Chelating of iron and copper alters properties of DNA in L5178Y cells, as revealed by the comet assay

Regulation of the nuclear proteasome activity in myelomonocytic human leukemia cells after adriamycin treatment

“…42,43 Alkaline single-cell gel electrophoresis was performed as described by Singh et al 44 Briefly, the cells were centrifuged, resuspended in PBS/0.5% low melting point agarose at 37°C, and 75 L of the mixture was added onto a frosted glass microscope slide precoated with 100 L of 1% melting point agarose. The slides were incubated 4°C for 10 minutes to allow polymerization of the agarose.…”

Forensic touch DNA recovery from metal surfaces – A review

“…M + binding can cause single strand break (ssb) or double strand breaks (dsb) [21]. Complex intercalation from transition metals can alter the double helix [26], causing damage via oxidative stress when bound to GC rich sites. The M + binding sites (arrowed) in Adenine: N1, N3 and N7; Guanine: N3, N7 and O6; Cytosine: N3 and O2 and; Thymine: O2 and O4, disrupts DNA integrity [27].…”

“…Transition metals present the most complicated interaction due to their ability to form more than one cation with varied ionic charges and subsequent multi-site binding activity with DNA [20]. Through chemical reactions with the N3 atom of pyrimidine (Cytosine or Thymine) or the N7 of purine (Adenine or Guanine), transition metals can alter the double helix [26] and their binding to GC rich sites has been reported to cause in vivo oxidative damage to DNA via H 2 O 2 generated radicals [39]. The coordinated complexes forming feature of these metals facilitates direct and indirect binding to nucleobases and phosphate groups, respectively [21].…”