovine mammary epithelium. Surprisingly, adiponectin reduced the expression of CMKLR1 receptor, without altering CCRL2 expression [30]. These results imply that adiponectin is not only able to counteract the effects of leptin but also able to regulate the influence of chemerin on mammary epithelial cells.…”

Section: Adipokinesmentioning

confidence: 80%

“…Two types of receptors, adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2), have distinct distribution patterns in different tissues. Both receptors were shown to be expressed in normal mammary epithelial cells [29,30]. Binding of adiponectin to its receptor activates adenosine monophosphate-activated protein kinase (AMPK), a nutrient-sensing enzyme, which regulates several key pathways involved in protein synthesis and cellular energy metabolism.…”

Section: Adipokinesmentioning

confidence: 99%

“…Binding of adiponectin to its receptor activates adenosine monophosphate-activated protein kinase (AMPK), a nutrient-sensing enzyme, which regulates several key pathways involved in protein synthesis and cellular energy metabolism. One of the few researches on bovine mammary gland disclosed that adiponectin expression in the mammary gland decreases in the peak and late-lactation period, although adiponectin receptor 1 (AdipoR1) expression increases in the same period [30]. Moreover, leptin/adiponectin ratio is directly proportional to the size of stem cell population in vivo.…”

Section: Adipokinesmentioning

confidence: 99%

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Stromal-Epithelial Interactions during Mammary Gland Development

Dzięgelewska¹,

Gajewska²

2019

Stromal Cells - Structure, Function, and Therapeutic Implications

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Mammary gland is an organ, which undergoes the majority of its development in the postnatal life of mammals. The complex structure of the mammary gland comprises epithelial and myoepithelial cells forming the parenchymal tissue and adipocytes, fibroblasts, vascular endothelial cells, and infiltrating immune cell composing the stromal compartment. During puberty and in adulthood, circulating hormones released from the pituitary and ovaries regulate the rate of development and functional differentiation of the mammary epithelium. In addition, growing body of evidence shows that interactions between the stromal and parenchymal compartments of the mammary gland play a crucial role in mammogenesis. This regulation takes place on a paracrine level, by locally synthesized growth factors, adipokines, and cytokines, as well as via direct cell-cell interactions. This chapter summarizes the current knowledge about the complex nature of interactions between the mammary epithelium and stroma during mammary gland development in different mammalian species.

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Section: Adipokinesmentioning

confidence: 80%

Section: Adipokinesmentioning

confidence: 99%

Section: Adipokinesmentioning

confidence: 99%

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Stromal-Epithelial Interactions during Mammary Gland Development

Dzięgelewska¹,

Gajewska²

2019

Stromal Cells - Structure, Function, and Therapeutic Implications

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“…In this study, we investigated how MEN1 /Menin regulated the synthesis of milk protein in mammary epithelial cells. The bovine epithelial cell line MAC-T was used as a model for studying milk protein synthesis regulation 14–16 . The activation of the PI3K/Akt/mTOR pathway 12, 13, 24 was revealed to be essential for the initiation of the transcription and translation of milk protein genes.…”

Section: Discussionmentioning

confidence: 99%

“…Motivated by the search for the role of MEN1 as a regulator in the milk protein synthesis that mediated by the mTOR signaling pathway in mammary glands, this study investigated the direct effects of MEN1 in both the bovine mammary epithelial cell line MAC-T 14–16 and bovine mammary gland tissues.…”

Section: Introductionmentioning

confidence: 99%

MEN1/Menin regulates milk protein synthesis through mTOR signaling in mammary epithelial cells

Wang

et al. 2017

Sci Rep

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The MEN1 gene, which encodes the protein Menin, was investigated for its regulatory role in milk protein synthesis in mammary glands. Menin responds to nutrient and hormone levels via the PI3K/Akt/mTOR pathway. Bovine mammary epithelial cells and tissues were used as experimental models in this study. The results revealed that the milk protein synthesis capacity of mammary epithelial cells could be regulated by MEN1/Menin. The overexpression of Menin caused significant suppression of factors involved in the mTOR pathway, as well as milk protein κ-casein (CSNK). In contrast, a significant increase in these factors and CSNK was observed upon MEN1/Menin knockdown. The repression of MEN1/Menin on the mTOR pathway was also observed in mammary gland tissues. Additionally, MEN1/Menin was found to elicit a negative response on prolactin (PRL) and/or insulin (INS), which caused a similar downstream impact on mTOR pathway factors and milk proteins. Collectively, our data indicate that MEN1/Menin could play a regulatory role in milk protein synthesis through mTOR signaling in the mammary gland by mediating the effects of hormones and nutrient status. The discovery of Menin’s role in mammary glands suggests Menin could be potential new target for the improvement of milk performance and adjustment of lactation period of dairy cows.

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Menin Modulates Mammary Epithelial Cell Numbers in Bovine Mammary Glands Through Cyclin D1

Shi

et al. 2017

J Mammary Gland Biol Neoplasia

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Menin, the protein encoded by the MEN1 gene, is abundantly expressed in the epithelial cells of mammary glands. Here, we found MEN1/menin expression slowly decreased with advancing lactation but increased by the end of lactation. It happened that the number of bovine mammary epithelial cells decreases since lactation, suggesting a role of menin in the control of mammary epithelial cell growth. Indeed, reduction of menin expression through MEN1-specific siRNA transfection in the bovine mammary epithelial cells caused cell growth arrest in G1/S phase. Decreased mRNA and protein expression of Cyclin D1 was observed upon MEN1 knockdown. Furthermore, menin was confirmed to physically bind to the promoter region of Cyclin D1 through a ChIP assay, indicating that menin plays a regulatory role in mammary epithelial cell cycle progression. Moreover, lower expression of MEN1/menin induced increased epithelial cell apoptosis and caused extracellular matrix remodeling by down-regulating its associated genes, such as DSG2 and KRT5, suggesting that menin’s role may also be involved in the control of cell–cell adhesion in normal mammary glands. Taken together, our data revealed an unknown molecular function of menin in epithelial cell proliferation, which may be important in the regulation of lactation behavior of mammary glands.Electronic supplementary materialThe online version of this article (10.1007/s10911-017-9385-8) contains supplementary material, which is available to authorized users.

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Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells

Cited by 15 publications

References 35 publications

Stromal-Epithelial Interactions during Mammary Gland Development

Stromal-Epithelial Interactions during Mammary Gland Development

MEN1/Menin regulates milk protein synthesis through mTOR signaling in mammary epithelial cells

Menin Modulates Mammary Epithelial Cell Numbers in Bovine Mammary Glands Through Cyclin D1

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