Chemerin Impact on Alternative mRNA Transcription in the Porcine Luteal Cells

Abstract

Chemerin participates in the regulation of processes related to physiological and disorder mechanisms in mammals, including metabolism, obesity, inflammation, and reproduction. In this study, we have investigated chemerin influence on alternative mRNA transcription within the porcine luteal cell transcriptome, such as differential expression of long non-coding RNAs (DELs) and their interactions with differentially expressed genes (DEGs), differences in alternative splicing of transcripts (DASs), and allele-specific expression (ASEs) related to the single nucleotide variants (SNVs) frequency. Luteal cells were collected from gilts during the mid-luteal phase of the oestrous cycle. After in vitro culture of cells un-/treated with chemerin, the total RNA was isolated and sequenced using the high-throughput method. The in silico analyses revealed 24 DELs cis interacting with 6 DEGs and trans-correlated with 300 DEGs, 137 DASs events, and 18 ASEs. The results enabled us to analyse metabolic and signalling pathways in detail, providing new insights into the effects of chemerin on the corpus luteum functions related to inflammatory response, leukocyte infiltration, the occurrence of luteotropic and luteolytic signals (leading to apoptosis and/or necroptosis). Validation of the results using qPCR confirmed the predicted expression changes. Chemerin at physiological concentrations significantly modifies the transcription processes in the porcine luteal cells.

Adaptation of the Porcine Pituitary Transcriptome, Spliceosome and Editome during Early Pregnancy

“…The mRNAs levels and transcription-regulatory mechanisms, such as lncRNAs production, alternative splicing of transcripts and promoting the production of transcripts encoded by one of the alleles available in the genetic material, were analyzed to investigate the effect of the early gestation period milieu on porcine AP cells. The in silico analyses were mainly performed according to Paukszto et al [ 162 ] and Makowczenko et al [ 163 ].…”

“…Identification of lncRNAs in the porcine AP cells was performed according to Shen et al’s [ 161 ] multi-stage procedure with modifications [ 162 , 163 ]. Initially, at the filtering stage, t low expression, protein-coding, single-exon and too short transcripts (length < 200 nt) were excluded from the processed dataset.…”

Chemerin: A Functional Adipokine in Reproductive Health and Diseases

“…In porcine luteal cells, r-chemerin treatment altered the expression of biomarkers related to angiogenesis and apoptosis [ 67 ]. The regulatory effects of chemerin on corpus luteum in pigs were further verified by transcriptomic analysis, which showed that chemerin takes part in the processes including steroids and prostaglandins synthesis, inflammatory response as well as luteotropic and luteolytic signals [ 68 , 69 ].…”

Apoptosis, autophagic cell death, and necroptosis: different types of programmed cell death in bovine corpus luteum regression

“…Necroptosis also induces luteolysis in humans [25,26], pigs [27], and cattle [28]. In this article, we reviewed recent findings regarding the mechanisms of these three types of PCD and their contributions to luteolysis in mammals, including cattle.…”

“…In addition, ACD contributes to luteolysis in humans [ 17 , 18 ], rats [ 19 , 20 ], goats [ 21 ], pigs [ 22 ], and cattle [ 23 , 24 ]. Necroptosis also induces luteolysis in humans [ 25 , 26 ], pigs [ 27 ], and cattle [ 28 ]. In this article, we reviewed recent findings regarding the mechanisms of these three types of PCD and their contributions to luteolysis in mammals, including cattle.…”