Chelerythrine Ameliorates Acetic Acid-Induced Ulcerative Colitis via Suppression of Inflammation and Oxidation

Abstract

The incidence of ulcerative colitis (UC), an inflammatory bowel disease (IBD), is drastically increasing globally, leading to greater economic hardship and patient morbidity. This study evaluated the effects of chelerythrine on acetic acid (AA)-induced UC in a mouse model. Mice received chelerythrine (25, 50, and 100 mg/kg, i.g.) and sulfasalazine (SASP) (500 mg/kg, i.g.) for 7 consecutive days following the induction of colitis via the intrarectal administration of AA (5% v/v). Disease activity index (DAI), tissue morphology, tissue damage, oxidative stress (OS), and inflammatory markers were then evaluated. The results revealed that the colonic levels of total antioxidant capacity (T-AOC), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) had decreased, while colonic myeloperoxidase (MPO), malondialdehyde (MDA), lipid peroxide (LPO) activity, colonic and serum interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α) levels, and serum lactate dehydrogenase (LDH) had all increased significantly in the colitis untreated group compared to that of the normal control. Treatment with chelerythrine considerably improved most of these parameters, and a dose of 100 mg/kg, was found to be comparable to that of 500 mg/kg SASP. This alkaloid also markedly reversed body weight (BW), clinical activity score, gross lesion score, mucosal edema, and colon weight/length in a dose-dependent manner. In conclusion, the protective effect of chelerythrine could be attributed to its anti-inflammatory and antioxidant activities.

Natural compounds as lactate dehydrogenase inhibitors: potential therapeutics for lactate dehydrogenase inhibitors-related diseases

“…Leonurine, derived from the Lamiaceae family, exhibits cardioprotective effects by decreasing LDH activity and exerting anti-oxidative activity ( Liu et al, 2010 ). Additionally, rutaecarpine, chelerythrine, and matrine have been shown to inhibit LDH levels ( Bao et al, 2011 ; Chen and Huang, 2012 ; Wu et al, 2022 ). Of these, rutaecarpine confers protection against myocardial cell injury by inhibiting the NADPH oxidase–ROS pathway ( Tian et al, 2019 ).…”

Anti-ulcerative colitis effects of chemically characterized extracts from Calliandra haematocephala in acetic acid-induced ulcerative colitis

“…In the context of this scenario, inflammation and oxidative stress are claimed to sketch UC ( Bastaki et al, 2022 ). These underlying mechanisms result in pathological features of UC including macroscopic damage such as swelling, edema, erosion, and bleeding and microscopic alterations such as cellular infiltration, and necrosis of the colon ultimately resulting in the clinical course of the disease ( Wu et al, 2022 ). Clinically, this disease is presented by abdominal pain, diarrhea, melena, weight loss and in complicated cases it can take transformation into carcinoma of colon ( Elbastawisy et al, 2022 ).…”