Chelerythrine Rapidly Induces Apoptosis through Generation of Reactive Oxygen Species in Cardiac Myocytes

Yamamoto, Shimako; Seta, Koichi; Morisco, Carmine; Vatner, Stephen F.; Sadoshima, Junichi

doi:10.1006/jmcc.2001.1446

Cited by 111 publications

(107 citation statements)

References 52 publications

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“…In all assays, confluent cultures were pretreated with 10 M chelerythrine (EMD Chemicals, Gibbstown, NJ, USA) for 30 minutes to induce apoptosis [43,44]. Cells were then incubated with rhAmelogenin or Fraction C. Cell viability was determined using MTT assay after 24 hours of treatment.…”

Section: Apoptosis Assaysmentioning

confidence: 99%

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

et al. 2013

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Enamel matrix derivative (EMD), a decellularized porcine extracellular matrix (ECM), is used clinically in periodontal tissue regeneration. Amelogenin, EMD's principal component, spontaneously assembles into nanospheres in vivo, forming an ECM complex that releases proteolytically cleaved peptides. However, the role of amelogenin or amelogenin peptides in mediating osteoblast response to EMD is not clear. Human MG63 osteoblast-like cells or normal human osteoblasts were treated with recombinant human amelogenin or a 5 kDa tyrosine-rich amelogenin peptide (TRAP) isolated from EMD and the effect on osteogenesis, local factor production, and apoptosis assessed. Treated MG63 cells increased alkaline phosphatase specific activity and levels of osteocalcin, osteoprotegerin, prostaglandin E 2 , and active/latent TGF-1, an effect sensitive to the effector and concentration. Primary osteoblasts exhibited similar, but less robust, effects. TRAP-rich 5 kDa peptides yielded more mineralization than rhAmelogenin in osteoblasts in vitro. Both amelogenin and 5 kDa peptides protected MG63s from chelerythrine-induced apoptosis. The data suggest that the 5 kDa TRAP-rich sequence is an active amelogenin peptide that regulates osteoblast differentiation and local factor production and prevents osteoblast apoptosis.

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Section: Apoptosis Assaysmentioning

confidence: 99%

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

et al. 2013

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“…43 ), human uveal melanoma (OCM-1) (ref. 39 ), human neuroblastoma (SH-SY5Y), and colon carcinoma (HCT116) 43 cell lines and neonatal rat cardiac myocytes 44 .…”

Section: Effects On Programmed Cell Deathmentioning

confidence: 99%

“…Yamamoto et al investigated that CHE, inhibitor of PKC, rapidly and potently induced cell death in neonatal rat cardiac myocytes 44 . It was found that generation of ROS rather than inhibition of PKC critically mediated CHE-induced rapid myocyte apoptosis.…”

Section: Che-/sa-mediated Apoptosis Induced By Generation Of Reactivementioning

confidence: 99%

“…These results suggest that CHE rapidly induced cardiac myocyte apoptosis and that ROS production, possibly H 2 O 2 , and subsequent cytochrome c release from mitochondria play an important role in mediating CHE-induced rapid cardiac myocyte apoptosis. CHE was shown to be a useful positive control for determination of cardiac myocyte apoptosis and a model for explaining the signalling mechanism including potent apoptosis in cardiac myocytes 44 . It has been reported that SA induced apoptosis which depends on the production of nitric oxide (NO) and superoxide radicals in prostate cancer cells 35 .…”

Section: Che-/sa-mediated Apoptosis Induced By Generation Of Reactivementioning

confidence: 99%

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Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Malíková

Zdařilová²,

Hlobílková³

2006

BIOMED PAP

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“…induction of apoptosis through generation of reactive oxygen 4) and stimulation of GSH transport. 5) Recently, chelerythrine was also proved to inhibit the BclXL function 6) and it was considered to open up new therapeutic treatments, while the diverse modification of chemical structures has not been systematically exploited yet.…”

mentioning

confidence: 99%

Synthesis of Oxychelerythrine Using Lithiated Toluamide-Benzonitrile Cycloaddition

Cho

2005

CHEMICAL & PHARMACEUTICAL BULLETIN

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Chelerythrine Rapidly Induces Apoptosis through Generation of Reactive Oxygen Species in Cardiac Myocytes

Cited by 111 publications

References 52 publications

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

Effects of Sanguinarine and Chelerythrine on the Cell Cycle and Apoptosis

Synthesis of Oxychelerythrine Using Lithiated Toluamide-Benzonitrile Cycloaddition

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