Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis

Bors, Kinga; Dobrzyń, Kamil; Kieżun, Marta; Malinowska, Agata; Świderska, Bianka; Kamiński, Tadeusz; Smolińska, Nina

doi:10.3390/cells11071161

Cited by 5 publications

(6 citation statements)

References 88 publications

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“…We hypothesized that the energy supply was necessary after the initiation of implantation. In addition to the change in the endometrial signaling pathway, there was also an influence of the number of conceptuses [ 37 ]. Variations within the number of conceptuses result in variable levels of their signals in the uterine lumen; therefore, the maternal response could be different, which is also related to the number of corpora lutea.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs

Wang¹,

Xue

Liu

et al. 2023

BMC Genomics

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Background Endometrial receptivity plays a vital role in the success of embryo implantation. However, the temporal proteomic profile of porcine endometrium during embryo implantation is still unclear. Results In this study, the abundance of proteins in endometrium on days 9, 10, 11, 12, 13, 14, 15 and 18 of pregnancy (D9, 10, 11, 12, 13, 14, 15 and 18) was profiled via iTRAQ technology. The results showed that 25, 55, 103, 91, 100, 120, 149 proteins were up-regulated, and 24, 70, 169, 159, 164, 161, 198 proteins were down-regulated in porcine endometrium on D10, 11, 12, 13, 14, 15 and 18 compared with that on D9, respectively. Among these differentially abundance proteins (DAPs), Multiple Reaction Monitoring (MRM) results indicated that S100A9, S100A12, HRG and IFI6 were differentially abundance in endometrial during embryo implantation period. Bioinformatics analysis showed that the proteins differentially expressed in the 7 comparisons were involved in important processes and pathways related to immunization, endometrial remodeling, which have a vital effect on embryonic implantation. Conclusion Our results reveal that retinol binding protein 4 (RBP4) could regulate the cell proliferation, migration and apoptosis of endometrial epithelial cells and endometrial stromal cells to affect embryo implantation. This research also provides resources for studies of proteins in endometrium during early pregnancy.

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Section: Discussionmentioning

confidence: 99%

“…Variations within the number of conceptuses result in variable levels of their signals in the uterine lumen; therefore, the maternal response could be different, which is also related to the number of corpora lutea. Variation in the number of corpora lutea may result in different levels of P4 affecting the endometrium [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs

Wang¹,

Xue

Liu

et al. 2023

BMC Genomics

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“…Proteomic analysis was performed at the Mass Spectrometry Laboratory at the Institute of Biochemistry and Biophysics of the Polish Academy of Sciences. Samples were digested using the filter-aided sample preparation (FASP) method 31 according to the previously described protocol 32 , with minor modifications. In brief, 150 µg of total proteins was incubated for an hour with 20 mM tris(2-carboxyethyl)phosphine (TCEP) at 60 °C to reduce cysteine residues.…”

Section: Methodsmentioning

confidence: 99%

Visfatin impact on the proteome of porcine luteal cells during implantation

Kopij,

Kiezun,

Gudelska

et al. 2024

Sci Rep

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Visfatin (VIS) is a hormone belonging to the adipokines’ group secreted mainly by the adipose tissue. VIS plays a crucial role in the control of energy homeostasis, inflammation, cell differentiation, and angiogenesis. VIS expression was confirmed in the hypothalamic–pituitary–gonadal (HPG) axis structures, as well as in the uterus, placenta, and conceptuses. We hypothesised that VIS may affect the abundance of proteins involved in the regulation of key processes occurring in the corpus luteum (CL) during the implantation process in pigs. In the present study, we performed the high-throughput proteomic analysis (liquid chromatography with tandem mass spectrometry, LC–MS/MS) to examine the in vitro influence of VIS (100 ng/mL) on differentially regulated proteins (DRPs) in the porcine luteal cells (LCs) on days 15–16 of pregnancy (implantation period). We have identified 511 DRPs, 276 of them were up-regulated, and 235 down-regulated in the presence of VIS. Revealed DRPs were assigned to 162 gene ontology terms. Western blot analysis of five chosen DRPs, ADAM metallopeptidase with thrombospondin type 1 motif 1 (ADAMTS1), lanosterol 14-α demethylase (CYP51A1), inhibin subunit beta A (INHBA), notch receptor 3 (NOTCH3), and prostaglandin E synthase 2 (mPGES2) confirmed the veracity and accuracy of LC–MS/MS method. We indicated that VIS modulates the expression of proteins connected with the regulation of lipogenesis and cholesterologenesis, and, in consequence, may be involved in the synthesis of steroid hormones, as well as prostaglandins’ metabolism. Moreover, we revealed that VIS affects the abundance of protein associated with ovarian cell proliferation, differentiation, and apoptosis, as well as CL new vessel formation and tissue remodelling. Our results suggest important roles for VIS in the regulation of ovarian functions during the peri-implantation period.

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“…TMT-labeled peptides were fractionated using the high-pH reversed-phase method into 25 fractions. The system and gradient parameters are described in our previous publication [28].…”

Section: Reversed-phase Peptide Fractionation Of Tmt-labeled Peptidesmentioning

confidence: 99%

Proteome Profiling of the Dystrophic mdx Mice Diaphragm

Mucha,

Myszka,

Podkalicka

et al. 2023

Biomolecules

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Mdx mice with a spontaneous mutation in exon 23 of the Dmd gene represent the most common model to investigate the pathophysiology of Duchenne muscular dystrophy (DMD). The disease, caused by the lack of functional dystrophin, is characterized by irreversible impairment of muscle functions, with the diaphragm affected earlier and more severely than other skeletal muscles. We applied a label-free (LF) method and the more thorough tandem mass tag (TMT)-based method to analyze differentially expressed proteins in the diaphragm of 6-week-old mdx mice. The comparison of both methods revealed 88 commonly changed proteins. A more in-depth analysis of the TMT-based method showed 953 significantly changed proteins, with 867 increased and 86 decreased in dystrophic animals (q-value < 0.05, fold-change threshold: 1.5). Consequently, several dysregulated processes were demonstrated, including the immune response, fibrosis, translation, and programmed cell death. Interestingly, in the dystrophic diaphragm, we found a significant decrease in the expression of enzymes generating hydrogen sulfide (H2S), suggesting that alterations in the metabolism of this gaseous mediator could modulate DMD progression, which could be a potential target for pharmacological intervention.

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Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis

Cited by 5 publications

References 88 publications

Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs

Proteomic profiles and the function of RBP4 in endometrium during embryo implantation phases in pigs

Visfatin impact on the proteome of porcine luteal cells during implantation

Proteome Profiling of the Dystrophic mdx Mice Diaphragm

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