Chelating polymer-based membranes. Preparation and use for metal ion scavenging and sorption of murine immunoglobulin G by immobilized Ni(II) ions

Immobilization of horseradish peroxidase on modified chitosan beads

“…In order to attach the enzyme to the copolymer surface covalently, the support had to be activated using the Coulet method [9,11,22]. A variety of procedures has been reported for activating graft copolymers for enzyme immobilization [7][8][9][10][11][12][13]. The Coulet's method for binding the enzyme is particularly mild since the enzymes never come into contact with the chemical reagents, and, thus, denaturing processes are avoided.…”

“…Operative binding forces vary between weak multiple adsorptive interactions and single attachments [7,8] through strong covalent binding [9][10][11][12][13]. Which of the methods is the most appropriate is usually a matter of the desired applications.…”